anti-小鼠 TEK 抗体 for Intracellular Staining

Recommended TEK Antibody

抗原
TEK Tyrosine Kinase, Endothelial (TEK) 抗体
  • TEK
  • DKFZp469E064
  • AA517024
  • Cd202b
  • Hyk
  • Tie2
  • tie-2
  • CD202B
  • TIE-2
  • TIE2
  • VMCM
  • VMCM1
  • Tie-2
  • tie2
  • TEK receptor tyrosine kinase
  • angiopoietin-1 receptor
  • TEK tyrosine kinase, endothelial
  • TEK
  • LOC100408334
  • tek
  • Tek
抗原表位
pTyr992
28
25
22
20
16
15
11
10
10
8
8
8
6
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
适用
人, 小鼠
288
163
100
9
8
宿主
小鼠
236
64
10
3
2
1
克隆类型
单克隆
标记
This TEK antibody is conjugated to Alexa Fluor 647
21
14
10
8
8
8
8
8
8
7
6
4
4
4
4
4
4
1
应用范围
Intracellular Staining (ICS)
173
128
69
53
48
22
21
19
16
12
8
6
6
3
选项
产品编号 ABIN1177217
产品在该地区无法提供。
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
10 ABIN1177218 ICS PE Mouse IgG2a, kappa pTyr1102 K93-754 6
10 ABIN1177219 ICS Alexa Fluor 647 Mouse IgG2a, kappa pTyr1102 K93-754 6
10 ABIN1177220 ICS Alexa Fluor 488 Mouse IgG2a, kappa pTyr1102 K93-754 6

General

抗原 TEK Tyrosine Kinase, Endothelial (TEK) 抗体
抗原表位 pTyr992
(28), (25), (22), (20), (16), (15), (11), (10), (10), (8), (8), (8), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用 人, 小鼠
(288), (163), (100), (9), (8)
宿主 小鼠
(236), (64), (10), (3), (2), (1)
克隆类型 (克隆位点)
单克隆   ( )
标记 This TEK antibody is conjugated to Alexa Fluor 647
(21), (14), (10), (8), (8), (8), (8), (8), (8), (7), (6), (4), (4), (4), (4), (4), (4), (1)
应用范围 Intracellular Staining (ICS)
(173), (128), (69), (53), (48), (22), (21), (19), (16), (12), (8), (6), (6), (3)
Pubmed 6个引用可参考
Supplier

TEK抗体详情

TEK目标详情 使用细节 贮存及处理 References for anti-TEK antibody (ABIN1177217) 图像
品牌 BD Phosflow™
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
免疫原 Phosphorylated Human TIE2 Peptide
克隆位点 K91-860
亚型 IgG1, kappa
Plasmids, Primers & others

TEK目标详情

TEK抗体详情 使用细节 贮存及处理 References for anti-TEK antibody (ABIN1177217) 图像 回页首
抗原
别名 TIE2 (TEK Antibody 摘要)
背景 TIE2 (Tyrosine kinase with Immunoglobulin-like and EGF-like domains 2), also known as TEK (Tunical Endothelial Kinase), is an endothelial and hematopoietic cell-specific receptor tyrosine kinase (RTK) that is critical to the development and maintenance of the vasculature and highly conserved among vertebrate species. The angiopoietins are ligands of TIE2, and the abnormal vascular growth that often occurs in solid tumors is a result of disruptions in the coordinated actions of the angiopoietins, TIE2, and the closely related TIE1 RTK. Upon activation by angiopoietins, TIE2 autophosphorylates at least 2 tyrosines in its protein kinase domain and at least 3 tyrosines in its C-terminal tail. The K91-860 monoclonal antibody recognizes the phosphorylated tyrosine 992 (Y992) in the protein kinase domain of activated TIE2. The orthologous phosphorylation site in mouse TIE2 is Y990.
Synonyms: TEK, VMCM, CD202b
途径 RTK signaling, Growth Factor Binding

使用细节

TEK抗体详情 TEK目标详情 贮存及处理 References for anti-TEK antibody (ABIN1177217) 图像 回页首
应用备注 Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation.
样本量 20 μL
限制 仅限研究用

贮存及处理

TEK抗体详情 TEK目标详情 使用细节 References for anti-TEK antibody (ABIN1177217) 图像 回页首
状态 Liquid
缓冲液 Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-TEK antibody (ABIN1177217)

TEK抗体详情 TEK目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Audero, Cascone, Maniero, Napione, Arese, Lanfrancone, Bussolino: "Adaptor ShcA protein binds tyrosine kinase Tie2 receptor and regulates migration and sprouting but not survival of endothelial cells." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 13224-33, 2004

Peters, Kontos, Lin, Wong, Rao, Huang, Dewhirst, Sankar: "Functional significance of Tie2 signaling in the adult vasculature." in: Recent progress in hormone research, Vol. 59, pp. 51-71, 2004

DeBusk, Hallahan, Lin: "Akt is a major angiogenic mediator downstream of the Ang1/Tie2 signaling pathway." in: Experimental cell research, Vol. 298, Issue 1, pp. 167-77, 2004

Jones, Iljin, Dumont, Alitalo: "Tie receptors: new modulators of angiogenic and lymphangiogenic responses." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 4, pp. 257-67, 2001

Murray, Padrique, Pinko, McTigue: "Mechanistic effects of autophosphorylation on receptor tyrosine kinase catalysis: enzymatic characterization of Tie2 and phospho-Tie2." in: Biochemistry, Vol. 40, Issue 34, pp. 10243-53, 2001

Edgell, McDonald, Graham: "Permanent cell line expressing human factor VIII-related antigen established by hybridization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 12, pp. 3734-7, 1983