anti-小鼠 ITK 抗体 for Western Blotting

Recommended ITK Antibody (supplied by: Log in to see )

抗原
IL2-Inducible T-Cell Kinase (ITK) 抗体
  • Emt
  • Tcsk
  • Tsk
  • EMT
  • LPFS1
  • LYK
  • PSCTK2
  • IL2 inducible T cell kinase
  • IL2 inducible T-cell kinase
  • IL2-inducible T-cell kinase
  • Itk
  • ITK
抗原表位
pTyr512
23
18
17
14
9
7
5
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
适用
人, 小鼠
119
27
25
3
2
宿主

76
35
23
克隆类型
多克隆
标记
This ITK antibody is un-conjugated
8
7
6
6
6
6
应用范围
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
119
85
16
10
8
5
5
5
4
3
1
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产品编号 ABIN6271310
$ 450.00
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7.819988 ABIN1868785 ICC IHC WB Rabbit IgG AA 368-625 Log in to see Polyclonal 0
7.819988 ABIN2934403 IF/ICC IHC IP WB Rabbit AA 368-625 Log in to see Polyclonal 0
4.819988 ABIN5518767 WB Rabbit IgG AA 575-617, C-Term Log in to see Polyclonal 0
4.819988 ABIN1873325 WB Rabbit IgG Log in to see Polyclonal 0
4.819988 ABIN5928223 WB Rabbit IgG Log in to see Polyclonal 0
4.819988 ABIN5928221 WB Rabbit pTyr512 Log in to see Polyclonal 0
4.819988 ABIN2563488 ELISA WB Goat IgG C-Term Log in to see Polyclonal 0
1 ABIN5552654 WB Goat C-Term Log in to see Polyclonal 0
1 ABIN680653 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2746783 ELISA IHC IP WB FITC Rabbit IgG Log in to see Polyclonal 0
1 ABIN2746782 ELISA IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2624369 WB Rabbit AA 156-190 Log in to see Polyclonal 0
1 ABIN1906120 WB Rabbit AA 1-50 Log in to see Polyclonal 0
1 ABIN6101383 ELISA IHC WB Rabbit IgG pTyr512 Log in to see Polyclonal 0
1 ABIN5703865 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2934404 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1087337 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5647872 WB Rabbit IgG AA 575-617 Log in to see Polyclonal 0
1 ABIN6243872 WB Rabbit Ig Fraction AA 156-190, N-Term Log in to see Polyclonal 0
1 ABIN6055997 IF/ICC IHC WB Biotin Rabbit IgG Log in to see Polyclonal 0

General

抗原 IL2-Inducible T-Cell Kinase (ITK) 抗体
抗原表位 pTyr512
(23), (18), (17), (14), (9), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
适用 人, 小鼠
(119), (27), (25), (3), (2)
宿主
(76), (35), (23)
克隆类型
多克隆
标记 This ITK antibody is un-conjugated
(8), (7), (6), (6), (6), (6)
应用范围 ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(119), (85), (16), (10), (8), (5), (5), (5), (4), (3), (1)
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ITK抗体详情

ITK目标详情 使用细节 贮存及处理 图像
特异性 ITK (Phospho-Tyr512) Antibody detects endogenous levels of ITK only when phosphorylated at Tyr512
交叉反应 人, 小鼠
纯化方法 The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
免疫原 A synthesized peptide derived from human ITK (Phospho-Tyr512)
亚型 IgG
Plasmids, Primers & others

ITK目标详情

ITK抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 ITK (ITK Antibody 摘要)
背景

Description: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation (PubMed:12186560, PubMed:12682224, PubMed:21725281). Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3 (By similarity).

Gene: ITK

分子量 68kDa
基因ID 3702
UniProt Q08881
途径 TCR Signaling, Fc-epsilon Receptor Signaling Pathway

使用细节

ITK抗体详情 ITK目标详情 贮存及处理 图像 回页首
应用备注 WB 1:1000-3000, IHC 1:50-1:200
限制 仅限研究用

贮存及处理

ITK抗体详情 ITK目标详情 使用细节 图像 回页首
状态 Liquid
浓度 1 mg/mL
缓冲液 Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store at -20 °C.Stable for 12 months from date of receipt
有效期 12 months

图像

ITK抗体详情 ITK目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Immunohistochemistry (IHC) image for anti-IL2-Inducible T-Cell Kinase (ITK) (pTyr512) antibody (ABIN6271310) ABIN6271311 at 1/200 staining Human kidney tissue sections by IHC-P. The tissue was f...
Immunohistochemistry (IHC) image for anti-IL2-Inducible T-Cell Kinase (ITK) (pTyr512) antibody (ABIN6271310) ABIN6271311 at 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was f...
Immunohistochemistry (IHC) image for anti-IL2-Inducible T-Cell Kinase (ITK) (pTyr512) antibody (ABIN6271310) ABIN6271311 at 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was for...
Western Blotting (WB) image for anti-IL2-Inducible T-Cell Kinase (ITK) (pTyr512) antibody (ABIN6271310) Western blot analysis ITK (Phospho-Tyr512) using UV treated 293 whole cell lysates