anti-绵羊 PKC alpha 抗体 for Flow Cytometry

Recommended PKC alpha Antibody (supplied by: Log in to see )

抗原
Protein Kinase C, alpha (PKCa) 抗体
  • pkc-alpha
  • pkcalpha
  • im:7139045
  • si:dkey-8l19.1
  • LOC100219195
  • AAG6
  • PKC-alpha
  • PKCA
  • PRKACA
  • AI875142
  • Pkca
  • PKRCA
  • protein kinase C alpha
  • protein kinase C, alpha L homeolog
  • protein kinase C, alpha
  • PRKCA
  • prkca.L
  • prkca
  • Prkca
适用
牛, 犬, 鱼, 人, 小鼠, 猪, 兔, 大鼠, 绵羊
299
214
170
30
25
24
20
20
19
5
3
3
2
1
1
1
1
宿主
小鼠
267
45
2
克隆类型
单克隆
标记
This PKC alpha antibody is un-conjugated
14
13
10
7
6
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
应用范围
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
255
143
134
70
54
44
33
26
25
22
13
5
4
3
2
1
1
1
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产品编号 ABIN153146
产品在该地区无法提供。
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4345802 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB Mouse IgG2a Log in to see MC5 0
1 ABIN4345823 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG2a Log in to see MC5 0
1 ABIN4345818 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG2a Log in to see MC5 0
1 ABIN4345822 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG2a Log in to see MC5 0
1 ABIN4345824 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG2a Log in to see MC5 0
1 ABIN4345819 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG2a Log in to see MC5 0
1 ABIN4345821 ELISA FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG2a Log in to see MC5 0
1 ABIN4345811 ELISA FACS ICC IF IHC (fro) IHC (p) FITC Mouse IgG2a Log in to see MC5 0
1 ABIN4345816 FACS APC Mouse IgG2a Log in to see MC5 0
1 ABIN4345815 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP RIA WB PerCP Mouse IgG2a Log in to see MC5 0
1 ABIN4345817 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 755 Mouse IgG2a Log in to see MC5 0
1 ABIN4345812 FACS PE Mouse IgG2a Log in to see MC5 0
1 ABIN4345813 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgG2a Log in to see MC5 0
1 ABIN4345814 ELISA FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Mouse IgG2a Log in to see MC5 0
1 ABIN5599622 ELISA FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 650 Mouse IgG2a Log in to see MC5 0
1 ABIN5599621 ELISA FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 550 Mouse IgG2a Log in to see MC5 0

General

抗原 Protein Kinase C, alpha (PKCa) 抗体
适用 牛, 犬, 鱼, 人, 小鼠, 猪, 兔, 大鼠, 绵羊
(299), (214), (170), (30), (25), (24), (20), (20), (19), (5), (3), (3), (2), (1), (1), (1), (1)
宿主 小鼠
(267), (45), (2)
克隆类型 (克隆位点)
单克隆   ( )
标记 This PKC alpha antibody is un-conjugated
(14), (13), (10), (7), (6), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
(255), (143), (134), (70), (54), (44), (33), (26), (25), (22), (13), (5), (4), (3), (2), (1), (1), (1)
Pubmed 14个引用可参考
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PKC alpha抗体详情

PKC alpha目标详情 使用细节 贮存及处理 References for anti-PKC alpha antibody (ABIN153146) 图像
特异性 This antibody is capable of detecting PKC beta as well.
纯化方法 Protein A purified
免疫原 Purified bovine brain Protein Kinase C
克隆位点 MC5
亚型 IgG2a
Plasmids, Primers & others

PKC alpha目标详情

PKC alpha抗体详情 使用细节 贮存及处理 References for anti-PKC alpha antibody (ABIN153146) 图像 回页首
抗原
别名 PKC alpha (PKCa Antibody 摘要)
背景 Gene Symbol: PRKCA
基因ID 5578
UniProt P17252, P04409
途径 WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling

使用细节

PKC alpha抗体详情 PKC alpha目标详情 贮存及处理 References for anti-PKC alpha antibody (ABIN153146) 图像 回页首
应用备注 Western Blot 1:1000, Flow Cytometry 1:10-1:1000, ELISA 1:100-1:2000, Immunohistochemistry 1:200-1:500, Immunocytochemistry/Immunofluorescence 1:100-1:500, Immunoprecipitation 1:10-1:100, Immunohistochemistry-Paraffin 1:200-1:500, Immunohistochemistry-Frozen 1:200-1:500, RadioimmunoassayThis PKC alpha Antibody (MC5) is useful for Western blot, ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation and Radioimmunoassay. In WB a band can be seen at ~75 kDa representing PKC alpha.
说明

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

限制 仅限研究用

贮存及处理

PKC alpha抗体详情 PKC alpha目标详情 使用细节 References for anti-PKC alpha antibody (ABIN153146) 图像 回页首
状态 Liquid
浓度 1.0 mg/mL
缓冲液 Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid freeze-thaw cycles
储存条件 4 °C,-20 °C
储存方法 Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-PKC alpha antibody (ABIN153146)

PKC alpha抗体详情 PKC alpha目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Xie, Huo, Li, Dai, Xu, Yin: "Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the Müller Cell Notch Signaling Pathway." in: Cell transplantation, Vol. 26, Issue 6, pp. 967-982, 2018 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Rat (Rattus)).

