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Cytokeratin 19 抗体

The 小鼠 单克隆 anti-Cytokeratin 19 antibody is suitable to detect Cytokeratin 19 in samples from 人. It has been validated for WB, IF 和 IHC (p).
产品编号 ABIN114797
发货至: 中国
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Cytokeratin 19 抗体 (ABIN114797)

抗原

See all Cytokeratin 19 (KRT19) 抗体
Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

适用

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小鼠

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单克隆

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This Cytokeratin 19 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

Ks19-1 (A53-B-A2)
  • 特异性

    Specific for the rod domain (aa 312-335) of Human Cytokeratin 19. In Western blotting 40 kDa and 19 kDa bands are observed.

    交叉反应 (详细)

    Species reactivity (tested):Human.

    纯化方法

    Affinity Chromatography on Protein G.

    免疫原

    Human breast cancer cell line MCF-7

    亚型

    IgG2a
  • 应用备注

    ELISA. Western blot. Immunofluorescence. Immunohistochemistry on Paraffin Sections: 1/100-1/200 (Requires protein digestionpre-treatment of paraffin sections e. g. trypsin or pronase). Use appendix as Positive Control.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    PBS, 0.09 % Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

    别名

    Cytokeratin 19

    背景

    This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumours. Keratin 19 is often co-expressed with keratin 7.Synonyms: CK19, Cytokeratin-19, K19, KRT19, Keratin type I cytoskeletal 19, Keratin-19

    基因ID

    3880

    NCBI登录号

    NP_002267

    UniProt

    P08727
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