Biotin 抗体 (Texas Red (TR))
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- 抗原 See all Biotin products
- Biotin
- 适用
- 请咨询
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This Biotin antibody is conjugated to Texas Red (TR)
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 产品特性
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Absorption / Emission: 596 nm / 620 nm
Molar Ratio: 4.8 Moles Texas RedTM per mole of Goat IgG F(ab')2. - 纯化方法
- Immunoaffinity chromatography
- 免疫原
- Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
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anti-Biotin antibody
WB, ELISA, FACS, IHC, IF, FM 宿主: 小鼠 Monoclonal 4C7-D5 unconjugated
anti-Biotin antibodyWB, FACS, IHC, IF, ISt, CM 宿主: 小鼠 Monoclonal Hyb-8 unconjugated
anti-Biotin antibodyIHC, ISt 宿主: 兔 Monoclonal BTN-2032R unconjugated Recombinant Antibody
anti-Biotin antibodyWB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Biotin antibody (DyLight 488)WB, FACS, IF, FM, FLISA, DB, MA 宿主: 山羊 Polyclonal DyLight 488
anti-Biotin antibodyELISA, FACS 宿主: 小鼠 Monoclonal SB58c unconjugated
anti-Biotin antibodyELISA, FACS, IP, CyTOF 宿主: 小鼠 Monoclonal 1D4-C5 unconjugated
anti-Biotin antibody适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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- 应用备注
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Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring lot-to-lot consistency. Flow cytometry: 1.2,000 - 1: 10,000. IF microscopy: 1: 500 - 1: 2,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. Restore with 0.5 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- Biotin
- Abstract
- Biotin 产品
- 物质类
- Chemical
- 背景
- Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
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