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|抗原||Met Proto-Oncogene (MET) 抗体|
|标记||This MET antibody is un-conjugated 其他选择|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
MET抗体详情MET目标详情 使用细节 贮存及处理 References for anti-MET antibody (ABIN358164) 图像
|特异性||This antibody detects HGF Receptor (MET) pTyr1349. Predicted to cross react with Mouse and Rabbit (100 % Antigen Homology).|
|纯化方法||Protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|免疫原||This antibody is generated from rabbits immunized with a 4=4 Chain MAPS conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1349 of human MET. Synthetic phosphopeptide was selected from region 1345~1357 of human MET.|
MET目标详情MET抗体详情 使用细节 贮存及处理 References for anti-MET antibody (ABIN358164) 图像 回页首
|别名||HGF Receptor (MET Antibody 摘要)|
|背景||The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma.Synonyms: HGF/SF receptor, Hepatocyte growth factor receptor, MET, Met proto-oncogene tyrosine kinase, Scatter factor receptor, c-Met|
|途径||RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor|
使用细节MET抗体详情 MET目标详情 贮存及处理 References for anti-MET antibody (ABIN358164) 图像 回页首
ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
贮存及处理MET抗体详情 MET目标详情 使用细节 References for anti-MET antibody (ABIN358164) 图像 回页首
|缓冲液||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|注意事项||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|注意事项||Avoid repeated freezing and thawing.|
|储存条件||4 °C/-20 °C|
|储存方法||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-MET antibody (ABIN358164)MET抗体详情 MET目标详情 使用细节 贮存及处理 图像 回页首
Wong, Roskelley, Pelech, Miller, Leung, Auersperg: "Progressive changes in Met-dependent signaling in a human ovarian surface epithelial model of malignant transformation." in: Experimental cell research, Vol. 299, Issue 1, pp. 248-56, 2004
Mineo, Costantino, Frasca, Sciacca, Russo, Vigneri, Belfiore: "Activation of the hepatocyte growth factor (HGF)-Met system in papillary thyroid cancer: biological effects of HGF in thyroid cancer cells depend on Met expression levels." in: Endocrinology, Vol. 145, Issue 9, pp. 4355-65, 2004
Chung, Yoon, Lipscomb, Mercurio: "The Met receptor and alpha 6 beta 4 integrin can function independently to promote carcinoma invasion." in: The Journal of biological chemistry, Vol. 279, Issue 31, pp. 32287-93, 2004
Fischer, Giordano, Comoglio, Ullrich: "Reactive oxygen species mediate Met receptor transactivation by G protein-coupled receptors and the epidermal growth factor receptor in human carcinoma cells." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28970-8, 2004
Higuchi, Orita, Katsuya, Yamasaki, Akiyama, Li, Yamamoto, Saito, Nakamura: "MUC20 suppresses the hepatocyte growth factor-induced Grb2-Ras pathway by binding to a multifunctional docking site of met." in: Molecular and cellular biology, Vol. 24, Issue 17, pp. 7456-68, 2004
图像MET抗体详情 MET目标详情 使用细节 贮存及处理 References for anti-MET antibody (ABIN358164) 回页首