anti-大鼠 TEK 抗体 for Immunohistochemistry

Recommended TEK Antibody

抗原
TEK Tyrosine Kinase, Endothelial (TEK) 抗体
  • TEK
  • DKFZp469E064
  • AA517024
  • Cd202b
  • Hyk
  • Tie2
  • tie-2
  • CD202B
  • TIE-2
  • TIE2
  • VMCM
  • VMCM1
  • Tie-2
  • tie2
  • TEK receptor tyrosine kinase
  • angiopoietin-1 receptor
  • TEK tyrosine kinase, endothelial
  • TEK
  • LOC100408334
  • tek
  • Tek
抗原表位
pTyr992
27
25
22
20
16
15
11
10
10
8
8
8
6
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
适用
人, 小鼠, 大鼠
287
162
99
9
8
宿主

235
64
10
3
2
1
克隆类型
多克隆
标记
This TEK antibody is un-conjugated
21
14
10
8
8
8
8
8
8
7
6
4
4
4
4
4
4
1
应用范围
Immunohistochemistry (IHC), Western Blotting (WB)
172
128
68
53
48
22
21
19
16
12
8
6
6
3
选项

Available images

产品编号 ABIN6256818
$ 390.77
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
10.087153 ABIN6265567 ELISA IHC WB Rabbit IgG Polyclonal 0
10.087153 ABIN6566665 IHC WB Rabbit IgG Polyclonal 0
10.087153 ABIN2432353 IHC ELISA Rabbit IgG Polyclonal 0
10.087153 ABIN2426973 IHC ELISA Rabbit IgG Polyclonal 0
7 ABIN1863207 ICC IHC WB Rabbit IgG AA 44-341 Polyclonal 0
7 ABIN2914059 IF/ICC IHC IP WB Rabbit AA 44-341 Polyclonal 0
4 ABIN6718651 IF IHC WB Rabbit Polyclonal 0
1 ABIN2751707 DB ELISA IHC IP WB FITC Chicken IgY AA 540-590, Isoform 2 Polyclonal 0
1 ABIN2751706 DB ELISA IHC IP WB FITC Rabbit IgG AA 540-590, Isoform 2 Polyclonal 0
1 ABIN2751704 DB ELISA IHC IP WB Rabbit IgG AA 540-590, Isoform 2 Polyclonal 0
1 ABIN2751705 DB ELISA IHC IP WB Chicken IgY AA 540-590, Isoform 2 Polyclonal 0
1 ABIN6337755 IF/ICC IHC IP WB Mouse 0
1 ABIN5557669 DB ELISA IHC IP WB Biotin Rabbit Polyclonal 0
1 ABIN5557670 DB ELISA IHC IP WB Biotin Chicken Polyclonal 0

General

抗原 TEK Tyrosine Kinase, Endothelial (TEK) 抗体
抗原表位 pTyr992
(27), (25), (22), (20), (16), (15), (11), (10), (10), (8), (8), (8), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用 人, 小鼠, 大鼠
(287), (162), (99), (9), (8)
宿主
(235), (64), (10), (3), (2), (1)
克隆类型
多克隆
标记 This TEK antibody is un-conjugated
(21), (14), (10), (8), (8), (8), (8), (8), (8), (7), (6), (4), (4), (4), (4), (4), (4), (1)
应用范围 Immunohistochemistry (IHC), Western Blotting (WB)
(172), (128), (68), (53), (48), (22), (21), (19), (16), (12), (8), (6), (6), (3)
Supplier

TEK抗体详情

TEK目标详情 使用细节 贮存及处理 图像
特异性 Phospho-Tie2 (Tyr992) Antibody detects endogenous levels of Tie2.
交叉反应 人, 小鼠, 大鼠
纯化方法 The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
免疫原 A synthesized peptide derived from human Tie2 around the phosphorylation site of Tyr992.
亚型 IgG
Plasmids, Primers & others

TEK目标详情

TEK抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 Tie2 (TEK Antibody 摘要)
背景

Description: Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1, SHC1 and TIE1.

Gene: TEK

分子量 160kDa
基因ID 7010
UniProt Q02763
途径 RTK signaling, Growth Factor Binding

使用细节

TEK抗体详情 TEK目标详情 贮存及处理 图像 回页首
应用备注 WB 1:500-1:2000, IHC 1:50-1:200
限制 仅限研究用

贮存及处理

TEK抗体详情 TEK目标详情 使用细节 图像 回页首
状态 Liquid
浓度 1 mg/mL
缓冲液 Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store at -20 °C.Stable for 12 months from date of receipt
有效期 12 months

图像

TEK抗体详情 TEK目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Western Blotting (WB) image for anti-TEK Tyrosine Kinase, Endothelial (TEK) (pTyr992) antibody (ABIN6256818) Western blot analysis of extracts of 293 cells transfected with wild-type Tie2, using...
Immunohistochemistry (IHC) image for anti-TEK Tyrosine Kinase, Endothelial (TEK) (pTyr992) antibody (ABIN6256818) ABIN6267197 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formal...