-
- 抗原 See all HNE 抗体
- HNE (4-Hydroxynonenal (HNE))
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This HNE antibody is un-conjugated
-
应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 特异性
- Specific for 4HNE-Lysine adduct in all species. This antibody has almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal, formaldehyde, or glutaraldehyde. By inhibition test, this antibody shows a much higher affinity for the 4-HNE-histidine adduct than 4-HNE-lysine or 4-HNE-cysteine adduct.
- 纯化方法
- Ammonium sulphate purified IgG
- 免疫原
- 4-Hydroxy-2-nonenal (4HNE) modified keyhole limpet hemocyanin.
- 克隆位点
- HNEJ-2
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product HNE Primary Antibody
-
-
- 应用备注
- IHC, Western and/or ELISA. Immunohistochemistry [ref.1] (Recommended concentration: 25 ug/mL IgG). Western blotting [ref.2] (Recommended concentration: 15 ug/mL IgG) User should determine optimum titer for each application.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Reconstitute with 200μL of distilled water.
- 缓冲液
- Lyophilized as 100 ug/mL IgG in 50 mM Tris buffered saline (TBS)
- 储存条件
- -20 °C
-
-
Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells." in: Molecular human reproduction, Vol. 16, Issue 3, pp. 188-99, (2010) (PubMed).
: "A human-type nonalcoholic steatohepatitis model with advanced fibrosis in rabbits." in: The American journal of pathology, Vol. 177, Issue 1, pp. 153-65, (2010) (PubMed).
: "Evaluation of lipid oxidative stress status and inflammation in atopic ocular surface disease." in: Molecular vision, Vol. 16, pp. 2465-75, (2010) (PubMed).
: "Role of Rac1 GTPase in NADPH oxidase activation and cognitive impairment following cerebral ischemia in the rat." in: PLoS ONE, Vol. 5, Issue 9, pp. e12606, (2010) (PubMed).
: "Renoprotective effect of azelnidipine in rats." in: Biological & pharmaceutical bulletin, Vol. 31, Issue 12, pp. 2237-44, (2008) (PubMed).
: "M-30 and 4HNE are sequestered in different aggresomes in the same hepatocytes." in: Experimental and molecular pathology, Vol. 83, Issue 3, pp. 296-300, (2007) (PubMed).
: "S-allyl cysteine prevents CCl(4)-induced acute liver injury in rats." in: Free radical research, Vol. 41, Issue 4, pp. 489-97, (2007) (PubMed).
: "Delayed loss of cone and remaining rod photoreceptor cells due to impairment of choroidal circulation after acute light exposure in rats." in: Investigative ophthalmology & visual science, Vol. 48, Issue 4, pp. 1864-72, (2007) (PubMed).
: "Thioredoxin may exert a protective effect against tissue damage caused by oxidative stress in salivary glands of patients with Sjögren's syndrome." in: The Journal of rheumatology, Vol. 34, Issue 10, pp. 2035-43, (2007) (PubMed).
: "Angiotensin II receptor type 1-mediated vascular oxidative stress and proinflammatory gene expression in aldosterone-induced hypertension: the possible role of local renin-angiotensin system." in: Endocrinology, Vol. 148, Issue 4, pp. 1688-96, (2007) (PubMed).
: "Modulation of the purinergic P2X7 receptor attenuates lipopolysaccharide-mediated microglial activation and neuronal damage in inflamed brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 18, pp. 4957-68, (2007) (PubMed).
: "Protein modifications by 4-hydroxynonenal and 4-hydroxyhexenal in light-exposed rat retina." in: Investigative ophthalmology & visual science, Vol. 46, Issue 10, pp. 3859-68, (2005) (PubMed).
: "Increased expression of Leydig cell haem oxygenase-1 preserves spermatogenesis in varicocele." in: Human reproduction (Oxford, England), Vol. 20, Issue 9, pp. 2608-13, (2005) (PubMed).
: "Reduction of cerebral infarction in stroke-prone spontaneously hypertensive rats by statins associated with amelioration of oxidative stress." in: Stroke; a journal of cerebral circulation, Vol. 36, Issue 3, pp. 670-2, (2005) (PubMed).
: "Protein-bound 4-hydroxy-2-hexenal as a marker of oxidized n-3 polyunsaturated fatty acids." in: Journal of lipid research, Vol. 45, Issue 4, pp. 626-34, (2004) (PubMed).
: "Gene transfer of NAD(P)H oxidase inhibitor to the vascular adventitia attenuates medial smooth muscle hypertrophy." in: Circulation research, Vol. 95, Issue 6, pp. 587-94, (2004) (PubMed).
: "Androgenic regulation of oxidative stress in the rat prostate: involvement of NAD(P)H oxidases and antioxidant defense machinery during prostatic involution and regrowth." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2513-22, (2003) (PubMed).
: "Change of redox status and modulation by thiol replenishment in retinal photooxidative damage." in: Investigative ophthalmology & visual science, Vol. 43, Issue 7, pp. 2392-400, (2002) (PubMed).
: "In vitro ischemia-reperfusion injury in term human placenta as a model for oxidative stress in pathological pregnancies." in: The American journal of pathology, Vol. 159, Issue 3, pp. 1031-43, (2001) (PubMed).
: "Formation of 8-hydroxy-2'-deoxyguanosine and 4-hydroxy-2-nonenal-modified proteins in rat liver after ischemia-reperfusion: distinct localization of the two oxidatively modified products." in: Antioxidants & redox signaling, Vol. 2, Issue 1, pp. 127-36, (2001) (PubMed).
: "
-
Oxidative stress-induced S100B protein from placenta and amnion affects soluble Endoglin release from endothelial cells." in: Molecular human reproduction, Vol. 16, Issue 3, pp. 188-99, (2010) (PubMed).
-
- 抗原
- HNE (4-Hydroxynonenal (HNE))
- Abstract
- HNE 产品
- 物质类
- Chemical
- 背景
- During the peroxidation process, a variery of aldehydes are formed. 4-hydroxy-2-nonenal (4-HNE) is an alpha, beta unsatulated aldehyde that can be formed by peroxidation of omega-6 unsatulated fatty acids such as linoleic acid and arachidonic acid. Especially, 4-HNE formed inside living body is reported to originate from phospholipid-bound arachidonic acid. 4-HNE may be one of the most reliable biomarker of lipid peroxidation. This antibody shows almost negligible reactivity with proteins that were treated with other aldehydes, such as 2-nonenal, 2-hexenal, 1-hexanal, 4-hydroxy-2-hexenal,formaldehyde, or glutaraldehyde.
-