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Mouse spleen cell or bone marrow suspension is labeled with BD IMag™ anti-mouse TER119 Particles - DM according to the following Protocol. This labeled cell suspension is then placed within the magnetic field of the BD IMagnet (Cat. no. 552311). Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be collected (depleted fraction). The tube is then removed from the magnetic field and additional buffer is added for resuspension of the positive fraction. The magnetic separation steps are diagrammed in the Separation Flow Chart. Both depleted and positive fractions can be evaluated in downstream applications such as flow cytometry.