anti-人 Heat Shock Protein 90kDa beta (Grp94), Member 1 抗体 for Western Blotting

Recommended Heat Shock Protein 90kDa beta (Grp94), Member 1 Antibody (supplied by: Log in to see )

抗原
Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) 抗体
  • ecgp
  • gp96
  • tra1
  • grp94
  • DDBDRAFT_0206450
  • DDBDRAFT_0215015
  • DDB_0206450
  • DDB_0215015
  • TRA1
  • HSP90B1
  • ERp99
  • GRP94
  • TA-3
  • Targ2
  • Tra-1
  • Tra1
  • endoplasmin
  • GP96
  • fb61d09
  • tra-1
  • wu:fb61d09
  • wu:fq25g01
  • Grp94
  • ECGP
  • Gp96
  • ppk98
  • hsp108
  • heat shock protein 90kDa beta (Grp94), member 1
  • heat shock protein Hsp90 family protein
  • 94 kD glucose-regulated protein
  • heat shock protein 90 beta family member 1
  • heat shock protein 90, beta (Grp94), member 1
  • heat shock protein 90, beta (grp94), member 1
  • heat shock protein 90kDa beta family member 1 L homeolog
  • hsp90b1
  • grp94
  • HSP90B1
  • Hsp90b1
  • hsp90b1.L
抗原表位
C-Term
75
33
20
15
12
12
12
11
9
8
7
5
5
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
适用
牛, 人, 小鼠, 大鼠
415
283
228
158
107
106
97
97
96
96
93
91
91
77
18
8
3
3
2
2
1
1
宿主

253
133
47
18
2
克隆类型
多克隆
标记
Biotin
20
18
17
16
15
15
10
8
8
8
8
8
8
8
8
8
5
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
418
201
170
163
158
97
97
85
44
43
13
8
8
3
选项
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

产品编号 ABIN2481967
$ 398.20
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0807028 ABIN2481972 ICC IF IP IHC WB PE Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2481960 ICC IF IP IHC WB Atto 594 Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2481966 ICC IF IP IHC WB APC Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2481970 ICC IF IP IHC WB PE-Atto 594 Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2481973 ICC IF IP IHC WB Streptavidin Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2481961 ICC IF IP IHC WB Atto 633 Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2481959 ICC IF IP IHC WB Atto 565 Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2481962 ICC IF IP IHC WB Atto 655 Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2481964 ICC IF IP IHC WB Atto 700 Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2481965 ICC IF IP IHC WB Alkaline Phosphatase (AP) Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2481963 ICC IF IP IHC WB Atto 680 Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2481968 ICC IF IP IHC WB FITC Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2481958 ICC IF IP IHC WB Atto 488 Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2481969 ICC IF IP IHC WB HRP Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2481971 ICC IF IP IHC WB PerCP Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2481957 ICC IF IP IHC WB Atto 390 Rabbit C-Term Log in to see Polyclonal 13
3.0807028 ABIN2778847 IHC WB Rabbit C-Term Log in to see Polyclonal 1
3.0807028 ABIN2855578 ICC IF IP IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
3.0807028 ABIN2855586 ICC IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
3.0807028 ABIN152772 ICC IF IHC (p) IP IHC WB Rat IgG2a Log in to see 9G10 0

General

抗原 Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) 抗体
抗原表位 C-Term
(75), (33), (20), (15), (12), (12), (12), (11), (9), (8), (7), (5), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
适用 牛, 人, 小鼠, 大鼠
(415), (283), (228), (158), (107), (106), (97), (97), (96), (96), (93), (91), (91), (77), (18), (8), (3), (3), (2), (2), (1), (1)
宿主
(253), (133), (47), (18), (2)
克隆类型
多克隆
标记 Biotin
(20), (18), (17), (16), (15), (15), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(418), (201), (170), (163), (158), (97), (97), (85), (44), (43), (13), (8), (8), (3)
Pubmed 13个引用可参考
Supplier Log in to see

产品细节

目标详细情况 使用细节 贮存及处理 引用 图像
特异性 Detects ~94 kDa.
纯化方法 Peptide Affinity Purified
免疫原 Synthetic peptide corresponding to the sequence near the C-terminus of mouse GRP94

目标详细情况

产品细节 使用细节 贮存及处理 引用 图像 回页首
抗原
别名 GRP94 (HSP90B1 Antibody 摘要)
背景 Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50 % sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).
基因ID 22027
NCBI登录号 NP_035761
UniProt P08113
途径 Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades

