Chemokine (C-C Motif) Ligand 8 (CCL8) ELISA 试剂盒

ABIN1706084 产品详细信息, 供应商: Log in to see
抗原
  • HC14
  • MCP-2
  • MCP2
  • SCYA10
  • SCYA8
  • 1810063B20Rik
  • AB023418
  • Mcp2
  • Scya8
  • chemokine (C-C motif) ligand 8
  • monocyte chemoattractant protein-2 precursor
  • CCL8
  • Ccl8
  • MCP-2
抗原表位
AA 20-97
适用
小鼠
其他选择
Kits with alternative reactivity to:
17
11
8
7
7
5
3
3
2
2
2
1
1
实验类型
Sandwich ELISA
检测范围
15.6-1000 pg/mL
最低检测浓度
15.6 pg/mL
应用范围
ELISA
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

原理 Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL8/MCP2
品牌 PicoKine™
样品类型 Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Analytical Method Quantitative
检测方法 Colorimetric
免疫原 Expression system for standard: E.coli
Immunogen sequence: E20-P97
特异性 Expression system for standard: E.coli
Immunogen sequence: E20-P97
交叉反应 (详细) There is no detectable cross-reactivity with other relevant proteins.
灵敏度 <10pg/mL
试剂未包括 Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
别名 CCL8 (CCL8 ELISA Kit 摘要)
背景

Protein Function: Chemotactic factor that attracts monocytes. This protein can bind heparin (By similarity). .

Background: Chemokine(C-C motif) ligand 8, also known as monocyte chemoattractant protein 2(MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2(MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.

Synonyms: C-C motif chemokine 8,Monocyte chemoattractant protein 2,Monocyte chemotactic protein 2,MCP-2,Small-inducible cytokine A8,Ccl8,Mcp2, Scya8,

Full Gene Name: C-C motif chemokine 8

Cellular Localisation: Secreted.
基因ID 20307
UniProt Q9Z121
应用备注 Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
板类型 Pre-coated
实验流程 mouse CCL8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL8 has been precoated onto 96-well plates. Standards(E.coli, E20-P97) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL8 amount of sample captured in plate.
实验流程

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse CCL8 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CCL8 standard solution and each sample is measured in duplicate.

实验精密度
  • Sample 1: n=16, Mean(pg/ml): 32, Standard deviation: 1.31, CV(%): 4.1
  • Sample 2: n=16, Mean(pg/ml): 125, Standard deviation: 5.88, CV(%): 4.7
  • Sample 3: n=16, Mean(pg/ml): 524, Standard deviation: 27.25, CV(%): 5.2,
  • Sample 1: n=24, Mean(pg/ml): 33, Standard deviation: 1.68, CV(%): 5.1
  • Sample 2: n=24, Mean(pg/ml): 129, Standard deviation: 8.39, CV(%): 6.5
  • Sample 3: n=24, Mean(pg/ml): 531, Standard deviation: 35.58, CV(%): 6.7
限制 仅限研究用
注意事项 Avoid multiple freeze-thaw cycles.
储存条件 -20 °C,4 °C
储存方法 Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
有效期 12 months
厂商提供的图像
ELISA image for Chemokine (C-C Motif) Ligand 8 (CCL8) ELISA 试剂盒 (ABIN1706084) Mouse CCL8/MCP2 PicoKine ELISA Kit standard curve
背景 Van Coillie, Fiten, Nomiyama, Sakaki, Miura, Yoshie, Van Damme, Opdenakker: "The human MCP-2 gene (SCYA8): cloning, sequence analysis, tissue expression, and assignment to the CC chemokine gene contig on chromosome 17q11.2." in: Genomics, Vol. 40, Issue 2, pp. 323-31, 1997 (PubMed).