CD16 抗体
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- 抗原 See all CD16 抗体
- CD16
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD16 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunoprecipitation (IP)
- 特异性
- This antibody is a 50-65 kDa cell surface molecule which exists in two forms - a transmembranous form expressed by NK cells and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. This antibody has been reported to block the binding of IgG to the CD16 Molecule (Ref.4)
- 交叉反应 (详细)
- Species reactivity (tested):Human.
- 纯化方法
- Ion Exchange Chromatography
- 内毒素水平
- Low Endotoxin
- 免疫原
- Normal Human peripheral blood granulocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
- 克隆位点
- LNK16
- 亚型
- IgG1
- Top Product
- Discover our top product CD16 Primary Antibody
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- 应用备注
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Flow cytometry: 1/100 - 1/200, use 10 μL of suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01Eu/ug - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4, None, None
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- 抗原
- CD16
- 别名
- CD16 (CD16 产品)
- 背景
- CD16 (FcgammaRIII) serves as a low affinity IgG receptor. Human CD16 is expressed in two forms CD16a and CD16b. CD16a is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. CD16b is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.Synonyms: CD16A, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A
- 基因ID
- 2214
- NCBI登录号
- NP_000560
- UniProt
- P08637
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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