使用细节

产品细节 目标详细情况 贮存及处理 引用 图像 回页首
应用备注
  • WB (1:1000)
  • ICC/IF (1:120)
  • IP (1:80)
  • optimal dilutions for assays should be determined by the user.
说明

1 μg/ml of SPC-101 was sufficient for detection of Grp94 in 20 μg of Hela lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

限制 仅限研究用

贮存及处理

产品细节 目标详细情况 使用细节 引用 图像 回页首
状态 Liquid
浓度 1 mg/mL
缓冲液 PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C

引用

产品细节 目标详细情况 使用细节 贮存及处理 图像 回页首
背景

Chu, Maynard, Chiosis, Nicchitta, Burlingame: "Identification of novel quaternary domain interactions in the Hsp90 chaperone, GRP94." in: Protein science : a publication of the Protein Society, Vol. 15, Issue 6, pp. 1260-9, 2006

Soldano, Jivan, Nicchitta, Gewirth: "Structure of the N-terminal domain of GRP94. Basis for ligand specificity and regulation." in: The Journal of biological chemistry, Vol. 278, Issue 48, pp. 48330-8, 2003

Sato, Torimoto, Tamura, Shindo, Shinzaki, Hirai, Kohgo: "Immunotherapy using heat-shock protein preparations of leukemia cells after syngeneic bone marrow transplantation in mice." in: Blood, Vol. 98, Issue 6, pp. 1852-7, 2001

Gusarova, Caplan, Brodsky, Fisher: "Apoprotein B degradation is promoted by the molecular chaperones hsp90 and hsp70." in: The Journal of biological chemistry, Vol. 276, Issue 27, pp. 24891-900, 2001

Yun, Gärtner, Arendt, Hoyer: "Increase in vulnerability of middle-aged rat brain to lead by cerebral energy depletion." in: Brain research bulletin, Vol. 52, Issue 5, pp. 371-8, 2000

Riera, Roher, Miró, Gil, Trujillo, Aguilera, Plana, Itarte: "Association of protein kinase CK2 with eukaryotic translation initiation factor eIF-2 and with grp94/endoplasmin." in: Molecular and cellular biochemistry, Vol. 191, Issue 1-2, pp. 97-104, 1999

Choukhi, Ung, Wychowski, Dubuisson: "Involvement of endoplasmic reticulum chaperones in the folding of hepatitis C virus glycoproteins." in: Journal of virology, Vol. 72, Issue 5, pp. 3851-8, 1998

Hoshino, Wang, Devetten, Iwata, Kajigaya, Wise, Liu, Youssoufian: "Molecular chaperone GRP94 binds to the Fanconi anemia group C protein and regulates its intracellular expression." in: Blood, Vol. 91, Issue 11, pp. 4379-86, 1998

Ruddon, Bedows: "Assisted protein folding." in: The Journal of biological chemistry, Vol. 272, Issue 6, pp. 3125-8, 1997

Srivastava, Udono, Blachere, Li: "Heat shock proteins transfer peptides during antigen processing and CTL priming." in: Immunogenetics, Vol. 39, Issue 2, pp. 93-8, 1994

Peter, Nguyen Van, Söling: "Different sorting of Lys-Asp-Glu-Leu proteins in rat liver." in: The Journal of biological chemistry, Vol. 267, Issue 15, pp. 10631-7, 1992

Kang, Welch: "Characterization and purification of the 94-kDa glucose-regulated protein." in: The Journal of biological chemistry, Vol. 266, Issue 9, pp. 5643-9, 1991

Mazzarella, Green: "ERp99, an abundant, conserved glycoprotein of the endoplasmic reticulum, is homologous to the 90-kDa heat shock protein (hsp90) and the 94-kDa glucose regulated protein (GRP94)." in: The Journal of biological chemistry, Vol. 262, Issue 18, pp. 8875-83, 1987

图像

产品细节 目标详细情况 使用细节 贮存及处理 引用 回页首
厂商提供的图像
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibody (Biotin) (ABIN2481967) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP94 Polyclonal An...
Western Blotting (WB) image for anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibody (Biotin) (ABIN2481967) Western blot analysis of Rat brain cell lysates showing detection of ~ 94-100 kDa GRP...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibody (Biotin) (ABIN2481967) Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP94 Polyclonal An...