anti-牛 CAMK2G 抗体 for Immunoprecipitation

Recommended CAMK2G Antibody (supplied by: Log in to see )

抗原
Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) 抗体
  • CAMK
  • CAMK-II
  • CAMKG
  • 5930429P18Rik
  • Camkg
  • calcium/calmodulin dependent protein kinase II gamma
  • calcium/calmodulin-dependent protein kinase II gamma
  • CAMK2G
  • Camk2g
适用
牛, 人, 小鼠, 大鼠
145
114
112
38
2
2
2
2
1
1
1
1
1
宿主
小鼠
88
69
克隆类型
单克隆
标记
This CAMK2G antibody is un-conjugated
5
5
5
5
5
5
4
4
4
4
4
4
4
4
4
4
4
应用范围
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
154
105
98
78
52
40
40
36
8
4
1
1
1
1
1
选项
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产品编号 ABIN361644
$ 349.80
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2484549 ICC IF IP RIA IHC ELISA WB Atto 390 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484552 ICC IF IP RIA IHC ELISA WB Atto 594 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484553 ICC IF IP RIA IHC ELISA WB Atto 633 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484556 ICC IF IP RIA IHC ELISA WB Atto 700 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484560 ICC IF IP RIA IHC ELISA WB FITC Mouse IgG1 Log in to see 6G9 2
1 ABIN2484559 ICC IF IP RIA IHC ELISA WB Biotin Mouse IgG1 Log in to see 6G9 2
1 ABIN2484563 ICC IF IP RIA IHC ELISA WB PerCP Mouse IgG1 Log in to see 6G9 2
1 ABIN2484561 ICC IF IP RIA IHC ELISA WB HRP Mouse IgG1 Log in to see 6G9 2
1 ABIN2484554 ICC IF IP RIA IHC ELISA WB Atto 655 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484555 ICC IF IP RIA IHC ELISA WB Atto 680 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484565 ICC IF IP RIA IHC ELISA WB Streptavidin Mouse IgG1 Log in to see 6G9 2
1 ABIN2484550 ICC IF IP RIA IHC ELISA WB Atto 488 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484551 ICC IF IP RIA IHC ELISA WB Atto 565 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484557 ICC IF IP RIA IHC ELISA WB Alkaline Phosphatase (AP) Mouse IgG1 Log in to see 6G9 2
1 ABIN2484558 ICC IF IP RIA IHC ELISA WB APC Mouse IgG1 Log in to see 6G9 2
1 ABIN2484562 ICC IF IP RIA IHC ELISA WB PE-Atto 594 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484564 ICC IF IP RIA IHC ELISA WB PE Mouse IgG1 Log in to see 6G9 2
1 ABIN5896021 ELISA IF/ICC IHC IP RIA WB PerCP Mouse IgG1 Log in to see 0
1 ABIN1721474 ICC IF IHC IP RIA WB Mouse IgG1 Log in to see 6G9 0
1 ABIN5896008 ELISA IF/ICC IHC IP RIA WB Atto 488 Mouse IgG1 Log in to see 0

General

抗原 Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) 抗体
适用 牛, 人, 小鼠, 大鼠
(145), (114), (112), (38), (2), (2), (2), (2), (1), (1), (1), (1), (1)
宿主 小鼠
(88), (69)
克隆类型 (克隆位点)
单克隆   ( )
标记 This CAMK2G antibody is un-conjugated
(5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4)
应用范围 Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
(154), (105), (98), (78), (52), (40), (40), (36), (8), (4), (1), (1), (1), (1), (1)
Pubmed 13个引用可参考
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CAMK2G抗体详情

CAMK2G目标详情 使用细节 贮存及处理 References for anti-CAMK2G antibody (ABIN361644) 图像
特异性 Detects ~50-60 kDa. Recognizes both phosphorylated and non-phosphorylated forms.
纯化方法 Protein G Purified
免疫原 Partially purified rat CaMKII
克隆位点 6G9
亚型 IgG1
Plasmids, Primers & others

