CD3 抗体 (FITC)
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Quick Overview for CD3 抗体 (FITC) (ABIN114140)
抗原
See all CD3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This anti-mouse CD3ε monoclonal antibody is specific for the ε chain of mouse CD31. Immobolized 500A2 mAb can be used to stimulate mouse T cells in vitro1. A combination of immobolized 500A2 mAb and anti-mouse CD28 mAb is commonly used for the activation of mouse T cells3.
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免疫原
- C6VL-BS cell lysate
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亚型
- IgG
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anti-CD3 (CD3) antibody
CD3 适用: 人, 小鼠, 大鼠 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), ICC, MS 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, MS 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), Func, MS 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, Func, MS 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-57 Biotin
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal UCHT1 Biotin
anti-CD3 (CD3) antibody (APC)CD3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-57 APC
anti-CD3 (CD3) antibody (PE)CD3 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-57 PE
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PerCP
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应用备注
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Flow Cytometry (see Protocols). (Reportet to be useful in immunoprecipitation,immunofluorescent staining and immunohistochemistry on acetone fixed frozen sections. )
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- FLOW CYTOMETRY ANALYSIS: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test).
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % NaN3 and EIA grade BSA as stabilizing protien to bring total protein concentration to 4-5 mg/mL
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- CD3
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别名
- CD3
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背景
- In the mouse, CD3 is highly expressed by mature T cells and at lower levels by CD4+CD8+ thymocytes2. The ε chain of CD3 is a component of the multimeric T cell receptor complex and is involved with signal transductionSynonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
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基因ID
- 12501
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NCBI登录号
- NP_031674
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UniProt
- P22646
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途径
- TCR Signaling, Ubiquitin Proteasome Pathway
抗原
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