IL-4 抗体
Quick Overview for IL-4 抗体 (ABIN115763)
抗原
See all IL-4 (IL4) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Reacts with Interleukin 4.
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纯化方法
- Protein A chromatography
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免疫原
- Highly pure (>98%) recombinant human IL-4
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亚型
- IgG1
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anti-Interleukin 4 (IL4) (AA 25-153) antibody
IL4 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Interleukin 4 (IL4) (AA 25-153) antibodyIL4 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 122-150) antibodyIL4 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 61-111) antibodyIL4 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Interleukin 4 (IL4) (AA 25-153) antibodyIL4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (Internal Region) antibodyIL4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 122-150), (AA 122-151), (C-Term) antibodyIL4 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB22885 unconjugated
anti-Interleukin 4 (IL4) antibodyIL4 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) (AA 26-136) antibodyIL4 适用: 小鸡 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 4 (IL4) antibody (PE)IL4 适用: 人, 猕猴 FACS 宿主: 大鼠 Monoclonal MP4-25D2 PE
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应用备注
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ELISA: A 1: 10,000 dilution of a 1 mg/mL antibody solution will give satisfactory results in anELISA using a rabbit anti-mouse second antibody conjugated to alkaline phosphatase. Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity ofhIL-4 (0.5 ng/mL), a concentration of 3.0-4.5 μg/mL is required. Western blot: Use at a concentration of 0.20-0.40 μg/mL. Used in conjunction withcompatible secondary reagents the detection limit for recombinant hIL-4 is 0.25-0.50 ng/lane, under non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore in sterile water to a concentration of 1.0 mg/mL.
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缓冲液
- PBS without preservatives
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储存液
- Without preservative
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- IL-4 (IL4) (Interleukin 4 (IL4))
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别名
- Interleukin-4 / IL4
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背景
- IL4 is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. It is a ligand for Interleukin 4 receptor. The Interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. IL4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naive CD4+ T cell to the Th2 type. Th2 cells produce IL4, IL5, IL10 and IL13, which tend to favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. The second is regulating IgE and IgG1 production by B cells. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.Synonyms: B-cell stimulatory factor 1, BSF1, Binetrakin, IL-4, Lymphocyte stimulatory factor 1, Pitrakinra
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基因ID
- 3565
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NCBI登录号
- NP_000580
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UniProt
- P05112
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
抗原
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