anti-人 TEK 抗体 for Western Blotting

Recommended TEK Antibody

抗原
TEK Tyrosine Kinase, Endothelial (TEK) 抗体
  • TEK
  • DKFZp469E064
  • AA517024
  • Cd202b
  • Hyk
  • Tie2
  • tie-2
  • CD202B
  • TIE-2
  • TIE2
  • VMCM
  • VMCM1
  • Tie-2
  • tie2
  • TEK receptor tyrosine kinase
  • angiopoietin-1 receptor
  • TEK tyrosine kinase, endothelial
  • TEK
  • LOC100408334
  • tek
  • Tek
抗原表位
pTyr992
28
25
22
20
16
15
11
10
10
8
8
8
6
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
适用
人, 小鼠
288
163
100
9
8
宿主

236
64
10
3
2
1
克隆类型
多克隆
标记
This TEK antibody is un-conjugated
21
14
10
8
8
8
8
8
8
7
6
4
4
4
4
4
4
1
应用范围
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Western Blotting (WB)
173
128
69
53
48
22
21
19
16
12
8
6
6
3
选项

Available images

产品编号 ABIN4900637
产品在该地区无法提供。
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
18.001062 ABIN6148993 IF WB Rabbit Polyclonal 2
18.001062 ABIN6148992 IF IHC WB Rabbit Polyclonal 1
17.391693 ABIN3182332 ELISA WB Rabbit IgG pTyr992 Polyclonal 0
16.89063 ABIN1863205 ICC IHC WB Rabbit IgG AA 1-116 Polyclonal 0
16.89063 ABIN1327048 ELISA WB Mouse IgG2a kappa AA 66-185 3A5 0
16.89063 ABIN2914057 IF/ICC IHC IP WB Rabbit AA 1-116 Polyclonal 0
16.89063 ABIN3029103 IHC ELISA WB Rabbit Ig Fraction AA 758-789 Polyclonal 0
16.501062 ABIN2745572 FACS ELISA WB Mouse IgG1 Extracellular Domain tek16 0
16.501062 ABIN2745570 FACS ELISA WB Mouse IgG1 Extracellular Domain tek2 0
16.501062 ABIN2745571 FACS ELISA WB Mouse IgG1 Extracellular Domain tek9 0
13.501062 ABIN6390615 ELISA WB Rabbit IgG Polyclonal 0
13.501062 ABIN3187262 ELISA WB Rabbit IgG Tyr1507 Polyclonal 0
13.501062 ABIN3182421 ELISA IF WB Rabbit IgG pTyr1108 Polyclonal 0
13.501062 ABIN5676011 IF IHC ELISA WB Rabbit IgG Polyclonal 0
13.501062 ABIN289555 IF IHC ELISA WB Rabbit IgG1 Polyclonal 0
13.501062 ABIN5621427 ELISA IF WB Rabbit IgG pTyr1108 Polyclonal 0
13.501062 ABIN5620978 WB Rabbit C-Term Polyclonal 0
13.501062 ABIN2153196 ELISA WB Rabbit IgG pTyr992 Polyclonal 0
13.501062 ABIN2153197 ELISA WB Rabbit IgG C-Term Polyclonal 0
13.501062 ABIN2153187 ELISA WB Mouse IgG1 2 0

General

抗原 TEK Tyrosine Kinase, Endothelial (TEK) 抗体
抗原表位 pTyr992
(28), (25), (22), (20), (16), (15), (11), (10), (10), (8), (8), (8), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用 人, 小鼠
(288), (163), (100), (9), (8)
宿主
(236), (64), (10), (3), (2), (1)
克隆类型
多克隆
标记 This TEK antibody is un-conjugated
(21), (14), (10), (8), (8), (8), (8), (8), (8), (7), (6), (4), (4), (4), (4), (4), (4), (1)
应用范围 Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Western Blotting (WB)
(173), (128), (69), (53), (48), (22), (21), (19), (16), (12), (8), (6), (6), (3)
Pubmed 14个引用可参考
Supplier

TEK抗体详情

TEK目标详情 使用细节 贮存及处理 References for anti-TEK antibody (ABIN4900637) 图像
特异性 Detects human and mouse Tie-2 when phosphorylated at Y992 in Western blots.
纯化方法 Antigen Affinity-purified
过滤 0.2 μm filtered
免疫原 Phosphopeptide containing human Tie-2 Y992 site
亚型 IgG
Plasmids, Primers & others

TEK目标详情

TEK抗体详情 使用细节 贮存及处理 References for anti-TEK antibody (ABIN4900637) 图像 回页首
抗原
别名 Tie-2 (TEK Antibody 摘要)
背景 Alternative Names: angiopoietin-1 receptor, CD202B, CD202b antigen, EC 2.7.10, EC 2.7.10.1, hTIE2, p140 TEK, soluble TIE2 variant 1, soluble TIE2 variant 2, TEK, TEK tyrosine kinase, endothelial, Tie2, TIE-2, TIE2CD202b, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, venous malformations, multiple cutaneous and mucosal, VMCM, VMCM1
基因ID 7010
途径 RTK signaling, Growth Factor Binding

使用细节

TEK抗体详情 TEK目标详情 贮存及处理 References for anti-TEK antibody (ABIN4900637) 图像 回页首
应用备注 Western Blot 0.5 μg/mL
Intracellular Staining by Flow Cytometry 0.25 μg/10^6 cells
CyTOF-ready
限制 仅限研究用

