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RuvC 蛋白

RuvC 宿主: E. coli 宿主: 大肠杆菌(E. Coli) Native SDS, Func, ELISA, WB Active
产品编号 ABIN2452186
发货至: 中国
  • 抗原 See all RuvC products
    RuvC (Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
    蛋白类型
    Native
    生物活性
    Active
    宿主
    E. coli
    资源
    • 1
    大肠杆菌(E. Coli)
    应用范围
    SDS-PAGE (SDS), Functional Studies (Func), ELISA, Western Blotting (WB)
    产品特性
    Full-length recombinant protein expressed in E.coli and highly purified by combined chromatography. RuvC protein purity is over 90 % by SDS-PAGE (CBB staining)
  • 应用备注
    1) Functional studies in vitro. RuvC cleaves recombination intermediate at Holliday Junction.
    2) SDS-PAGE (0. 2 g/lane)
    3) Standard antigen for western blotting and ELISA
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液
    50 % glycerol, 10 mM Tris-HCl ( pH 7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol
    储存条件
    -20 °C/-80 °C
    储存方法
    Upon arrival centrifuge briefly and store at -20 C or at -80 C for longer storage.
  • Murayama, Kurokawa, Mayanagi, Iwasaki: "Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases." in: Nature, Vol. 451, Issue 7181, pp. 1018-21, (2008) (PubMed).

    Shinagawa, Iwasaki: "Processing the holliday junction in homologous recombination." in: Trends in biochemical sciences, Vol. 21, Issue 3, pp. 107-11, (1996) (PubMed).

    Iwasaki, Takahagi, Shiba, Nakata, Shinagawa: "Escherichia coli RuvC protein is an endonuclease that resolves the Holliday structure." in: The EMBO journal, Vol. 10, Issue 13, pp. 4381-9, (1992) (PubMed).

  • 抗原
    RuvC (Crossover Junction Endodeoxyribonuclease RuvC (RuvC))
    别名
    RuvC (RuvC 产品)
    别名
    ECK1864 Protein, JW1852 Protein, Crossover junction endodeoxyribonuclease RuvC Protein, ruvC Protein
    背景
    E. coli RuvC protein (19 kDa) is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction leaving and resolving the recombinant. Functional form is dimmer.
    UniProt
    P0A814
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