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|抗原||NCK Adaptor Protein 1 (NCK1) 抗体|
|抗原表位||AA 279-377 其他选择|
|适用||小鸡, 牛, 犬, Frog, 人, 小鼠, 大鼠 其他选择|
|标记||This Cytoplasmic Protein NCK1 antibody is un-conjugated 其他选择|
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
|Supplier||Log in to see|
Cytoplasmic Protein NCK1抗体详情Cytoplasmic Protein NCK1目标详情 使用细节 贮存及处理 References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744) 图像
|交叉反应||牛, 小鸡, 犬, Frog, 小鼠, 大鼠|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|纯化方法||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|免疫原||Human NCK aa. 279-377|
Cytoplasmic Protein NCK1目标详情Cytoplasmic Protein NCK1抗体详情 使用细节 贮存及处理 References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744) 图像 回页首
|别名||NCK (NCK1 Antibody 摘要)|
|背景||NCK is a 47 kDa protein that belongs to a class of signaling molecules called adaptors. These proteins exert their effects by coupling activated receptors to downstream effectors. NCK is comprised almost entirely of one SH2 and three SH3 domains and it possesses no intrinsic catalytic activity. Investigations of its interactions with cellular proteins have found constitutive association of NCK with various Ser/THr kinases, such as p21-associated kinase (PAK) and casein kinase 1. Other proteins that directly interact with NCK are: Sos, Cbl, Wiskott-Aldrich syndrome protein (WASP), IRS1, and multiple tyrosine kinase receptors. The presence of NCK is necessary for the mitogenic response elicited by PDGF in some cells. In addition, the Drosophila homologue of NCK is involved in regulating cytoskeletal organization. This antibody is routinely tested by western blot analysis.|
|途径||TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals|
使用细节Cytoplasmic Protein NCK1抗体详情 Cytoplasmic Protein NCK1目标详情 贮存及处理 References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744) 图像 回页首
Related Products: ABIN967389
贮存及处理Cytoplasmic Protein NCK1抗体详情 Cytoplasmic Protein NCK1目标详情 使用细节 References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744) 图像 回页首
|缓冲液||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|注意事项||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|储存方法||Store undiluted at -20° C.|
References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744)Cytoplasmic Protein NCK1抗体详情 Cytoplasmic Protein NCK1目标详情 使用细节 贮存及处理 图像 回页首
Dalva, Takasu, Lin, Shamah, Hu, Gale, Greenberg: "EphB receptors interact with NMDA receptors and regulate excitatory synapse formation." in: Cell, Vol. 103, Issue 6, pp. 945-56, 2001
Miyoshi-Akiyama, Aleman, Smith, Adler, Mayer: "Regulation of Cbl phosphorylation by the Abl tyrosine kinase and the Nck SH2/SH3 adaptor." in: Oncogene, Vol. 20, Issue 30, pp. 4058-69, 2001
Schmitz, Thömmes, Beier, Wagner, Sachinidis, Düsing, Vetter: "Angiotensin II-induced stimulation of p21-activated kinase and c-Jun NH2-terminal kinase is mediated by Rac1 and Nck." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 22003-10, 2001
McManus, Boerner, Danielsen, Wang, Matsumura, Maihle: "An oncogenic epidermal growth factor receptor signals via a p21-activated kinase-caldesmon-myosin phosphotyrosine complex." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35328-34, 2000
Roche, McGlade, Jones, Gish, Pawson, Courtneidge et al.: "Requirement of phospholipase C gamma, the tyrosine phosphatase Syp and the adaptor proteins Shc and Nck for PDGF-induced DNA synthesis: evidence for the existence of Ras-dependent and Ras-independent ..." in: The EMBO journal, Vol. 15, Issue 18, pp. 4940-8, 1996
图像Cytoplasmic Protein NCK1抗体详情 Cytoplasmic Protein NCK1目标详情 使用细节 贮存及处理 References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744) 回页首