DARPP32 抗体 (AA 70-181)
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北京 101111
Quick Overview for DARPP32 抗体 (AA 70-181) (ABIN968572)
抗原
See all DARPP32 (PPP1R1B) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 70-181
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交叉反应
- 大鼠, 小鼠
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产品特性
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States. -
纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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免疫原
- Human DARPP-32 aa. 70-181
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亚型
- IgG1
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anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr34) antibody
PPP1R1B 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (AA 95-204) antibodyPPP1R1B 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2G6D9 unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (AA 1-36), (N-Term) antibodyPPP1R1B 适用: 人, 小鼠, 大鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr75) antibodyPPP1R1B 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (AA 1-168) antibodyPPP1R1B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1A3 unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) antibodyPPP1R1B 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) antibodyMicroarray verified PPP1R1B 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB211117 unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) antibodyMicroarray verified PPP1R1B 适用: 人, 小鼠 IHC 宿主: 小鼠 Monoclonal MAB349172 unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (AA 51-150) antibodyPPP1R1B 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (AA 106-139) antibodyPPP1R1B 适用: 小鼠 WB 宿主: 兔 Polyclonal RB53838 unconjugated
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 250 μg/mL
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缓冲液
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store undiluted at -20°C.
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: "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons." in: Nature, Vol. 402, Issue 6762, pp. 669-71, (2000) (PubMed).
: "D(2) dopamine receptors induce mitogen-activated protein kinase and cAMP response element-binding protein phosphorylation in neurons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 20, pp. 11607-12, (1999) (PubMed).
: "Protein phosphatase 1 modulation of neostriatal AMPA channels: regulation by DARPP-32 and spinophilin." in: Nature neuroscience, Vol. 2, Issue 1, pp. 13-7, (1999) (PubMed).
: "DARPP-32: regulator of the efficacy of dopaminergic neurotransmission." in: Science (New York, N.Y.), Vol. 281, Issue 5378, pp. 838-42, (1998) (PubMed).
: "Cloning of cDNA for DARPP-32, a dopamine- and cyclic AMP-regulated neuronal phosphoprotein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 2, pp. 508-17, (1988) (PubMed).
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- DARPP32 (PPP1R1B) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B))
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别名
- DARPP-32
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背景
- Dopaminergic signaling in midbrain neurons is essential to multiple brain functions and involves the activation of dopamine receptors,nsuch as D1 and D2, which regulate the phosphorylation state of DARPP-32 (dopamine and cyclic AMP-regulated phospho-protein of Mr = 32,000). D1 receptor ligation causes activation of PKA and phosphorylation of DARPP-32 at Thr-34, which converts DARPP-32 to a potent inhibitor of protein phosphatase 1 (PP1). In addition, DARPP-32 is converted to an inhibitor of PKA via phosphorylation at Thr-75 by cyclin-dependent kinase 5 (Cdk5). D2 receptor ligation inhibits PKA and activates protein phosphatase 2B/calcineurin causing dephosphorylation of DARPP-32. The major function of DARPP-32 may be to inhibit the activity of PP1, which controls the phosphorylation state of neurotransmitter receptors, ion channels, ion pumps, and transcription factors. DARPP-32 -/- mice are defective in the physiologicial and behavioral responses to dopamine. Thus, DARPP-32, a bifunctional signal transduction molecule that differentially controls a Ser/Thr kinase and a Ser/Thr phosphatase, is a critical element of dopaminergic neurotransmission and normal brain function.
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分子量
- 32 kDa
抗原
-
