anti-人 CREB1 抗体 for Immunocytochemistry

Recommended CREB1 Antibody (supplied by: Log in to see )

抗原
CAMP Responsive Element Binding Protein 1 (CREB1) 抗体
  • creb
  • CREB1
  • Creb1
  • CREB
  • 2310001E10Rik
  • 3526402H21Rik
  • AV083133
  • Creb
  • Creb-1
  • creb1
  • zgc:55598
  • cAMP responsive element binding protein 1
  • cAMP responsive element binding protein
  • cAMP responsive element binding protein 1 S homeolog
  • cAMP responsive element binding protein 1a
  • CREB1
  • creb1
  • CREB
  • Creb1
  • creb1.S
  • creb1a
适用
人, 小鼠, 大鼠, 猴
551
330
299
42
39
38
36
31
27
26
19
17
15
7
7
7
2
2
1
1
1
宿主
小鼠
450
100
12
1
克隆类型
单克隆
标记
This CREB1 antibody is un-conjugated
20
17
16
8
7
6
6
6
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
应用范围
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
445
250
193
98
90
45
42
42
32
11
11
10
4
3
3
2
2
1
1
1
选项
Supplier
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Available images

产品编号 ABIN969061
$ 430.10
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
25.611553 ABIN261497 ICC IF IHC (p) IP IHC ELISA WB Mouse IgG1 Log in to see LB9 1
1 ABIN257735 ICC IF IHC (p) IP IHC PLA WB Rabbit IgG AA 250-300 Log in to see Polyclonal 0
1 ABIN1871996 ICC IP IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4300444 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN294690 ELISA ICC IF IHC IHC (p) WB Rabbit pSer133 Log in to see Polyclonal 0
1 ABIN4300443 ELISA ICC IF IHC WB Mouse IgG1 Log in to see 5G3 0
1 ABIN2596541 ICC IF IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN2740072 ICC IF IHC IHC (p) WB Rabbit Internal Region Log in to see Polyclonal 0
1 ABIN4300441 ICC IF IHC IHC (p) Rabbit pSer129 Log in to see Polyclonal 0
1 ABIN4300445 ChIP ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN4300442 ICC IF WB Mouse IgG2 pSer133 Log in to see MM0704-13B29 0
1 ABIN1962290 ELISA FACS ICC IF WB Alkaline Phosphatase (AP) Rabbit IgG AA 105-132 Log in to see Polyclonal 0
1 ABIN1964669 ELISA FACS ICC IF WB APC Rabbit IgG AA 105-132 Log in to see Polyclonal 0
1 ABIN1973711 ELISA FACS ICC IF WB HRP Rabbit IgG AA 105-132 Log in to see Polyclonal 0
1 ABIN1968793 ELISA FACS ICC IF WB Biotin Rabbit IgG AA 105-132 Log in to see Polyclonal 0
1 ABIN1971097 ELISA FACS ICC IF WB FITC Rabbit IgG AA 105-132 Log in to see Polyclonal 0
1 ABIN1975681 ELISA FACS ICC IF WB PE Rabbit IgG AA 105-132 Log in to see Polyclonal 0
1 ABIN2740071 ChIP ICC IF IHC IHC (p) WB Rabbit IgG AA 123-136 Log in to see Polyclonal 0
1 ABIN204143 ICC ELISA Mouse IgG AA 136-341 Log in to see 0
1 ABIN5680966 ELISA ICC IF IHC WB Mouse IgG1 Log in to see 5G3 0

General

抗原 CAMP Responsive Element Binding Protein 1 (CREB1) 抗体
适用 人, 小鼠, 大鼠, 猴
(551), (330), (299), (42), (39), (38), (36), (31), (27), (26), (19), (17), (15), (7), (7), (7), (2), (2), (1), (1), (1)
宿主 小鼠
(450), (100), (12), (1)
克隆类型 (克隆位点)
单克隆   ( )
标记 This CREB1 antibody is un-conjugated
(20), (17), (16), (8), (7), (6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1)
应用范围 Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(445), (250), (193), (98), (90), (45), (42), (42), (32), (11), (11), (10), (4), (3), (3), (2), (2), (1), (1), (1)
Pubmed 3个引用可参考
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CREB1抗体详情

CREB1目标详情 使用细节 贮存及处理 References for anti-CREB1 antibody (ABIN969061) 图像
纯化方法 Ascites
免疫原 Purified recombinant fragment of human CREB1 expressed in E. Coli.
克隆位点 5G3
亚型 IgG1