Guimarães-Souza, Perche, Morgans, Duvoisin, Calaza: "Fragile X Mental Retardation Protein expression in the retina is regulated by light." in: Experimental eye research, Vol. 146, pp. 72-82, 2016

Zimov, Yazulla: "Novel processes invaginate the pre-synaptic terminal of retinal bipolar cells." in: Cell and tissue research, Vol. 333, Issue 1, pp. 1-16, 2008

Quraishi, Gayet, Morgans, Duvoisin: "Distribution of group-III metabotropic glutamate receptors in the retina." in: The Journal of comparative neurology, Vol. 501, Issue 6, pp. 931-43, 2007

Bayley, Morgans: "Rod bipolar cells and horizontal cells form displaced synaptic contacts with rods in the outer nuclear layer of the nob2 retina." in: The Journal of comparative neurology, Vol. 500, Issue 2, pp. 286-98, 2006

Ng, Parsons, Hughes, Monypenny, Zicha, Gautreau, Arpin, Gschmeissner, Verveer, Bastiaens, Parker: "Ezrin is a downstream effector of trafficking PKC-integrin complexes involved in the control of cell motility." in: The EMBO journal, Vol. 20, Issue 11, pp. 2723-41, 2001 (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunoprecipitation,Immunofluorescence

Cesare, Dekker, Sardini, Parker, McNaughton: "Specific involvement of PKC-epsilon in sensitization of the neuronal response to painful heat." in: Neuron, Vol. 23, Issue 3, pp. 617-24, 1999 (Sample species: Rat (Rattus)). Further details: Western Blotting

Ricciarelli, Tasinato, Clément, Ozer, Boscoboinik, Azzi: "alpha-Tocopherol specifically inactivates cellular protein kinase C alpha by changing its phosphorylation state." in: The Biochemical journal, Vol. 334 ( Pt 1), pp. 243-9, 1998 (Sample species: Rat (Rattus)). Further details: Western Blotting

Gatti, Robinson: "Unique phosphorylation of protein kinase C-alpha in PC12 cells induces resistance to translocation and down-regulation." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31718-22, 1997 (Sample species: Rat (Rattus)). Further details: Western Blotting

Clément, Tasinato, Boscoboinik, Azzi: "The effect of alpha-tocopherol on the synthesis, phosphorylation and activity of protein kinase C in smooth muscle cells after phorbol 12-myristate 13-acetate down-regulation." in: European journal of biochemistry, Vol. 246, Issue 3, pp. 745-9, 1997 (Sample species: Rat (Rattus)). Further details: Western Blotting

Gatti, Robinson: "Okadaic acid interferes with phorbol-ester-mediated down-regulation of protein kinase C-alpha, C-delta and C-epsilon." in: European journal of biochemistry, Vol. 249, Issue 1, pp. 92-7, 1997 (Sample species: Rat (Rattus)). Further details: Western Blotting

Wyss-Coray, Masliah, Toggas, Rockenstein, Brooker, Lee, Mucke: "Dysregulation of signal transduction pathways as a potential mechanism of nervous system alterations in HIV-1 gp120 transgenic mice and humans with HIV-1 encephalitis." in: The Journal of clinical investigation, Vol. 97, Issue 3, pp. 789-98, 1996 Further details: Immunocytochemistry,Immunofluorescence

Escribá, Sastre, García-Sevilla: "Disruption of cellular signaling pathways by daunomycin through destabilization of nonlamellar membrane structures." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 16, pp. 7595-9, 1995 (Sample species: Rat (Rattus)). Further details: Western Blotting

Lacal, Cuadrado, Jones, Trotta, Burstein, Thomson, Pellicer: "Regulation of protein kinase C activity in neuronal differentiation induced by the N-ras oncogene in PC-12 cells." in: Molecular and cellular biology, Vol. 10, Issue 6, pp. 2983-90, 1990 (Sample species: Human). Further details: Western Blotting

图像

PKC alpha抗体详情 PKC alpha目标详情 使用细节 贮存及处理 References for anti-PKC alpha antibody (ABIN153146) 回页首
厂商提供的图像
Immunofluorescence (IF) image for anti-Protein Kinase C, alpha (PKCa) antibody (ABIN153146) Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (MC5) [ABIN153146] - PKC a...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Protein Kinase C, alpha (PKCa) antibody (ABIN153146) Immunohistochemistry-Paraffin: PKC alpha Antibody (MC5) [ABIN153146] - IHC staining o...