LAT2 抗体 (AA 91-244)
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北京 101111
Quick Overview for LAT2 抗体 (AA 91-244) (ABIN120727)
抗原
See all LAT2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 91-244
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特异性
- The antibody reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.
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纯化方法
- Protein A affinity chromatography
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- Recombinant cytoplasmic domain (aa 91-244) of human NTAL
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亚型
- IgG1
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anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 91-243), (Cytoplasmic Domain) antibody
LAT2 适用: 人, 小鼠 WB, IP, ICC, ICFC 宿主: 小鼠 Monoclonal NAP-07 unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 30-209) antibodyLAT2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibodyLAT2 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 91-243), (Cytoplasmic Domain) antibody (PE)LAT2 适用: 人, 小鼠 ICFC 宿主: 小鼠 Monoclonal NAP-07 PE
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 32-192) antibodyLAT2 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 196-212) antibodyLAT2 适用: 人 WB, IHC, IP, IHC (p), Inhibition 宿主: 小鼠 Monoclonal NAP-03 unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibodyLAT2 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB14275 unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 124-203), (Cytoplasmic Domain), (N-Term) antibodyLAT2 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibodyLAT2 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB15935 unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibodyLAT2 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 24GB14270 unconjugated
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应用备注
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Immunoprecipitation. Western Blot: Recommended dilution: 2 mg/mL, incubation 60 min Positive control: RAMOS human lymphoma cell line Negative control: HeLa human cervix carcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12 % separating SDS-PAGE gel. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. -
有效期
- 12 months
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: "Molecular characterization of rat gastric mucosal response to potent acid inhibition." in: Physiological genomics, Vol. 22, Issue 1, pp. 24-32, (2007) (PubMed).
: "NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation." in: Blood, Vol. 104, Issue 1, pp. 207-14, (2004) (PubMed).
: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, (2004) (PubMed).
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- LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))
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别名
- LAT2 / WBSCR15
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背景
- NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein
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基因ID
- 7462
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UniProt
- Q9GZY6
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途径
- Fc-epsilon Receptor Signaling Pathway, BCR Signaling
抗原
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