anti-人 Cytoplasmic Protein NCK1 抗体 for Western Blotting

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

抗原
NCK Adaptor Protein 1 (NCK1) 抗体
  • NCK
  • NCKalpha
  • nck-1
  • NCK1
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • Nck-1
  • Nck1
  • nck
  • nck1-a
  • nck1-b
  • nckalpha
  • NCK adaptor protein 1
  • non-catalytic region of tyrosine kinase adaptor protein 1
  • NCK adaptor protein 1b
  • NCK adaptor protein 1 L homeolog
  • NCK1
  • Nck1
  • nck1b
  • nck1.L
适用
人, 猴
131
36
27
6
3
3
3
3
2
2
2
2
2
1
1
1
宿主
小鼠
87
43
1
克隆类型
单克隆
标记
This Cytoplasmic Protein NCK1 antibody is un-conjugated
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
应用范围
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
129
76
53
14
13
11
8
6
5
2
1
1
1
选项
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产品编号 ABIN969545
$ 430.10
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19.802607 ABIN1845767 FACS IHC ELISA WB Mouse IgG1 Log in to see 0
16.802607 ABIN393431 ELISA WB Mouse IgG1 kappa AA 185-294 Log in to see 1A1 5
16.802607 ABIN4880888 ELISA FACS IHC WB Mouse IgG1 Log in to see 5B7 0
16.802607 ABIN1108388 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 5B7 2
16.55837 ABIN3021268 IHC WB Rabbit IgG Log in to see Polyclonal 0
16.55837 ABIN5663589 IF IHC WB Rabbit Log in to see Polyclonal 0
13.802607 ABIN1873840 IHC WB Rabbit IgG Log in to see Polyclonal 0
13.802607 ABIN561929 ELISA WB Mouse IgG1 kappa AA 185-294, partial Log in to see 1A1 0
12.755764 ABIN6388766 ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.755764 ABIN5621931 WB Rabbit IgG Log in to see Polyclonal 0
12.755764 ABIN2147273 IP WB Mouse IgG2a lambda Log in to see AF6D7 0
12.755764 ABIN2140080 ELISA WB Mouse IgG3 kappa AA 1-377 Log in to see AT6B3 0
12.755764 ABIN2147272 ELISA WB Mouse IgG1 kappa Log in to see 11D10 0
12.755764 ABIN2147274 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.755764 ABIN6144405 WB Rabbit Log in to see Polyclonal 0
10.802607 ABIN518212 IF WB Mouse AA 1-377, full length Log in to see Polyclonal 0
10.802607 ABIN1327536 IF WB Mouse AA 1-377, full length Log in to see Polyclonal 0
10.802607 ABIN5014021 ICC IHC WB Rabbit IgG AA 2-377 Log in to see Polyclonal 0
10.802607 ABIN2997496 IHC WB Rabbit IgG Log in to see Polyclonal 0
10.802607 ABIN2917490 IF/ICC IHC IP WB Rabbit AA 2-377 Log in to see Polyclonal 0

General

抗原 NCK Adaptor Protein 1 (NCK1) 抗体
适用 人, 猴
(131), (36), (27), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
宿主 小鼠
(87), (43), (1)
克隆类型 (克隆位点)
单克隆   ( )
标记 This Cytoplasmic Protein NCK1 antibody is un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(129), (76), (53), (14), (13), (11), (8), (6), (5), (2), (1), (1), (1)
Pubmed 2个引用可参考
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Cytoplasmic Protein NCK1抗体详情

Cytoplasmic Protein NCK1目标详情 使用细节 贮存及处理 References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545) 图像
纯化方法 Purified
免疫原 Purified recombinant fragment of human NCK1 expressed in E. Coli.
克隆位点 5B7
亚型 IgG1
Plasmids, Primers & others

Cytoplasmic Protein NCK1目标详情

Cytoplasmic Protein NCK1抗体详情 使用细节 贮存及处理 References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545) 图像 回页首
抗原
别名 NCK1 (NCK1 Antibody 摘要)
背景 The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
分子量 47 kDa
基因ID 4690
HGNC 4690
途径 TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

使用细节

Cytoplasmic Protein NCK1抗体详情 Cytoplasmic Protein NCK1目标详情 贮存及处理 References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545) 图像 回页首
应用备注 ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400
限制 仅限研究用

贮存及处理

Cytoplasmic Protein NCK1抗体详情 Cytoplasmic Protein NCK1目标详情 使用细节 References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545) 图像 回页首
状态 Liquid
缓冲液 PBS with 0.05 % sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C,-20 °C
储存方法 4°C, -20°C for long term storage

References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545)

Cytoplasmic Protein NCK1抗体详情 Cytoplasmic Protein NCK1目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Preisinger, Kolch: "The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway." in: Cellular signalling, Vol. 22, Issue 5, pp. 848-56, 2010

Blasutig, New, Thanabalasuriar, Dayarathna, Goudreault, Quaggin, Li, Gruenheid, Jones, Pawson: "Phosphorylated YDXV motifs and Nck SH2/SH3 adaptors act cooperatively to induce actin reorganization." in: Molecular and cellular biology, Vol. 28, Issue 6, pp. 2035-46, 2008

图像

Cytoplasmic Protein NCK1抗体详情 Cytoplasmic Protein NCK1目标详情 使用细节 贮存及处理 References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545) 回页首
厂商提供的图像
ELISA image for anti-NCK Adaptor Protein 1 (NCK1) antibody (ABIN969545) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-NCK Adaptor Protein 1 (NCK1) antibody (ABIN969545) Figure 2: Flow cytometric analysis of Jurkat cells using NCK1 mouse mAb (green) and n...
Immunohistochemistry (IHC) image for anti-NCK Adaptor Protein 1 (NCK1) antibody (ABIN969545) Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues usi...
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) antibody (ABIN969545) Figure 4: Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HE...
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) antibody (ABIN969545) Figure 5: Western blot analysis using NCK1 mAb against human NCK1 (AA: 203-371) recom...