anti-人 WNT9A 抗体 for Immunohistochemistry (Paraffin-embedded Sections)

Recommended WNT9A Antibody (supplied by: Log in to see )

抗原
Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) 抗体
  • zgc:153617
  • wnt-9a
  • Wnt14
  • WNT14
  • WNT-14
  • Wnt family member 9A
  • wingless-type MMTV integration site family, member 9A
  • wingless-type MMTV integration site family member 9A S homeolog
  • WNT9A
  • wnt9a
  • wnt9a.S
  • Wnt9a
抗原表位
Internal Region
12
9
9
8
4
3
2
2
1
1
1
1
适用

73
29
24
1
宿主

63
9
克隆类型
多克隆
标记
This WNT9A antibody is un-conjugated
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
43
25
13
11
11
7
2
1
1
选项
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Available images

产品编号 ABIN1049487
$ 484.00
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.5 ABIN675533 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
7 ABIN1049488 IHC IHC (p) Rabbit N-Term Log in to see Polyclonal 0
7 ABIN1740843 IHC (p) Rabbit IgG Internal Region Log in to see Polyclonal 0
7 ABIN5590980 IHC (p) Rabbit Log in to see Polyclonal 0
5.5 ABIN675535 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal 1
4 ABIN1740844 IHC (p) Rabbit IgG N-Term Log in to see Polyclonal 0
4 ABIN5590981 IHC (p) Rabbit Log in to see Polyclonal 0
1 ABIN1109515 IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN675542 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN2764567 IHC (p) Rabbit N-Term Log in to see Polyclonal 0
1 ABIN2764568 IHC (p) Rabbit Internal Region Log in to see Polyclonal 0

General

抗原 Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) 抗体
抗原表位 Internal Region
(12), (9), (9), (8), (4), (3), (2), (2), (1), (1), (1), (1)
适用
(73), (29), (24), (1)
宿主
(63), (9)
克隆类型
多克隆
标记 This WNT9A antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(43), (25), (13), (11), (11), (7), (2), (1), (1)
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WNT9A抗体详情

WNT9A目标详情 使用细节 贮存及处理 图像
品牌 IHC-plus™
特异性 Human WNT9A. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except SCUBE2 (53 % ).
预测反应 Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey, Bat (94%) Mouse, Rat, Hamster, Bovine, Rabbit (88%) Elephant, Dog, Horse (82%).
纯化方法 Immunoaffinity purified
免疫原 Synthetic 17 amino acid peptide from internal region of human WNT9A. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey, Bat (94%), Mouse, Rat, Hamster, Bovine, Rabbit (88%), Elephant, Dog, Horse (82%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

WNT9A目标详情

WNT9A抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 WNT14 / WNT9A (WNT9A Antibody 摘要)
背景 Name/Gene ID: WNT9A
Family: WNT

Synonyms: WNT9A, Protein Wnt-14, Protein Wnt-9a, WNT14
基因ID 7483
途径 WNT signaling

使用细节

WNT9A抗体详情 WNT9A目标详情 贮存及处理 图像 回页首
应用备注 Approved: IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
说明

Target Species of Antibody: Human

实验流程 The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
限制 仅限研究用

贮存及处理

WNT9A抗体详情 WNT9A目标详情 使用细节 图像 回页首
状态 Liquid
浓度 Lot specific
缓冲液 PBS, less than 0.1 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C,-20 °C
储存方法 Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
有效期 12 months

图像

WNT9A抗体详情 WNT9A目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) (Internal Region) antibody (ABIN1049487) Anti-WNT9A antibody ABIN1049487 IHC staining of human adrenal. Immunohistochemistry ...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) (Internal Region) antibody (ABIN1049487) Anti-WNT9A antibody ABIN1049487 IHC staining of human colon. Immunohistochemistry of...
Immunohistochemistry (IHC) image for anti-Wingless-Type MMTV Integration Site Family, Member 9A (WNT9A) (Internal Region) antibody (ABIN1049487) Anti-WNT9A antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, par...