CD3 抗体 (FITC)
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- 抗原 See all CD3 抗体
- CD3
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适用
- 猕猴
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD3 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS)
- 纯化方法
- Affinity Chromatography on Protein A
- 免疫原
- Rhesus monkey peripheral blood leucocytes. Spleen cells from immunised mice were fused with cells of the mouse SP2/0 Ag14 myeloma cell line.
- 克隆位点
- FN-18
- 亚型
- IgG1
- Top Product
- Discover our top product CD3 Primary Antibody
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anti-CD3 (CD3) antibody
CD3 适用: 人, 小鼠, 大鼠 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), ICC, CyTOF 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, CyTOF 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, Func, CyTOF 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), Func, CyTOF 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-57 Biotin
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal UCHT1 Biotin
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PerCP
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-57 PerCP
anti-CD3 (CD3) antibody (APC)CD3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-57 APC
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- 应用备注
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Flow cytometry: 1/10, Use 50 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.5 mL distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09 % Sodium Azide and 1 % BSA is recommended.
- 缓冲液
- PBS containing 1 % BSA and 125 mM Trehalose
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- CD3
- 别名
- CD3 (CD3 产品)
- 背景
- T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
- 基因ID
- 9541
- UniProt
- Q95LI5
- 途径
- TCR Signaling, Ubiquitin Proteasome Pathway
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