PPP2R5D 抗体 (C-Term)
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Quick Overview for PPP2R5D 抗体 (C-Term) (ABIN184648)
抗原
See all PPP2R5D 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- PPP2R5D
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序列
- KRAEEFLTAS QEAL
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特异性
- This antibody is expected to recognise reported isoforms (NP_006236.1 (iso 1), NP_851307.1, (iso 2) NP_851308.1) (iso 3) and NP_001257405.1 (iso 4).
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交叉反应
- Cow, 犬, 人, 小鼠, Pig, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-KRAEEFLTASQEAL, from the C Terminus of the protein sequence according to NP_006236.1, NP_851307.1, NP_851308.1.
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亚型
- IgG
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应用备注
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Western Blot: Approx 70 kDa band observed in lysates of cell lines A431, HepG2 and Jurkat, and in Human Cerebellum lysates, approx. 65-70 kDa in lysates of cell line NIH3T3, and approx.75 kDa in Rat Brain lysates, (calculated MW of 70 kDa according to Huma
Peptide ELISA: antibody detection limit dilution 1:20000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- PPP2R5D (Protein Phosphatase 2, Regulatory Subunit B', delta (PPP2R5D))
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别名
- PPP2R5D
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背景
- PPP2R5D, MGC2134, MGC8949, protein phosphatase 2, regulatory subunit B (B56), delta isoform, PP2A, B subunit, B' delta isoform, PP2A, B subunit, R5 delta isoform, PP2A, B subunit, B56 delta isoform, PP2A, B subunit, PR61 delta isoform, Serine/threonine pr
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基因ID
- 5528
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NCBI登录号
- NP_006236, NP_851307, NP_851308
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途径
- PI3K-Akt Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades
抗原
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