anti-人 TLR9 抗体 for Enzyme Immunoassay

Recommended TLR9 Antibody (supplied by: Log in to see )

抗原
Toll-Like Receptor 9 (TLR9) 抗体
  • CD289
  • toll like receptor 9
  • toll-like receptor 9
  • TLR9
  • Tlr9
抗原表位
AA 1-815, Extracellular Domain, N-Term
39
31
14
13
12
11
8
7
7
6
6
6
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
适用
犬, 人, 小鼠
261
147
55
35
23
20
7
5
3
2
2
2
2
2
1
1
宿主
小鼠
143
123
35
14
4
3
克隆类型
单克隆
标记
This TLR9 antibody is conjugated to FITC
27
24
17
8
6
6
5
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
227
163
132
109
88
39
36
36
14
7
6
6
5
5
5
3
3
3
2
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产品编号 ABIN1106226
$ 489.50
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1106225 EIA IHC (fro) IF IHC (p) WB Biotin Mouse IgG2a AA 1-815, Extracellular Domain, N-Term Log in to see 5G5 7
1 ABIN5555342 EIA IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN5555343 EIA IF WB Rabbit IgG Center Log in to see Polyclonal 0
1 ABIN784145 EIA IF IHC (p) WB Goat IgG AA 204-224, N-Term Log in to see Polyclonal 0
1 ABIN187334 EIA FACS IHC (fro) IHC (p) WB Mouse IgG2a Log in to see 5G5 0

General

抗原 Toll-Like Receptor 9 (TLR9) 抗体
抗原表位 AA 1-815, Extracellular Domain, N-Term
(39), (31), (14), (13), (12), (11), (8), (7), (7), (6), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用 犬, 人, 小鼠
(261), (147), (55), (35), (23), (20), (7), (5), (3), (2), (2), (2), (2), (2), (1), (1)
宿主 小鼠
(143), (123), (35), (14), (4), (3)
克隆类型 (克隆位点)
单克隆   ( )
标记 This TLR9 antibody is conjugated to FITC
(27), (24), (17), (8), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
应用范围 Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(227), (163), (132), (109), (88), (39), (36), (36), (14), (7), (6), (6), (5), (5), (5), (3), (3), (3), (2)
Pubmed 7个引用可参考
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TLR9抗体详情

TLR9目标详情 使用细节 贮存及处理 References for anti-TLR9 antibody (ABIN1106226) 图像
特异性 The monoclonal antibody 5G5 recognizes human Toll-like receptor 9.
交叉反应 (详细) Species reactivity (tested):Human, mouse, canine
纯化方法 Protein G
免疫原 Purified fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc
克隆位点 5G5
亚型 IgG2a
Plasmids, Primers & others

TLR9目标详情

TLR9抗体详情 使用细节 贮存及处理 References for anti-TLR9 antibody (ABIN1106226) 图像 回页首
抗原
别名 CD289 / TLR9 (TLR9 Antibody 摘要)
背景 Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called 'pathogen-associated molecular patterns'. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NFκB-dependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens. Whereas most TLRs are expressed on the cell surface, TLR9 is expressed intracellularly within one or more endosomal compartments and recognizes nucleic acids. TLR9 detects a rather subtle difference in the DNA of vertebrates compared with that of pathogens. Vertebrate genomic DNAs have mostly methylated CpG dinucleotides where bacterial and viral DNAs have unmethylated CpG dinucleotides. TLR9 undergoes relocation from endoplasmic reticulum to CpG-ODN-containing endosomes. In these endosomes TLR9 becomes a functional receptor after proteolytic cleavage. TLR9 exists as a preformed homodimer and CpG-ODN binding promotes its conformational change, bringing the cytoplasmic TIR-like domains close to each other. This allows a recruitment of the key adapter protein MyD88 which initiates a signalling cascade. The only human immune cell types known to constitutively express TLR9 and to be activated by CpG ODN are pDCs and B cells. TLR9 triggering induces an activation phenotype in the B cells and pDCs, characterized by the expression of costimulatory molecules, resistance to apoptosis, and induces Th1-type immune response profiles.Synonyms: Toll-like receptor 9, UNQ5798/PRO19605
基因ID 81897
NCBI登录号 NP_112455
UniProt Q9EQU3
途径 TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades

使用细节

TLR9抗体详情 TLR9目标详情 贮存及处理 References for anti-TLR9 antibody (ABIN1106226) 图像 回页首
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用

贮存及处理

TLR9抗体详情 TLR9目标详情 使用细节 References for anti-TLR9 antibody (ABIN1106226) 图像 回页首
浓度 0.1 mg/mL
缓冲液 PBS, 1 % bovine serum albumin, 0.02 % sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store at 2 - 8 °C.

References for anti-TLR9 antibody (ABIN1106226)

TLR9抗体详情 TLR9目标详情 使用细节 贮存及处理 图像 回页首
背景

Jeong, Choi, Cho, Lee, Oh, Park, Cho, Lee, Kim, Han, Choi, Chung: "Chronic gastrointestinal symptoms and quality of life in the Korean population." in: World journal of gastroenterology : WJG, Vol. 14, Issue 41, pp. 6388-94, 2008

Burgener, Jungi: "Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry." in: Veterinary immunology and immunopathology, Vol. 124, Issue 1-2, pp. 184-91, 2008

Tokumasa, Suto, Kagami, Furuta, Hirose, Watanabe, Saito, Shimoda, Iwamoto, Nakajima: "Expression of Tyk2 in dendritic cells is required for IL-12, IL-23, and IFN-gamma production and the induction of Th1 cell differentiation." in: Blood, Vol. 110, Issue 2, pp. 553-60, 2007

Pratesi, Petrangolini, Tortoreto, Addis, Belluco, Rossini, Selleri, Rumio, Menard, Balsari: "Therapeutic synergism of gemcitabine and CpG-oligodeoxynucleotides in an orthotopic human pancreatic carcinoma xenograft." in: Cancer research, Vol. 65, Issue 14, pp. 6388-93, 2005

Rutz, Metzger, Gellert, Luppa, Lipford, Wagner, Bauer: "Toll-like receptor 9 binds single-stranded CpG-DNA in a sequence- and pH-dependent manner." in: European journal of immunology, Vol. 34, Issue 9, pp. 2541-50, 2004

Rumio, Besusso, Palazzo, Selleri, Sfondrini, Dubini, Ménard, Balsari: "Degranulation of paneth cells via toll-like receptor 9." in: The American journal of pathology, Vol. 165, Issue 2, pp. 373-81, 2004

Ahmad-Nejad, Häcker, Rutz, Bauer, Vabulas, Wagner: "Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments." in: European journal of immunology, Vol. 32, Issue 7, pp. 1958-68, 2002

图像

TLR9抗体详情 TLR9目标详情 使用细节 贮存及处理 References for anti-TLR9 antibody (ABIN1106226) 回页首
厂商提供的图像
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 9 (TLR9) (AA 1-815), (Extracellular Domain), (N-Term) antibody (FITC) (ABIN1106226) anti-Toll-Like Receptor 9 (TLR9) (AA 1-815), (Extracellular Domain), (N-Term) antibody (FITC)