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S100A9 抗体 (S100 Calcium Binding Protein A9)

Details for Product anti-S100A9 Antibody No. ABIN111886, 供应商: Log in to see
抗原
  • S100A9
  • 60B8AG
  • CAGB
  • CFAG
  • CGLB
  • L1AG
  • LIAG
  • MAC387
  • MIF
  • MRP14
  • NIF
  • P14
  • Mrp14
  • MRP-14
  • p14
  • 60B8Ag
  • AW546964
  • BEE22
  • Cagb
  • GAGB
  • L1Ag
  • S100 calcium binding protein A9
  • S100 calcium binding protein A9 (calgranulin B)
  • S100A9
  • S100a9
Alternatives
anti-人 S100A9 抗体 for Cytometry by Time of Flight
适用

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宿主
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克隆类型 (克隆位点)
单克隆 ()
标记
This S100A9 antibody is un-conjugated
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应用范围
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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Supplier
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免疫原 Cultured Human monocytes. Remarks: The antigen is MRP14, the epitope is suspected in the central region of the peptide.
克隆位点 S36-48
亚型 IgG1
特异性 Human MRP14, Granulocytes, stimulated Monocytes and Macrophages: This clone identifies the Ca2+-binding 14 kDa subunit of the inflammatory L-1 protein complex, also called S100A9 or Calgranulin B. It is useful for the characterization of circulating granulocytes or inflammatory infiltrates of the myelo-monocytic lineage which express MRP14 differently depending on the inflammatory status of the disease. Antigen Distribution Isolated Cells: The antigen is found in granulocytes and monocytes. It is absent from all other blood cells. In cultured monocytes, maximum MRP14 expression is found after 3-4 days. Myeloid leukaemic cells have been found to be positive as well. Tissue Sections: MRP-14 is found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP-14 increasingly during the early stages of inflammation. A high MRP-14 (and low MRP-8) expression by macrophages was reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases like chronic rheumatoid arthritis, MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8/14 determined by antibody 27E10 (product Cat.-No BM4025).Antigen Distribution
交叉反应 (详细) Species reactivity (tested):Human. The antibody reacts with Bovine spleen and not with Swine spleen.
纯化方法 Affinity Chromatography
别名 S100A9 / Calgranulin-B / MRP14 (S100A9 Antibody 摘要)
背景 S100A9 is a member of the S100 family of proteins. S100A9, together with S100A8 forms a heterodimeric protein complex, Calprotectin, which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. Complexes of S100A8 and S100A9 are the physiologically relevant forms of these proteins. S100A9 may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. Its expression and potential cytokine-like function in inflammation and in cancer suggest that S100A8/A9 may play a key role in inflammation-associated cancer.Synonyms: CAGB, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, MRP-14, Migration inhibitory factor-related protein 14, S100 calcium-binding protein A9, S100-A9
基因ID 9606
UniProt P06702
途径 Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity
应用备注 ELISA. Immunohistochemistry on Frozen Sections: 0.25 μg/mL (1/800)Immunohistochemistry on Paraffin Sections: 1 μg/mL (1/200). Proteinase K pre-treatmentfor antigen retrieval is recommended. SuggestedPpositive Control: Human tonsil. Has been described to work in FACS and Dot Blots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
实验流程 Protocol with Frozen, ice-cold Acetone-Fixed Sections: (The whole procedure is performed at room temperature)1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM
限制 仅限研究用
溶解方式 Restore in 0.5 mL distilled water.
浓度 0.2 mg/mL
缓冲液 PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.09 % Sodium Azide as a preservative
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody uundiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Should this product contain a precipitate we recommend microcentrifugation before use.
厂商提供的图像
Immunohistochemistry (IHC) image for anti-S100A9 抗体 (S100 Calcium Binding Protein A9) (ABIN111886) Immunohistochemical staining on human spleen paraffin sections using S100A9 antibody ...
背景 Wen, Xu, Mais, Goldman, McDonnell: "The A and B isoforms of the human progesterone receptor operate through distinct signaling pathways within target cells." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8356-64, 1994 (PubMed).

Teigelkamp, Bhardwaj, Roth, Meinardus-Hager, Karas, Sorg: "Calcium-dependent complex assembly of the myeloic differentiation proteins MRP-8 and MRP-14." in: The Journal of biological chemistry, Vol. 266, Issue 20, pp. 13462-7, 1991 (PubMed).

Delabie, de Wolf-Peeters, van den Oord, Desmet: "Differential expression of the calcium-binding proteins MRP8 and MRP14 in granulomatous conditions: an immunohistochemical study." in: Clinical and experimental immunology, Vol. 81, Issue 1, pp. 123-6, 1990 (PubMed).

Mues, Brisse, Zwadlo, Themann, Bender, Sorg: "Phenotyping of macrophages with monoclonal antibodies in endomyocardial biopsies as a new approach to diagnosis of myocarditis." in: European heart journal, Vol. 11, Issue 7, pp. 619-27, 1990 (PubMed).

Brandtzaeg, Jones, Flavell, Fagerhol: "Mac 387 antibody and detection of formalin resistant myelomonocytic L1 antigen." in: Journal of clinical pathology, Vol. 41, Issue 9, pp. 963-70, 1989 (PubMed).

Murao, Collart, Huberman: "A protein containing the cystic fibrosis antigen is an inhibitor of protein kinases." in: The Journal of biological chemistry, Vol. 264, Issue 14, pp. 8356-60, 1989 (PubMed).

Zwadlo, Brüggen, Gerhards, Schlegel, Sorg: "Two calcium-binding proteins associated with specific stages of myeloid cell differentiation are expressed by subsets of macrophages in inflammatory tissues." in: Clinical and experimental immunology, Vol. 72, Issue 3, pp. 510-5, 1988 (PubMed).

Odink, Cerletti, Brüggen, Clerc, Tarcsay, Zwadlo, Gerhards, Schlegel, Sorg: "Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis." in: Nature, Vol. 330, Issue 6143, pp. 80-2, 1987 (PubMed).