Histone 抗体
Quick Overview for Histone 抗体 (ABIN1171837)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Polyclonal Antibody to Histone (H)
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特异性
- The antibody is a rabbit polyclonal antibody raised against H. It has been selected for its ability to recognize H in immunohistochemical staining and western blotting.
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纯化方法
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
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免疫原
- The antibody is a rabbit polyclonal antibody raised against HIS.
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亚型
- IgG
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anti-Histone antibody
适用: 人, 大鼠, 小鼠 WB, IHC (p), FACS, IF 宿主: 小鼠 Monoclonal AE-4 unconjugated
anti-Histone antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.
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说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
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储存液
- ProClin, Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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注意事项
- Avoid repeated freeze/thaw cycles
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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有效期
- 12 months
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- Histone
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背景
- HST
抗原
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