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IL27 抗体 (Interleukin-27 Subunit alpha) (HRP)

Details for Product anti-IL27 Antibody No. ABIN5551711, 供应商: Log in to see
  • IL-27
  • IL-27p28
  • Il30
  • p28
  • IL-27A
  • IL27A
  • IL27p28
  • IL30
  • interleukin 27
  • Il27
  • IL27

This IL27 antibody is conjugated to HRP
Enzyme Immunoassay (EIA), Western Blotting (WB)
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免疫原 Full length recombinant mouse IL27/p28 protein
亚型 IgG
特异性 This antibody reacts to Interleukin-27A.
交叉反应 (详细) Species reactivity (expected):Rat.
Species reactivity (tested):Mouse.
产品特性 Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)
纯化方法 Ion exchange chromatography
别名 Interleukin-27 / IL27A
背景 The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-12 p35. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R. EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer. The heterodimeric IL-27 receptor contains WSX-1 (TCCR) and gp130. WSX-1 is specific for IL-27, and is expressed on resting/naive CD4+ T cells, CD8+ T cells, NK cells, dendritic cells, monocytes, mast cells, and B cells. Gp130, on the other hand, functions as a subunit of the receptor complexes for at least seven other cytokines. IL-27 also promotes effector functions of NK cells and CD8+ T cells.Synonyms: IL-27 subunit alpha, IL-30, IL27-A, IL30, Interleukin-30, p28
基因ID 246779
NCBI登录号 NP_663611
UniProt Q8K3I6
应用备注 ELISA: 1/10,000 - 1/50,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 1/500 - 1/2,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
溶解方式 Restore with 0.1 mL of deionized water (or equivalent).
浓度 1.0 mg/mL
缓冲液 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative (Do NOT add Sodium Azide!)
储存液 Gentamicin sulfate
注意事项 This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.