anti-人 MET 抗体 for Enzyme Immunoassay

Recommended MET Antibody (supplied by: Log in to see )

抗原
Met Proto-Oncogene (MET) 抗体
  • AUTS9
  • HGFR
  • RCCP2
  • c-Met
  • AI838057
  • HGF
  • Par4
  • Hgfr
  • c-met
  • MET proto-oncogene, receptor tyrosine kinase
  • met proto-oncogene
  • MET
  • Met
抗原表位
pTyr1349
82
80
55
48
41
30
24
19
17
15
15
15
15
14
6
6
6
6
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
适用

710
292
222
20
15
9
6
6
5
3
3
3
3
2
2
1
1
1
1
宿主

443
272
5
5
4
克隆类型
多克隆
标记
This MET antibody is un-conjugated
25
24
24
19
19
13
12
10
8
7
7
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
4
1
1
应用范围
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
512
211
141
130
100
85
65
39
24
19
12
10
7
6
3
2
1
1
1
1
1
1
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产品编号 ABIN358164
$ 390.50
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN358165 EIA WB Rabbit Ig Fraction pTyr1356 Log in to see Polyclonal 5
1 ABIN356338 EIA WB Mouse IgG1 pTyr1234, pTyr1235 Log in to see 6AT1877 0
1 ABIN5551808 EIA WB Rabbit IgG pTyr1349, pTyr1356 Log in to see Polyclonal 0
1 ABIN5611613 EIA IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
1 ABIN317487 EIA WB Mouse IgG1 Catalytic Domain Log in to see 4AT44 0
1 ABIN5550863 EIA IHC (p) WB Mouse IgG1 Catalytic Domain Log in to see 4AT44 0
1 ABIN5550865 EIA WB Mouse IgG1 Catalytic Domain Log in to see 6AT1785 0
1 ABIN5550866 EIA WB Mouse IgG1 Catalytic Domain Log in to see 4AT259 0
1 ABIN5550867 EIA WB Mouse IgG1 Catalytic Domain Log in to see 4AT247 0
1 ABIN5550864 EIA IHC (p) WB Mouse IgG1 Catalytic Domain Log in to see 6AT203 0
1 ABIN1107526 EIA IHC (fro) WB Rabbit C-Term Log in to see Polyclonal 0
1 ABIN5542211 EIA FACS Rat IgG2b Log in to see BCI-3E7 0

General

抗原 Met Proto-Oncogene (MET) 抗体
抗原表位 pTyr1349
(82), (80), (55), (48), (41), (30), (24), (19), (17), (15), (15), (15), (15), (14), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用
(710), (292), (222), (20), (15), (9), (6), (6), (5), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
宿主
(443), (272), (5), (5), (4)
克隆类型
多克隆
标记 This MET antibody is un-conjugated
(25), (24), (24), (19), (19), (13), (12), (10), (8), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (1), (1)
应用范围 Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(512), (211), (141), (130), (100), (85), (65), (39), (24), (19), (12), (10), (7), (6), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 5个引用可参考
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MET抗体详情

MET目标详情 使用细节 贮存及处理 References for anti-MET antibody (ABIN358164) 图像
特异性 This antibody detects HGF Receptor (MET) pTyr1349. Predicted to cross react with Mouse and Rabbit (100 % Antigen Homology).
纯化方法 Protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
免疫原 This antibody is generated from rabbits immunized with a 4=4 Chain MAPS conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1349 of human MET. Synthetic phosphopeptide was selected from region 1345~1357 of human MET.
亚型 Ig Fraction

MET目标详情

MET抗体详情 使用细节 贮存及处理 References for anti-MET antibody (ABIN358164) 图像 回页首
抗原
别名 HGF Receptor (MET Antibody 摘要)
背景 The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma.Synonyms: HGF/SF receptor, Hepatocyte growth factor receptor, MET, Met proto-oncogene tyrosine kinase, Scatter factor receptor, c-Met
分子量 155527 Da
基因ID 4233, 9606
UniProt P08581
途径 RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor

使用细节

MET抗体详情 MET目标详情 贮存及处理 References for anti-MET antibody (ABIN358164) 图像 回页首
应用备注 ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用

贮存及处理

MET抗体详情 MET目标详情 使用细节 References for anti-MET antibody (ABIN358164) 图像 回页首
状态 Liquid
浓度 0.25 mg/mL
缓冲液 PBS with 0.09 % (W/V) Sodium Azide as preservative.
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-MET antibody (ABIN358164)

MET抗体详情 MET目标详情 使用细节 贮存及处理 图像 回页首
背景

Wong, Roskelley, Pelech, Miller, Leung, Auersperg: "Progressive changes in Met-dependent signaling in a human ovarian surface epithelial model of malignant transformation." in: Experimental cell research, Vol. 299, Issue 1, pp. 248-56, 2004

Mineo, Costantino, Frasca, Sciacca, Russo, Vigneri, Belfiore: "Activation of the hepatocyte growth factor (HGF)-Met system in papillary thyroid cancer: biological effects of HGF in thyroid cancer cells depend on Met expression levels." in: Endocrinology, Vol. 145, Issue 9, pp. 4355-65, 2004

Chung, Yoon, Lipscomb, Mercurio: "The Met receptor and alpha 6 beta 4 integrin can function independently to promote carcinoma invasion." in: The Journal of biological chemistry, Vol. 279, Issue 31, pp. 32287-93, 2004

Fischer, Giordano, Comoglio, Ullrich: "Reactive oxygen species mediate Met receptor transactivation by G protein-coupled receptors and the epidermal growth factor receptor in human carcinoma cells." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28970-8, 2004

Higuchi, Orita, Katsuya, Yamasaki, Akiyama, Li, Yamamoto, Saito, Nakamura: "MUC20 suppresses the hepatocyte growth factor-induced Grb2-Ras pathway by binding to a multifunctional docking site of met." in: Molecular and cellular biology, Vol. 24, Issue 17, pp. 7456-68, 2004

图像

MET抗体详情 MET目标详情 使用细节 贮存及处理 References for anti-MET antibody (ABIN358164) 回页首
厂商提供的图像
 image for anti-Met Proto-Oncogene (MET) (pTyr1349) antibody (ABIN358164) anti-Met Proto-Oncogene (MET) (pTyr1349) antibody
 image for anti-Met Proto-Oncogene (MET) (pTyr1349) antibody (ABIN358164) anti-Met Proto-Oncogene (MET) (pTyr1349) antibody (Image 2)