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Podoplanin 抗体 (PDPN)

Details for Product anti-PDPN Antibody No. ABIN115146, 供应商: Log in to see
抗原
  • PDPN
  • AGGRUS
  • GP36
  • GP40
  • Gp38
  • HT1A-1
  • OTS8
  • PA2.26
  • T1A
  • T1A-2
  • OTS-8
  • RANDAM-2
  • T1-alpha
  • T1a
  • T1alpha
  • E11
  • RTI40
  • podoplanin
  • podoplanin-like
  • PDPN
  • Pdpn
  • LOC100738269
Alternatives
anti-人 Podoplanin 抗体 for Cytometry by Time of Flight
适用

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克隆类型 (克隆位点)
单克隆 ()
标记
This Podoplanin antibody is un-conjugated
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应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Supplier
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免疫原 GP36 (Podoplanin) expressed by MDCK cells.
克隆位点 18H5
亚型 IgG1
特异性 This antibody reacts with Podoplanin (gp36).
交叉反应 (详细) Species reactivity (tested):Human.
纯化方法 Affinity Chromatography on Protein G.
别名 Podoplanin (PDPN Antibody 摘要)
背景 Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47 % aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.Synonyms: Aggrus, GP36, Glycoprotein 36, PA2.26 antigen, PDPN, PSEC0003, PSEC0025, T1-alpha
基因ID 10630
NCBI登录号 NP_001006625
UniProt Q86YL7
途径 Dicarboxylic Acid Transport
应用备注 FACS analysis (3-20 μg/mL). Immuofluorescence (1-2 μg/mL). Western blot analysis (1-2 μg/mL). Immunohistochemistry on Frozen and Paraffin Sections (6-30 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程 Sample protocol for Formalin-Fixed Paraffin-Embedded Sections (as a guide only)1) Put Paraffin sections (2-4 µm) onto a Poly-Lysin object holder2) Deparaffination: - Xylol (10 min)- Acetone (10 min)- Acetone / buffer mixture (10 min)- Rinse 3x with wash buffer3) Microwave: boil up 3x 10 min in the appropriate buffer (put the whole glass curette intothe microwave)a) EDTA buffer: 0,1 M EDTA in aqua dest, pH 8,0b) Citrate buffer/10 mM, pH 6,0A: citric acid, 19,2 g in 1L aqua destB: Na-Citrate, 29,41 g in 1L aqua dest9 ml stock solution A + 50 ml stock solution B, in 500 ml aqua dest (pH adjustment only insolution B)Cool down the sections to RT for 25 min in wash buffer4) Addition of primary ABs (dilute e. g. in ready to use buffer from DAKO [S3022])- Shortly drip off the sections on paper (take 3-4 sections at once)- Dust off the liquid beside the sections- Add primary Abs- incubate 0. 5 h at RT- Rinse 3x with wash buffer- Shortly drip off the sections5) Addition of the by-pass AB (monoclonal mouse-anti-rabbit IgG, e. g. DAKO [M0737] 1: 250)- incubate 0. 5 h at RT- Rinse 3x with wash buffer- Shortly drip off the sections6) Addition of anti-mouse-IgG from rabbit 1: - Incubate 0. 5 h at RT- Rinse 3x with wash buffer- Shortly drip off the sections7)Addition of the APAAP complexes- Incubate 0. 5 h at RT
限制 仅限研究用
溶解方式 Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
缓冲液 PBS, pH 7.4 without preservatives or stabilizers
储存液 Without preservative
注意事项 This product is photosensitive and should be protected from light
储存条件 4 °C
储存方法 Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
厂商提供的图像
Flow Cytometry (FACS) image for anti-Podoplanin 抗体 (PDPN) (ABIN115146) FACS analysis of Podoplanin in human microvascular endothelial cells using antibody A...
有引用在: Birke, Lütjen-Drecoll, Kerjaschki, Birke: "Expression of podoplanin and other lymphatic markers in the human anterior eye segment." in: Investigative ophthalmology & visual science, Vol. 51, Issue 1, pp. 344-54, 2010 (PubMed).

Conrad, Niess, Huss, Huber, von Luettichau, Nelson, Ott, Jauch, Bruns: "Multipotent mesenchymal stem cells acquire a lymphendothelial phenotype and enhance lymphatic regeneration in vivo." in: Circulation, Vol. 119, Issue 2, pp. 281-9, 2009 (PubMed).

Turner, Langley, Johnston, Gentil, Ford, Wu, Graham, Sharpley, Slatko, Pearlman, Taylor: "Wolbachia lipoprotein stimulates innate and adaptive immunity through Toll-like receptors 2 and 6 to induce disease manifestations of filariasis." in: The Journal of biological chemistry, Vol. 284, Issue 33, pp. 22364-78, 2009 (PubMed).

Kilic, Oliveira-Ferrer, Neshat-Vahid, Irmak, Obst-Pernberg, Wurmbach, Loges, Kilic, Weil, Lauke, Tilki, Singer, Ergün: "Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1." in: Blood, Vol. 110, Issue 13, pp. 4223-33, 2007 (PubMed).

Riesenberg, Weiler, Spring, Eder, Buchner, Popp, Castro, Kammerer, Takikawa, Hatz, Stief, Hofstetter, Zimmermann: "Expression of indoleamine 2,3-dioxygenase in tumor endothelial cells correlates with long-term survival of patients with renal cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 23, pp. 6993-7002, 2007 (PubMed).

背景 Schacht, Ramirez, Hong, Hirakawa, Feng, Harvey, Williams, Dvorak, Dvorak, Oliver, Detmar: "T1alpha/podoplanin deficiency disrupts normal lymphatic vasculature formation and causes lymphedema." in: The EMBO journal, Vol. 22, Issue 14, pp. 3546-56, 2003 (PubMed).

Zimmer, Oeffner, Von Messling, Tschernig, Gröness, Klenk, Herrler: "Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium." in: The Biochemical journal, Vol. 341 ( Pt 2), pp. 277-84, 1999 (PubMed).

Breiteneder-Geleff, Matsui, Soleiman, Meraner, Poczewski, Kalt, Schaffner, Kerjaschki: "Podoplanin, novel 43-kd membrane protein of glomerular epithelial cells, is down-regulated in puromycin nephrosis." in: The American journal of pathology, Vol. 151, Issue 4, pp. 1141-52, 1997 (PubMed).