Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin. Tumors Specifically Detected: all adenocarcinoma, mammary carcinoma, urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellular carcinoma. Polypeptide Reacting: Mr 45 000 polypeptide (human keratin K18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reacts with HeLa and MCF-7 (cell lines tested so far).
纯化方法
Protein A Chromatography
免疫原
Human keratin K18 from a HeLa cytoskeletal preparation
Immunoflourescence. Cytological material. Flow Cytometry: 1/5-1/10. Immunohistochemistry of Frozen Sections. Immunohistochemistry of Paraffin Sections: 1/10. Working dilution: 1/10 for immunohistochemistry. Incubation Time: 1 h at 37 °C. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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背景
Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18.Synonyms: CK18, CYK18, Cell proliferation-inducing gene 46 protein, Cytokeratin-18, K18, KRT18, Keratin 18, Keratin type I cytoskeletal 18, Keratin-18