Protein Molecular Weight Marker

ABIN925595 产品详细信息, 供应商: Log in to see
检测范围
14-120 kDa
最低检测浓度
14 kDa
应用范围
SDS-PAGE (SDS), Western Blotting (WB)
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原理 This protein molecular weight marker is a cocktail containing seven purified proteins that range in size from 14 to 120 kDa and thus span a molecular weight range common to most proteins and protein subunits.
检测方法 Colorimetric
产品特性 The protein mixture has been formulated to yield well-defined bands after SDS-polyacrylamide gel electrophoresis and staining with Coomassie blue or other common staining techniques. The intensity of the 67 kDa bovine albumin band has been increased to serve as a reference. The product is supplied in 1X loading buffer and is ready-to-use.
过滤 Sterile filtered
应用备注 This product is designed for use in accurate estimation of protein molecular weight in SDS-polyacrylamide gel electrophoresis and western blotting. Thaw the cocktail at room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 5-10µl is appropriate for most gel formats. These are unstained markers optimized for use with Coomassie blue staining, but the loading volume can be adjusted for compatibility with other staining methods (i.e. silver staining). 62.5 mM Tris-HCl, pH 6.8, 2% (w/v) SDS, 250 mM NaCl, 1mM EDTA, 4% (v/v) β-Mercaptoethanol, 35% (v/v) Glycerol and 0.05% (w/v) bromophenol blue
说明

in 1x Loading Buffer

限制 仅限研究用
状态 Liquid
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
厂商提供的图像
 image for Protein Molecular Weight Marker (ABIN925595) Protein Molecular Weight Marker
 image for Protein Molecular Weight Marker (ABIN925595) Protein Molecular Weight Marker (Image 2)