anti-大鼠 Cytoplasmic Protein NCK1 抗体 for Immunofluorescence

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

抗原
NCK Adaptor Protein 1 (NCK1) 抗体
  • NCK
  • NCKalpha
  • nck-1
  • NCK1
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • Nck-1
  • Nck1
  • nck
  • nck1-a
  • nck1-b
  • nckalpha
  • NCK adaptor protein 1
  • non-catalytic region of tyrosine kinase adaptor protein 1
  • NCK adaptor protein 1b
  • NCK adaptor protein 1 L homeolog
  • NCK1
  • Nck1
  • nck1b
  • nck1.L
抗原表位
AA 279-377
24
23
11
10
7
5
3
3
2
2
1
1
1
1
1
1
1
适用
小鸡, 牛, 犬, Frog, 人, 小鼠, 大鼠
146
30
24
19
7
4
4
3
3
2
2
2
2
2
1
1
1
1
宿主
小鼠
76
69
1
克隆类型
单克隆
标记
This Cytoplasmic Protein NCK1 antibody is un-conjugated
6
6
6
6
6
4
2
2
2
2
2
2
2
2
2
2
2
2
1
应用范围
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
137
91
65
26
14
14
7
5
4
2
2
1
1
选项
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

产品编号 ABIN967744
产品在该地区无法提供。
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.217727 ABIN967743 IF IP IHC WB Mouse IgG2b AA 279-377 Log in to see 108-NCK 5
12.217727 ABIN3197734 IC IF WB Rabbit Log in to see Polyclonal 0
1 ABIN1873839 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2888058 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5697483 ELISA FACS IF IHC IP WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1089241 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0

General

抗原 NCK Adaptor Protein 1 (NCK1) 抗体
抗原表位 AA 279-377
(24), (23), (11), (10), (7), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
适用 小鸡, 牛, 犬, Frog, 人, 小鼠, 大鼠
(146), (30), (24), (19), (7), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
宿主 小鼠
(76), (69), (1)
克隆类型 (克隆位点)
单克隆   ( )
标记 This Cytoplasmic Protein NCK1 antibody is un-conjugated
(6), (6), (6), (6), (6), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
应用范围 Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(137), (91), (65), (26), (14), (14), (7), (5), (4), (2), (2), (1), (1)
Pubmed 5个引用可参考
Supplier Log in to see

Cytoplasmic Protein NCK1抗体详情

Cytoplasmic Protein NCK1目标详情 使用细节 贮存及处理 References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744) 图像
交叉反应 牛, 小鸡, 犬, Frog, 小鼠, 大鼠
产品特性 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
免疫原 Human NCK aa. 279-377
克隆位点 108-NCK
亚型 IgG2b

Cytoplasmic Protein NCK1目标详情

Cytoplasmic Protein NCK1抗体详情 使用细节 贮存及处理 References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744) 图像 回页首
抗原
别名 NCK (NCK1 Antibody 摘要)
背景 NCK is a 47 kDa protein that belongs to a class of signaling molecules called adaptors. These proteins exert their effects by coupling activated receptors to downstream effectors. NCK is comprised almost entirely of one SH2 and three SH3 domains and it possesses no intrinsic catalytic activity. Investigations of its interactions with cellular proteins have found constitutive association of NCK with various Ser/THr kinases, such as p21-associated kinase (PAK) and casein kinase 1. Other proteins that directly interact with NCK are: Sos, Cbl, Wiskott-Aldrich syndrome protein (WASP), IRS1, and multiple tyrosine kinase receptors. The presence of NCK is necessary for the mitogenic response elicited by PDGF in some cells. In addition, the Drosophila homologue of NCK is involved in regulating cytoskeletal organization. This antibody is routinely tested by western blot analysis.
分子量 47 kDa
途径 TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

使用细节

Cytoplasmic Protein NCK1抗体详情 Cytoplasmic Protein NCK1目标详情 贮存及处理 References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744) 图像 回页首
说明

Related Products: ABIN967389

限制 仅限研究用

贮存及处理

Cytoplasmic Protein NCK1抗体详情 Cytoplasmic Protein NCK1目标详情 使用细节 References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744) 图像 回页首
状态 Liquid
浓度 250 μg/mL
缓冲液 Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store undiluted at -20° C.

References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744)

Cytoplasmic Protein NCK1抗体详情 Cytoplasmic Protein NCK1目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Dalva, Takasu, Lin, Shamah, Hu, Gale, Greenberg: "EphB receptors interact with NMDA receptors and regulate excitatory synapse formation." in: Cell, Vol. 103, Issue 6, pp. 945-56, 2001

Miyoshi-Akiyama, Aleman, Smith, Adler, Mayer: "Regulation of Cbl phosphorylation by the Abl tyrosine kinase and the Nck SH2/SH3 adaptor." in: Oncogene, Vol. 20, Issue 30, pp. 4058-69, 2001

Schmitz, Thömmes, Beier, Wagner, Sachinidis, Düsing, Vetter: "Angiotensin II-induced stimulation of p21-activated kinase and c-Jun NH2-terminal kinase is mediated by Rac1 and Nck." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 22003-10, 2001

McManus, Boerner, Danielsen, Wang, Matsumura, Maihle: "An oncogenic epidermal growth factor receptor signals via a p21-activated kinase-caldesmon-myosin phosphotyrosine complex." in: The Journal of biological chemistry, Vol. 275, Issue 45, pp. 35328-34, 2000

Roche, McGlade, Jones, Gish, Pawson, Courtneidge et al.: "Requirement of phospholipase C gamma, the tyrosine phosphatase Syp and the adaptor proteins Shc and Nck for PDGF-induced DNA synthesis: evidence for the existence of Ras-dependent and Ras-independent ..." in: The EMBO journal, Vol. 15, Issue 18, pp. 4940-8, 1996

图像

Cytoplasmic Protein NCK1抗体详情 Cytoplasmic Protein NCK1目标详情 使用细节 贮存及处理 References for anti-Cytoplasmic Protein NCK1 antibody (ABIN967744) 回页首
厂商提供的图像
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (ABIN967744) Western blot anlaysis of NCK on HeLa lysate. Lane 1: 1:1000, lane 2: 1: 2000, lane 3:...
Immunofluorescence (IF) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (ABIN967744) Immunofluorescent staining on HISM cells.
 image for anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (ABIN967744) anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (Image 3)
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (ABIN967744) anti-NCK Adaptor Protein 1 (NCK1) (AA 279-377) antibody (Image 4)