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Desmocollin 3 抗体

DSC3 适用: 人 WB, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal Dsc3-U114 unconjugated
产品编号 ABIN112239
发货至: 中国
  • 抗原 See all Desmocollin 3 (DSC3) 抗体
    Desmocollin 3 (DSC3)
    适用
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    宿主
    • 44
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    小鼠
    克隆类型
    • 45
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    单克隆
    标记
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    This Desmocollin 3 antibody is un-conjugated
    应用范围
    • 44
    • 19
    • 13
    • 5
    • 5
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    序列
    VHGAPFYFSL PNTSPEISRL WSLTKVC
    特异性
    This anibody localizes Dsc3 present in: - all living epidermal layers - in glandular duct cells - in basal matrix cells and outer root sheath of hair follicles - basal and suprabasal cell layers of stratified epithelia (e.g. vagina, tongue, esophagus), basal layer of bladder urothelium and complex epithelium of trachea - thymic reticulum cells All simple epithelia are negative. Intercalated disks of myocardium are negative. Polypeptide reacting: 109 kDa and 100 kDa polypeptides (desmocollin 3 splice isoforms at pI 5.2) from human epidermal desmosomes. Most tested cultured cell lines derivated from stratified squamous epithelia or squamous cell carcinoma are positive (e.g A-431, HaCat). Does not react with Bovine and Rodent.
    纯化方法
    Affinity Chromatography on Protein A
    免疫原
    Synthetic peptide corresponding to a sequence present on the extracellular anchor domain of Human Desmocollin 3.
    克隆位点
    Dsc3-U114
    亚型
    IgG1
    Top Product
    Discover our top product DSC3 Primary Antibody
  • 应用备注
    Immunoblotting (Western). Immunohistochemistry on Frozen Sctions. Immunohistochemistry on Paraffin-Embedded Sections after microwave treatment: 1/10 inPBS ( pH 7.4) for 1h at RT. Recommendations: For better resolution on Frozen Sections preincubation (directly afterfixation) with 0.05-0.2 % Triton X100, for 5-10 min, depending on tissue type, isrecommended (Ref.2).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore in 1 mL distilled water.
    缓冲液
    PBS buffer, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    This product is photosensitive and should be protected from light
    储存条件
    4 °C
    储存方法
    Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
  • Lorén, Cabré, Ojanguren, Domènech, Pedrosa, García-Jaraquemada, Mañosa, Manyé et al.: "Interleukin-10 Enhances the Intestinal Epithelial Barrier in the Presence of Corticosteroids through p38 MAPK Activity in Caco-2 Monolayers: A Possible Mechanism for Steroid Responsiveness in ..." in: PLoS ONE, Vol. 10, Issue 6, pp. e0130921, (2016) (PubMed).

    Brenner, Riha, Giessrigl, Thalhammer, Grusch, Krupitza, Stieger, Jäger: "The effect of organic anion-transporting polypeptides 1B1, 1B3 and 2B1 on the antitumor activity of flavopiridol in breast cancer cells." in: International journal of oncology, Vol. 46, Issue 1, pp. 324-32, (2015) (PubMed).

    Hamidov, Altendorf-Hofmann, Chen, Settmacher, Petersen, Knösel: "Reduced expression of desmocollin 2 is an independent prognostic biomarker for shorter patients survival in pancreatic ductal adenocarcinoma." in: Journal of clinical pathology, Vol. 64, Issue 11, pp. 990-4, (2012) (PubMed).

    Cui, Chen, Yang, Mireskandari, Knösel, Zhang, Kohler, Kunze, Presselt, Petersen: "Diagnostic and prognostic impact of desmocollins in human lung cancer." in: Journal of clinical pathology, Vol. 65, Issue 12, pp. 1100-6, (2012) (PubMed).

    Goris, Uhlenbruck, Schwegmann-Wessels, Köhl, Niedorf, Stern, Hewicker-Trautwein, Bals, Taylor, Braun, Bicker, Kietzmann, Herrler: "Differential sensitivity of differentiated epithelial cells to respiratory viruses reveals different viral strategies of host infection." in: Journal of virology, Vol. 83, Issue 4, pp. 1962-8, (2009) (PubMed).

  • 抗原
    Desmocollin 3 (DSC3)
    别名
    Desmocollin-3 (DSC3 产品)
    别名
    CDHF3 antibody, DSC antibody, DSC1 antibody, DSC2 antibody, DSC4 antibody, HT-CP antibody, 5430426I24Rik antibody, MGC85083 antibody, desmocollin 3 antibody, desmocollin 3 S homeolog antibody, DSC3 antibody, Dsc3 antibody, dsc3.S antibody, dsc3 antibody
    背景
    Desmocollin 3 is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined.Synonyms: CDHF3, Cadherin family member 3, DSC3, DSC4, Desmocollin-4, HT-CP
    基因ID
    1825
    NCBI登录号
    NP_001932
    UniProt
    Q14574
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