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HLA Class I A-2 alpha (HLAA2) 抗体 (PE)

ABIN119578 产品详细信息, 供应商: Log in to see
抗原
适用

3
宿主
小鼠
3
克隆类型 (克隆位点)
单克隆 ()
标记
PE
1
应用范围
Flow Cytometry (FACS)
3
2
2
1
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免疫原 Papain solubilised HLA-A2
克隆位点 BB7-2
亚型 IgG2b
特异性 This antibody recognises the Human HLA-A2 histocompatability antigen. The epitope recognised by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. This antibody may be used for the Flow Cytometric Detection of HLA-A2 expression.
交叉反应 (详细) Species reactivity (tested):Human.
产品特性 Absorption / Emission: 488 nm / 575 nm
纯化方法 Affinity Chromatography on Protein G
别名 HLA Class I A-2 alpha / HLA-A2
背景 The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. The HLA A gene is part of the human HLA complex on chromosome 6 and there are a large number of variant alleles of this gene.Synonyms: A-2 alpha chain, HLA class I histocompatibility antigen, HLAA2, MHC class I antigen A-2
基因ID 100507703
NCBI登录号 XP_003119274
UniProt P01892
应用备注 Flow Cytometry (use 10 μL of neat antibody to label 10^6 cells or 100 μL whole blood).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
溶解方式 Restore with 1.0 mL distilled water.
浓度 0.1 mg/mL
缓冲液 PBS, 0.09 % Sodium Azide, 1 % BSA and 5 % Sucrose
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody at 2-8 °C for one month or at -20 °C for longer.
厂商提供的图像
Flow Cytometry (FACS) image for anti-HLA Class I A-2 alpha (HLAA2) 抗体 (PE) (ABIN119578) Staining of human peripheral blood lymphocytes with mouse anti human HLA-A2 - RPE ant...
背景 Schmidt, Guillaume, Irving, Baumgaertner, Speiser, Luescher: "Reversible major histocompatibility complex I-peptide multimers containing Ni(2+)-nitrilotriacetic acid peptides and histidine tags improve analysis and sorting of CD8(+) T cells." in: The Journal of biological chemistry, Vol. 286, Issue 48, pp. 41723-35, 2011 (PubMed).

Pantenburg, Castellanos-Gonzalez, Dann, Connelly, Lewis, Ward, White: "Human CD8(+) T cells clear Cryptosporidium parvum from infected intestinal epithelial cells." in: The American journal of tropical medicine and hygiene, Vol. 82, Issue 4, pp. 600-7, 2010 (PubMed).

Duncan, Nathan, Lehner: "Stabilization of an E3 ligase-E2-ubiquitin complex increases cell surface MHC class I expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 12, pp. 6978-85, 2010 (PubMed).

Wooldridge, Clement, Lissina, Edwards, Ladell, Ekeruche, Hewitt, Laugel, Gostick, Cole, Debets, Berrevoets, Miles, Burrows, Price, Sewell: "MHC class I molecules with Superenhanced CD8 binding properties bypass the requirement for cognate TCR recognition and nonspecifically activate CTLs." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 184, Issue 7, pp. 3357-66, 2010 (PubMed).

Wooldridge, Lissina, Vernazza, Gostick, Laugel, Hutchinson, Mirza, Dunbar, Boulter, Glick, Cerundolo, van den Berg, Price, Sewell: "Enhanced immunogenicity of CTL antigens through mutation of the CD8 binding MHC class I invariant region." in: European journal of immunology, Vol. 37, Issue 5, pp. 1323-33, 2007 (PubMed).

Wang, Chen, Jiang, Zhang, Wan, Li, Zhou, Wu, Yang, Yu, Wang, Yang, Cao: "Identification of an HLA-A*0201-restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein." in: Blood, Vol. 104, Issue 1, pp. 200-6, 2004 (PubMed).

Harig, Witzens, Krackhardt, Trojan, Barrett, Broderick, Zauls, Gribben: "Induction of cytotoxic T-cell responses against immunoglobulin V region-derived peptides modified at human leukocyte antigen-A2 binding residues." in: Blood, Vol. 98, Issue 10, pp. 2999-3005, 2001 (PubMed).

Hogan, Brown: "Localization and characterization of serologic epitopes on HLA-A2." in: Human immunology, Vol. 33, Issue 3, pp. 185-92, 1992 (PubMed).

Parham, Brodsky: "Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28." in: Human immunology, Vol. 3, Issue 4, pp. 277-99, 1982 (PubMed).

Roy-Camille, Lelièvre, Saillant: "[Treatment of hypercorrection after surgical procedure on hallux valgus. Medial osteoplastic ridge (author's transl)]." in: La Nouvelle presse médicale, Vol. 7, Issue 37, pp. 3357-8, 1979 (PubMed).