CAMK2G目标详情

CAMK2G抗体详情 使用细节 贮存及处理 References for anti-CAMK2G antibody (ABIN361644) 图像 回页首
抗原
别名 CaMKII (CAMK2G Antibody 摘要)
背景 CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
基因ID 12322
NCBI登录号 NP_033922
UniProt P11798
途径 WNT signaling, Interferon-gamma Pathway, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of long-term Neuronal Synaptic Plasticity

使用细节

CAMK2G抗体详情 CAMK2G目标详情 贮存及处理 References for anti-CAMK2G antibody (ABIN361644) 图像 回页首
应用备注
  • WB (1:10000)
  • IHC (1:2000)
  • ICC/IF (1:50)
  • optimal dilutions for assays should be determined by the user.
说明

0.1 μg/ml was sufficient for detection of CamKII in 20 μg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.

限制 仅限研究用

贮存及处理

CAMK2G抗体详情 CAMK2G目标详情 使用细节 References for anti-CAMK2G antibody (ABIN361644) 图像 回页首
状态 Liquid
浓度 1 mg/mL
缓冲液 PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C

References for anti-CAMK2G antibody (ABIN361644)

CAMK2G抗体详情 CAMK2G目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Feng, Szabo, Dziak, Opas: "Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells." in: Stem cell reviews, Vol. 6, Issue 1, pp. 74-85, 2010

背景

Hughes, Edin, Antonsson, Grundström: "Calmodulin-dependent kinase II mediates T cell receptor/CD3- and phorbol ester-induced activation of IkappaB kinase." in: The Journal of biological chemistry, Vol. 276, Issue 38, pp. 36008-13, 2001

Means: "Regulatory cascades involving calmodulin-dependent protein kinases." in: Molecular endocrinology (Baltimore, Md.), Vol. 14, Issue 1, pp. 4-13, 2000

Leonard, Lim, Hemsworth, Horne, Hell: "Calcium/calmodulin-dependent protein kinase II is associated with the N-methyl-D-aspartate receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 6, pp. 3239-44, 1999

Makhinson, Chotiner, Watson, ODell: "Adenylyl cyclase activation modulates activity-dependent changes in synaptic strength and Ca2+/calmodulin-dependent kinase II autophosphorylation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 7, pp. 2500-10, 1999

Shen, Meyer: "Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation." in: Science (New York, N.Y.), Vol. 284, Issue 5411, pp. 162-6, 1999

Strack, Colbran: "Autophosphorylation-dependent targeting of calcium/ calmodulin-dependent protein kinase II by the NR2B subunit of the N-methyl- D-aspartate receptor." in: The Journal of biological chemistry, Vol. 273, Issue 33, pp. 20689-92, 1998

Barria, Muller, Derkach, Griffith, Soderling: "Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation." in: Science (New York, N.Y.), Vol. 276, Issue 5321, pp. 2042-5, 1997

Tombes, Krystal: "Identification of novel human tumor cell-specific CaMK-II variants." in: Biochimica et biophysica acta, Vol. 1355, Issue 3, pp. 281-92, 1997

Brocke, Srinivasan, Schulman: "Developmental and regional expression of multifunctional Ca2+/calmodulin-dependent protein kinase isoforms in rat brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 10, pp. 6797-808, 1995

Edman, Schulman: "Identification and characterization of delta B-CaM kinase and delta C-CaM kinase from rat heart, two new multifunctional Ca2+/calmodulin-dependent protein kinase isoforms." in: Biochimica et biophysica acta, Vol. 1221, Issue 1, pp. 89-101, 1994

Nghiem, Saati, Martens, Gardner, Schulman: "Cloning and analysis of two new isoforms of multifunctional Ca2+/calmodulin-dependent protein kinase. Expression in multiple human tissues." in: The Journal of biological chemistry, Vol. 268, Issue 8, pp. 5471-9, 1993

Bennett, Kennedy: "Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 7, pp. 1794-8, 1987

图像

CAMK2G抗体详情 CAMK2G目标详情 使用细节 贮存及处理 References for anti-CAMK2G antibody (ABIN361644) 回页首
厂商提供的图像
 image for anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (ABIN361644) CamKII (6G9) in cultured cortical neurons.
Western Blotting (WB) image for anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (ABIN361644) western rat membrane 1 in 1000 CaMKII.