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小鼠 anti-人 Immunoglobulin J Polypeptide, Linker Protein For Immunoglobulin alpha and mu Polypeptides (IGJ) (Joining Chain) Antibody

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抗原
抗原表位
Joining Chain
5
3
2
2
1
1
1
适用

15
3
3
宿主
小鼠
11
3
1
克隆类型 (克隆位点)
单克隆 ()
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
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免疫原 Human J Chain. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
克隆位点 MC19-9
亚型 IgG1
特异性 The antibody reacts with Human J chain as tested by ELISA using purified Human Ig fragments as the solid phase target antigen. The antibody does not detect J chain in intact IgM in ELISA assays due to steric hindrance. In Western blotting techniques SM1013P detects J chain in IgM only in reducing conditions.
纯化方法 Affinity Chromatography on Protein A.
别名 Immunoglobulin J Chain
背景 Immunoglobulin J chain (IGJ) serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces larger polymers. It also helps to bind these immunoglobulins to secretory components.Synonyms: IGCJ, IGJ
基因ID 3512
NCBI登录号 NP_653247
UniProt P01591
应用备注 Immunohistochemistry on Frozen Sections (1/200). ELISA (20 μg/mL). Immunoprecipitation (20 μg/mL). Western blotting (only under Reducing Conditions).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用
浓度 1.0 mg/mL
缓冲液 TRIS buffered Saline containing 0.09 % Sodium Azide as preservative.
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
背景 Arnold, Wormald, Suter, Radcliffe, Harvey, Dwek, Rudd, Sim: "Human serum IgM glycosylation: identification of glycoforms that can bind to mannan-binding lectin." in: The Journal of biological chemistry, Vol. 280, Issue 32, pp. 29080-7, 2005 (PubMed).

有引用在: Leithäuser, Bäuerle, Huynh, Möller: "Isotype-switched immunoglobulin genes with a high load of somatic hypermutation and lack of ongoing mutational activity are prevalent in mediastinal B-cell lymphoma." in: Blood, Vol. 98, Issue 9, pp. 2762-70, 2001 (PubMed).