NEW: 请通过本地经销商订购 4A Biotech Co.,Ltd.

RuvC (Active) 蛋白

ABIN2452186 产品详细信息, 供应商: Log in to see
蛋白名称
宿主
大肠杆菌(E. Coli)
5
资源
大肠杆菌(E. Coli)
5
蛋白类型
Native
生物活性
Active
应用范围
Functional Studies (Func), SDS-PAGE (SDS), ELISA, Western Blotting (WB)
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

产品特性 Full-length recombinant protein expressed in E.coli and highly purified by combined chromatography. RuvC protein purity is over 90 % by SDS-PAGE (CBB staining)
背景 E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction leaving and resolving the recombinant. Functional form is dimmer.
UniProt P0A814
研究领域 Chromatin and Nuclear Signaling, DNA/RNA
应用备注 1) Functional studies in vitro. RuvC cleaves recombination intermediate at Holliday Junction.
2) SDS-PAGE (0. 2 g/lane)
3) Standard antigen for western blotting and ELISA
限制 仅限研究用
状态 Liquid
浓度 1.0 mg/mL
缓冲液 50 % glycerol, 10 mM Tris-HCl ( pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
储存条件 -20 °C/-80 °C
储存方法 Upon arrival centrifuge briefly and store at -20 C or at -80 C for longer storage.
厂商提供的图像
SDS-PAGE (SDS) image for RuvC (Active) 蛋白 (ABIN2452186) RuvC (Active) protein
有引用在: Murayama, Kurokawa, Mayanagi, Iwasaki: "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases." in: Nature, Vol. 451, Issue 7181, pp. 1018-21, 2008 (PubMed).

Shinagawa, Iwasaki: "Processing the holliday junction in homologous recombination." in: Trends in biochemical sciences, Vol. 21, Issue 3, pp. 107-11, 1996 (PubMed).

Iwasaki, Takahagi, Shiba, Nakata, Shinagawa: "Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure." in: The EMBO journal, Vol. 10, Issue 13, pp. 4381-9, 1992 (PubMed).