贮存及处理

TEK抗体详情 TEK目标详情 使用细节 References for anti-TEK antibody (ABIN4900637) 图像 回页首
溶解方式 Reconstitute at 0.2 mg/mL in sterile PBS.
缓冲液 Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
储存液 Without preservative
储存条件 4 °C,-20 °C,-80 °C
储存方法 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
有效期 12 months

References for anti-TEK antibody (ABIN4900637)

TEK抗体详情 TEK目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Higgins, De Ceunynck, Kellum, Chen, Gu, Chaudhry, Schulman, Libermann, Lu, Shapiro, Christiani, Flaumenhaft, Parikh: "Tie2 protects the vasculature against thrombus formation in systemic inflammation." in: The Journal of clinical investigation, Vol. 128, Issue 4, pp. 1471-1484, 2018 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Ko, Kim: "Cooperation between ZEB2 and Sp1 promotes cancer cell survival and angiogenesis during metastasis through induction of survivin and VEGF." in: Oncotarget, Vol. 9, Issue 1, pp. 726-742, 2018 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Kato, Diéguez-Hurtado, Park, Hong, Kato-Azuma, Adams, Stehling, Trappmann, Wrana, Koh, Adams: "Pulmonary pericytes regulate lung morphogenesis." in: Nature communications, Vol. 9, Issue 1, pp. 2448, 2018 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Leppänen, Saharinen, Alitalo: "Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 17, pp. 4376-4381, 2017 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Shlamkovich, Aharon, Barton, Papo: "Utilizing combinatorial engineering to develop Tie2 targeting antagonistic angiopoetin-2 ligands as candidates for anti-angiogenesis therapy." in: Oncotarget, Vol. 8, Issue 20, pp. 33571-33585, 2017 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Alawo, Tahir, Fischer, Bates, Amirova, Brindle: "Regulation of Angiopoietin Signalling by Soluble Tie2 Ectodomain and Engineered Ligand Trap." in: Scientific reports, Vol. 7, Issue 1, pp. 3658, 2017 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Shah, Birdsey, Peghaire, Pitulescu, Dufton, Yang, Weinberg, Osuna Almagro, Payne, Mason, Gerhardt, Adams, Randi: "The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability." in: Nature communications, Vol. 8, pp. 16002, 2017 Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Human).

Kim, Park, Bae, Park, Kim, Lee, Song, Chung, Lim, Kubota, Hong, He, Augustin, Oliver, Koh: "Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma." in: The Journal of clinical investigation, Vol. 127, Issue 10, pp. 3877-3896, 2017 Method employed by authors: Immunohistochemistry (IHC), Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Bezabeh, Fleming, Fazenbaker, Zhong, Coffman, Yu, Leow, Gibson, Wilson, Stover, Wu, Gao, Dimasi: "Insertion of scFv into the hinge domain of full-length IgG1 monoclonal antibody results in tetravalent bispecific molecule with robust properties." in: mAbs, Vol. 9, Issue 2, pp. 240-256, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Jenny Zhou, Qin, Zhang, Tang, Ji, He, Liang, Wang, Yuan, Vortmeyer, Toomre, Fuh, Yan, Kluger, Wu, Min: "Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation." in: Nature medicine, Vol. 22, Issue 9, pp. 1033-1042, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Shah, Lueschen, Ardestani, Oberholzer, Olerud, Carlsson, Maedler: "Angiopoetin-2 Signals Do Not Mediate the Hypervascularization of Islets in Type 2 Diabetes." in: PLoS ONE, Vol. 11, Issue 9, pp. e0161834, 2016 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Woo, Qu, Babaev, Linton, Guzman, Fazio, Baldwin: "Tie1 attenuation reduces murine atherosclerosis in a dose-dependent and shear stress-specific manner." in: The Journal of clinical investigation, Vol. 121, Issue 4, pp. 1624-35, 2011 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Saharinen, Eklund, Miettinen, Wirkkala, Anisimov, Winderlich, Nottebaum, Vestweber, Deutsch, Koh, Olsen, Alitalo: "Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts." in: Nature cell biology, Vol. 10, Issue 5, pp. 527-37, 2008 Method employed by authors: Immunocytochemistry (ICC), Immunocytochemistry (ICC), Immunocytochemistry (ICC) (Sample species: Human).

Bai, Cui, Meng, Shen, He, Zhang, Chen, Xiao: "Ectopic expression of angiopoietin-1 promotes neuronal differentiation in neural progenitor cells through the Akt pathway." in: Biochemical and biophysical research communications, Vol. 378, Issue 2, pp. 296-301, 2008 Method employed by authors: Western Blotting (WB), Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

图像

TEK抗体详情 TEK目标详情 使用细节 贮存及处理 References for anti-TEK antibody (ABIN4900637) 回页首
厂商提供的图像
Flow Cytometry (FACS) image for anti-TEK Tyrosine Kinase, Endothelial (TEK) (pTyr992) antibody (ABIN4900637) Detection of Tie-2 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein...
Western Blotting (WB) image for anti-TEK Tyrosine Kinase, Endothelial (TEK) (pTyr992) antibody (ABIN4900637) Detection of Mouse Phospho-Tie-2 (Y992) by Western Blot. Western blot shows lysates o...