CREB1目标详情

CREB1抗体详情 使用细节 贮存及处理 References for anti-CREB1 antibody (ABIN969061) 图像 回页首
抗原
别名 CREB1 (CREB1 Antibody 摘要)
背景 This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. (provided by RefSeq)
分子量 43 kDa
基因ID 1385
HGNC 1385
途径 TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation

使用细节

CREB1抗体详情 CREB1目标详情 贮存及处理 References for anti-CREB1 antibody (ABIN969061) 图像 回页首
应用备注 ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000
限制 仅限研究用

贮存及处理

CREB1抗体详情 CREB1目标详情 使用细节 References for anti-CREB1 antibody (ABIN969061) 图像 回页首
状态 Liquid
缓冲液 Ascitic fluid containing 0.03 % sodium azide.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C,-20 °C
储存方法 4°C, -20°C for long term storage

References for anti-CREB1 antibody (ABIN969061)

CREB1抗体详情 CREB1目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Jansson, Ng, Fu, Depatie, Al Azzabi, Screaton: "Glucose controls CREB activity in islet cells via regulated phosphorylation of TORC2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 29, pp. 10161-6, 2008

Mamdani, Alda, Grof, Young, Rouleau, Turecki: "Lithium response and genetic variation in the CREB family of genes." in: American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, Vol. 147B, Issue 4, pp. 500-4, 2008

Seo, Chung: "CREB activates proteasomal degradation of DSCR1/RCAN1." in: FEBS letters, Vol. 582, Issue 13, pp. 1889-93, 2008

图像

CREB1抗体详情 CREB1目标详情 使用细节 贮存及处理 References for anti-CREB1 antibody (ABIN969061) 回页首
厂商提供的图像
ELISA image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Immunocytochemistry (ICC) image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061) Figure 2: Immunofluorescence analysis of Hela cells using CREB1 mouse mAb (green) . R...
Immunohistochemistry (IHC) image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061) Figure 3: Immunohistochemical analysis of paraffin-embedded prostate cancer tissues (...
Western Blotting (WB) image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061) Figure 4: Western blot analysis using CREB1 mouse mAb against K562 (1), Jurkat (2), L...
'独立验证'标志
抗原 CREB1
Lot Number 110517
Method validated Western Blotting
Positive Control primary mouse microglia
Negative Control siRNA knock-down of CREB1 in primary mouse microglia
Notes Passed. ABIN969061 specifically recognizes Creb1 in primary mouse microglia lysates.
Primary Antibody ABIN969061
Secondary Antibody anti-mouse HRP-conjugated antibody (DakoCytomation, P0447, lot 00058737)
实验流程
  • Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
  • Transfect cells with 0.05nmol mouse Creb1 Silencer Select siRNA (ThermoFisher Scientific, ASO1093M, ASO10982 and ASO109CG, lot AMO04BXD) using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions.
  • Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, containing protease inhibitors (Sigma)).
  • Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
  • Denature 5µg of total protein for 5min at 95°C in 5µl Laemmli SDS sample buffer and subsequently separate them on a denaturing, freshly cast 10% SDS-PAGE for 1h at 140V.
  • Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
  • Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk ON at 4°C.
  • Incubation with primary mouse anti-CREB1 antibody (antibodies-online, ABIN969061, lot 110517) diluted 1:10000 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST.
  • Incubation with anti-mouse horseradish peroxidase-conjugated secondary antibody (DakoCytomation, P0447, lot 00058737) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST and 3x 5min with TBS.
  • Reveal protein bands using self-made ECL Western Blotting Detection Reagent with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
  • Strip membranes using RestoreTM Western Blot Stripping Buffer (Thermo Scientific, 21059, lot NA 164269) and subsequently incubate with a GAPDH loading control antibody (Sigma, G9545, lot 049K4787) diluted 1:10000 and with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000.
  • Wash and reveal the membrane as described above.
Experimental Notes The CREB1 antibody ABIN969061 reveals a protein of the expected molecular weight of murine Creb1 in lysates of primary mouse microglia. The protein band’s intensity decreases upon siRNA knockdown.
Validation Images
Western Blotting validation image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) antibody (ABIN969061) Primary mouse microglia were transfected with Creb1 siRNA and Creb1 was revealed usin...