Red Blood Cell 抗体
RBC
适用: 小鸡
AGG
宿主: 兔
Polyclonal
unconjugated
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- 抗原 See all Red Blood Cell (RBC) products
- Red Blood Cell (RBC)
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适用
- 小鸡
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Red Blood Cell antibody is un-conjugated
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应用范围
- Agglutination (AGG)
- Supplier Product No.
- 203-4139
- Supplier
- Rockland
- 原理
- Chicken Red Blood Cell RBC Antibody
- 产品特性
- Synonyms: rabbit Anti-chicken RBC antibody, Red blood cell antibody, Antibody for hemagglutination, rabbit anti-chicken red blood cell, rabbit antibody to chicken red blood cells (RBC), haemolysin, hemolysin, erythrocytes sensitizing agent, anti-erythrotyes, anti-erythrocytes antibody
- 纯化方法
- This product is an IgG fraction antibody purified from polyspecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
- 免疫原
- Optional[Immunogen]: Chicken washed pooled Red Blood Cells (RBC)
- 亚型
- IgG
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- 应用备注
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Application Note: Tested for agglutination of cells on titer plates. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Other: AGGLUTINATION TITER 1:32 - 1:64
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 5.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 10.0 mg/mL
- 缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- Red Blood Cell (RBC)
- 别名
- RBC (RBC 产品)
- 背景
- Background: Anti-CHICKEN Red Blood Cell Antibody may be used in hemagglutination assays. Haemagglutination assay or HA is a method of quantification for viruses or bacteria by hemagglutination. Some viral families and many bacteria have envelope or surface proteins which are able to agglutinate (stick to) human or animal red blood cells (RBC) and bind to N-acetylneuraminic acid. As each of the agglutinating molecule attaches to multiple RBCs, a lattice-structure will form. Normally, a virus dilution (e.g. 2-fold from 1:4 to 1:4096) will be applied to an RBC dilution (e.g. 0.1 % to 0.7 % in steps of 0.2 %) for approx. 30 min, often at 4° C, otherwise viruses with neuraminidase activity will detach the virus from the RBCs. Then the lattice forming parts will be counted and the titer calculated. The titer of a hemagglutination assay is determined by the last viable "lattice" structure found. This is because it is at the point where, if diluted anymore, the amount of Virus particles will be less than that of the RBCs and thus not be able to agglutinate them together. Anti-CHICKEN Red Blood Cell Antibody is used to sensitize erythrocytes and quantitate agglutination.
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