Keratin 19 (KRT19) 抗体

ABIN114797 产品详细信息, 供应商: Log in to see
抗原
  • k19
  • ck19
  • k1cs
  • krt9
  • krt15
  • MGC76282
  • MGC83069
  • KRT19
  • CK19
  • K19
  • K1CS
  • GK-19
  • Ka19
  • Krt1-19
  • AI663979
  • EndoC
  • Krt-1.19
  • keratin 19
  • KRT19
  • krt19
  • Krt19
适用

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宿主
小鼠
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克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
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应用范围
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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选项
Supplier
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Supplier Product No.
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免疫原 Human breast cancer cell line MCF-7
克隆位点 Ks19-1 (A53-B-A2)
亚型 IgG2a
特异性 Specific for the rod domain (aa 312-335) of Human Cytokeratin 19. In Western blotting 40 kDa and 19 kDa bands are observed.
交叉反应 (详细) Species reactivity (tested):Human.
纯化方法 Affinity Chromatography on Protein G.
别名 Cytokeratin 19 (KRT19 Antibody 摘要)
背景 This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumours. Keratin 19 is often co-expressed with keratin 7.Synonyms: CK19, Cytokeratin-19, K19, KRT19, Keratin type I cytoskeletal 19, Keratin-19
基因ID 3880
NCBI登录号 NP_002267
UniProt P08727
研究领域 Signaling, Cytoskeleton, Cytokeratins, Extracellular Matrix
应用备注 ELISA. Western blot. Immunofluorescence. Immunohistochemistry on Paraffin Sections: 1/100-1/200 (Requires protein digestionpre-treatment of paraffin sections e. g. trypsin or pronase). Use appendix as Positive Control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 1.0 mg/mL
缓冲液 PBS, 0.09 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Keratin 19 (KRT19) antibody (ABIN114797) Staining of paraffin embedded human appendix with mouse anti human cytokeratin 19 (AB...
背景 Goletz, Hanisch, Karsten: "Novel alphaGalNAc containing glycans on cytokeratins are recognized invitro by galectins with type II carbohydrate recognition domains." in: Journal of cell science, Vol. 110 ( Pt 14), pp. 1585-96, 1997 (PubMed).

Kasper, Stosiek, Typlt, Karsten: "Histological evaluation of three new monoclonal anti-cytokeratin antibodies. 1. Normal tissues." in: European journal of cancer & clinical oncology, Vol. 23, Issue 2, pp. 137-47, 1988 (PubMed).

Karsten, Papsdorf, Roloff, Stolley, Abel, Walther, Weiss: "Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion." in: European journal of cancer & clinical oncology, Vol. 21, Issue 6, pp. 733-40, 1985 (PubMed).