anti-小鼠 ITK 抗体 for Western Blotting

Recommended ITK Antibody (supplied by: Log in to see )

抗原
IL2-Inducible T-Cell Kinase (ITK) 抗体
  • Emt
  • Tcsk
  • Tsk
  • EMT
  • LPFS1
  • LYK
  • PSCTK2
  • IL2 inducible T cell kinase
  • IL2 inducible T-cell kinase
  • IL2-inducible T-cell kinase
  • Itk
  • ITK
抗原表位
AA 1-26
24
17
14
10
9
9
7
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
适用
人, 小鼠
113
26
19
4
2
1
1
1
宿主
小鼠
65
33
27
克隆类型
单克隆
标记
This ITK antibody is un-conjugated
7
7
6
6
6
6
应用范围
Immunoprecipitation (IP), Western Blotting (WB)
111
82
9
9
9
9
8
5
3
2
1
选项
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产品编号 ABIN967287
产品在该地区无法提供。
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2596397 ABIN1873325 WB Rabbit IgG Log in to see Polyclonal 0
3.2596397 ABIN1868785 ICC IHC WB Rabbit IgG AA 368-625 Log in to see Polyclonal 0
3.2596397 ABIN2563488 ELISA WB Goat IgG C-Term Log in to see Polyclonal 0
1 ABIN5518767 WB Rabbit IgG AA 575-617, C-Term Log in to see Polyclonal 0
1 ABIN5552654 WB Goat C-Term Log in to see Polyclonal 0
1 ABIN680653 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2746783 ELISA IHC IP WB FITC Rabbit IgG Log in to see Polyclonal 0
1 ABIN1906120 WB Rabbit AA 1-50 Log in to see Polyclonal 0
1 ABIN2746782 ELISA IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2624369 WB Rabbit AA 156-190 Log in to see Polyclonal 0
1 ABIN1087338 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1087337 ELISA WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5647872 WB Rabbit IgG AA 575-617 Log in to see Polyclonal 0
1 ABIN2934403 ELISA IF/ICC IHC WB Rabbit AA 368-625 Log in to see Polyclonal 0
1 ABIN5558750 ELISA IHC IP WB Biotin Rabbit Log in to see Polyclonal 0

General

抗原 IL2-Inducible T-Cell Kinase (ITK) 抗体
抗原表位 AA 1-26
(24), (17), (14), (10), (9), (9), (7), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
适用 人, 小鼠
(113), (26), (19), (4), (2), (1), (1), (1)
宿主 小鼠
(65), (33), (27)
克隆类型 (克隆位点)
单克隆   ( )
标记 This ITK antibody is un-conjugated
(7), (7), (6), (6), (6), (6)
应用范围 Immunoprecipitation (IP), Western Blotting (WB)
(111), (82), (9), (9), (9), (9), (8), (5), (3), (2), (1)
Pubmed 3个引用可参考
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ITK抗体详情

ITK目标详情 使用细节 贮存及处理 References for anti-ITK antibody (ABIN967287) 图像
品牌 BD Pharmingen™
产品特性 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
免疫原 Fusion protein with aa.1-26 of human/mouse Itk
克隆位点 2F12
亚型 IgG1

ITK目标详情

ITK抗体详情 使用细节 贮存及处理 References for anti-ITK antibody (ABIN967287) 图像 回页首
抗原
别名 ITK (ITK Antibody 摘要)
背景 Itk (IL-2 inducible tyrosine kinase) is a member of the TEC family of intracellular tyrosine kinases. Members of this family are characterized by their role in hematopoietic cell signaling. Itk is expressed primarily in T cells and has been shown to play a role in T-cell antigen receptor (TCR) mediated proliferation, interleukin-2 production, and T cell differentiation. Itk, following stimulation of the TCR becomes phosphorylated on tyrosine 511 by the Src kinase Lck resulting in increased in vitro Itk kinase activity. If phenylalanine is substituted for tyrosine at position 511, the in vivo kinase activity of Itk is drastically diminished, indicating that this site plays a key role in regulating Itk function. Itk migrates at ~72 kDa on SDS/PAGE. (SWISSPROT: Q08881)
分子量 72 kDa
途径 TCR Signaling, Fc-epsilon Receptor Signaling Pathway

使用细节

ITK抗体详情 ITK目标详情 贮存及处理 References for anti-ITK antibody (ABIN967287) 图像 回页首
说明

Related Products: ABIN968537

限制 仅限研究用

贮存及处理

ITK抗体详情 ITK目标详情 使用细节 References for anti-ITK antibody (ABIN967287) 图像 回页首
状态 Liquid
浓度 0.5 mg/mL
缓冲液 Aqueous buffered solution containing ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store undiluted at 4°C.

References for anti-ITK antibody (ABIN967287)

ITK抗体详情 ITK目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Liu, Bunnell, Gurniak, Berg: "T cell receptor-initiated calcium release is uncoupled from capacitative calcium entry in Itk-deficient T cells." in: The Journal of experimental medicine, Vol. 187, Issue 10, pp. 1721-7, 1998

Heyeck, Wilcox, Bunnell, Berg: "Lck phosphorylates the activation loop tyrosine of the Itk kinase domain and activates Itk kinase activity." in: The Journal of biological chemistry, Vol. 272, Issue 40, pp. 25401-8, 1997

Siliciano, Morrow, Desiderio: "itk, a T-cell-specific tyrosine kinase gene inducible by interleukin 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 23, pp. 11194-8, 1993

图像

ITK抗体详情 ITK目标详情 使用细节 贮存及处理 References for anti-ITK antibody (ABIN967287) 回页首
厂商提供的图像
Western Blotting (WB) image for anti-IL2-Inducible T-Cell Kinase (ITK) (AA 1-26) antibody (ABIN967287) Western blot analysis of Itk. Lysate from Jurkat cells was probed with anti-Itk (clon...
Western Blotting (WB) image for anti-IL2-Inducible T-Cell Kinase (ITK) (AA 1-26) antibody (ABIN967287) Immunoprecipitation/western blot analysis of Itk. Lysates from Jurkat cells were firs...
Western Blotting (WB) image for anti-IL2-Inducible T-Cell Kinase (ITK) (AA 1-26) antibody (ABIN967287) anti-IL2-Inducible T-Cell Kinase (ITK) (AA 1-26) antibody (Image 3)