anti-小鼠 Rhodopsin 抗体 for Immunofluorescence

Recommended Rhodopsin Antibody (supplied by: Log in to see )

抗原
Rhodopsin (RHO) 抗体
  • CSNBAD1
  • OPN2
  • RP4
  • RH1
  • Rh
  • fi06d11
  • wu:fi06d11
  • zfo2
  • zfrho
  • Noerg1
  • Opn2
  • Ops
  • opn2
  • rh1
  • rp4
  • xrho
  • PIGRHO1
  • ops
  • RDP1
  • RHO
  • rhodopsin
  • rhodopsin L homeolog
  • RHO
  • Rho
  • rho
  • rho.L
适用
人, 牛, 小鼠, 猪, 大鼠
157
59
52
52
38
36
36
35
22
22
21
18
18
16
4
2
2
2
2
1
1
宿主
小鼠
238
74
5
1
克隆类型
单克隆
标记
This Rhodopsin antibody is un-conjugated
15
14
13
13
12
10
9
6
6
6
6
6
6
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
2
2
2
2
应用范围
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
294
218
179
163
152
134
59
40
3
2
1
1
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产品编号 ABIN1580458
$ 220.00
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2451717 IF IHC IHC (p) WB Mouse IgG1 Log in to see RET-P1 0
1 ABIN1960118 IF IHC IHC (p) WB Mouse IgG1, kappa Log in to see A531 0
1 ABIN1960117 ICC IF IHC IHC (p) WB Mouse IgG1, kappa Log in to see B630 0
1 ABIN220902 IF IHC IHC (p) WB Mouse IgG1 Log in to see 0
1 ABIN384468 IF IHC IHC (p) WB Biotin Mouse IgG1 Log in to see RET-P1 0
1 ABIN2964046 ICC IF IHC (p) WB Mouse IgG1 kappa Log in to see B630 0

General

抗原 Rhodopsin (RHO) 抗体
适用 人, 牛, 小鼠, 猪, 大鼠
(157), (59), (52), (52), (38), (36), (36), (35), (22), (22), (21), (18), (18), (16), (4), (2), (2), (2), (2), (1), (1)
宿主 小鼠
(238), (74), (5), (1)
克隆类型 (克隆位点)
单克隆   ( )
标记 This Rhodopsin antibody is un-conjugated
(15), (14), (13), (13), (12), (10), (9), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (2), (2), (2), (2)
应用范围 Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(294), (218), (179), (163), (152), (134), (59), (40), (3), (2), (1), (1)
Pubmed 2个引用可参考
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Rhodopsin抗体详情

Rhodopsin目标详情 使用细节 贮存及处理 References for anti-Rhodopsin antibody (ABIN1580458) 图像
纯化方法 affinity purified antibody
克隆位点 B630
亚型 IgG1
Plasmids, Primers & others

Rhodopsin目标详情

Rhodopsin抗体详情 使用细节 贮存及处理 References for anti-Rhodopsin antibody (ABIN1580458) 图像 回页首
抗原
别名 Rhodopsin (RHO Antibody 摘要)
背景 Rhodopsin is the protein in the mammalian retina responsible for the light sensitivity of rod cells, which are responsible for vision in low light levels. Somewhat surprisingly, the rhodopsin protein turned out to be a typical member of the seven transmembrane G protein-coupled receptor (GPCR) superfamily. Whereas other GPCRs initiate signaling on binding a specific ligand, rhodopsin exists with a ligand already bound, specifically the vitamin A related substance retinal. Retinal can exist in two isomeric forms, 11-cis and 11-trans retinal. In the dark rhodopsin is associated with 11-cis retinal, but photons cause the 11-cis form to flip to the 11-trans form, and this causes an alteration in the structure of the rhodopsin making it catalytically active. Activated rhodopsin in turn activates the GTP binding protein transducin by favoring the loss of GDP and the addition of GTP. Transducin is a typical member of the family of heterotrimeric G proteins, and consists of an alpha, and a beta,gamma, subunit. The alpha, subunit is responsible for the GTP binding and the GTP bound form activates a phosphodiesterase (PDE) enzyme which hydrolyses cyclic GMP. This in turn results in the closure of cyclic nucleotide gated ion channels in the rod cell membrane, which prevent the influx of sodium and calcium ions. This results in an increase in the membrane potential of the rod cell and so reduces the rate of synaptic signaling. So light stimulation results in a reduced rate of photoreceptor synaptic release. This information is transmitted through neurons of the retina to the visual centers of the brain (see review 1, 2). Rhodopsin activity is shut off by phosphorylation under the influence of rhodopsin kinase, the activity of which results in binding of visual arrestin, which prevents rhodopsin from interacting with and activating more transducin molecules. This basic signaling paradigm proved to be a prototype for understanding how other GPCRs function, as proteins similar to transducin, arrestin and rhodopsin kinase are found in these pathways. MCA-B630 was raised against bovine outer segment discs and shows convincing staining for rhodopsin both on western blots and on sections of retina. The protein is concentrated in rod outer segments. The HGNC name for this protein is RHO.
途径 WNT signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction

使用细节

Rhodopsin抗体详情 Rhodopsin目标详情 贮存及处理 References for anti-Rhodopsin antibody (ABIN1580458) 图像 回页首
应用备注 Try at dilutions of ~1:1,000 for immunofluorescence. For western blots try at 1:5,000. A suitable control tissue is retinal extracts. Rhodopsin run at 35 kDa on SDS-PAGE gels.
限制 仅限研究用

贮存及处理

Rhodopsin抗体详情 Rhodopsin目标详情 使用细节 References for anti-Rhodopsin antibody (ABIN1580458) 图像 回页首
状态 Liquid
浓度 1 mg/mL
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store at 4°C short term or -20°C long term.

References for anti-Rhodopsin antibody (ABIN1580458)

Rhodopsin抗体详情 Rhodopsin目标详情 使用细节 贮存及处理 图像 回页首
背景

Molday: "Photoreceptor membrane proteins, phototransduction, and retinal degenerative diseases. The Friedenwald Lecture." in: Investigative ophthalmology & visual science, Vol. 39, Issue 13, pp. 2491-513, 1998

Yau: "Phototransduction mechanism in retinal rods and cones. The Friedenwald Lecture." in: Investigative ophthalmology & visual science, Vol. 35, Issue 1, pp. 9-32, 1994

图像

Rhodopsin抗体详情 Rhodopsin目标详情 使用细节 贮存及处理 References for anti-Rhodopsin antibody (ABIN1580458) 回页首
厂商提供的图像
Western Blotting (WB) image for anti-Rhodopsin (RHO) antibody (ABIN1580458) Blot of bovine retinal extracts probed with ABIN1580458. The antibody stains a band c...
Immunofluorescence (IF) image for anti-Rhodopsin (RHO) antibody (ABIN1580458) High magnification confocal image of pig retinal section stained with ABIN1580458 (Gr...