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SMURF2 抗体 (C-Term)

This 兔多克隆 antibody specifically detects SMURF2 in WB, IF 和 IHC (p). It exhibits reactivity toward 人和大鼠.It has been mentioned in 1 publication

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (SMURF2 抗体 (C-Term))

IHC (p)

Hippocampal neurons were fixed at stage 3, stained with anti-Smurf2 (red) and anti-Kinesin-2 (green) antibodies, and analyzed by confocal microscopy. (SMURF2 抗体 (C-Term))

Hippocampal neurons were transfected 2 h after plating with expression vectors for EGFP, EGFP-tagged Par3-4N/2, Par3-PDZ2, Par3-PDZ3, Smurf2-HECT (HECT), Smurf2-HECT-C716A (HECT CA), and shRNA directed against mPar3 (Par3 RNAi), or vectors for the anti-Par3 shRNA and human Myc-Par3 (RNAi + h Par3) (green). (SMURF2 抗体 (C-Term))

4 images

(1 reference)

产品编号 ABIN388831

发货至: 中国

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Quick Overview for SMURF2 抗体 (C-Term) (ABIN388831)

抗原

See all SMURF2 抗体

SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

适用

人, 大鼠

宿主

兔

克隆类型

多克隆

标记

This SMURF2 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

RB4107-4108

Discover our top product SMURF2 Primary Antibody

抗原表位
16

7

7

7

5

2

2

2

1

1

1

1

1
AA 702-731, C-Term

预测反应

M, X

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This SMURF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 702-731 amino acids from the C-terminal region of human SMURF2.

亚型

Ig Fraction
anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 601-700) antibody
SMURF2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) antibody
SMURF2 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) antibody
KD Validated SMURF2 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB7225 unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 1-748) antibody
SMURF2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (Internal Region) antibody
SMURF2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) antibody
SMURF2 适用: 人 WB 宿主: 小鼠 Monoclonal 1826CT865-51-28 unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (N-Term) antibody
SMURF2 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (C-Term) antibody
SMURF2 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 601-700) antibody (AbBy Fluor® 750)
SMURF2 适用: 人, 小鼠, 大鼠 WB, FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 750

anti-SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) (AA 601-700) antibody (AbBy Fluor® 680)
SMURF2 适用: 人, 小鼠, 大鼠 WB, FACS, IF (cc), IF (p) 宿主: 兔 Polyclonal AbBy Fluor® 680

应用备注

IF: 1:10~50. IF: 1:10~50. WB: 1:2000. IHC-P: 1:50~100

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Schwamborn, Khazaei, Püschel: "The interaction of mPar3 with the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity." in: The Journal of biological chemistry, Vol. 282, Issue 48, pp. 35259-68, (2007) (PubMed).
抗原

SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))

别名

SMURF2

背景

SMURF2 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with SMAD1, SMAD2 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. It enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

分子量

86196

基因ID

64750

NCBI登录号

NP_073576

UniProt

Q9HAU4