anti-人 GDNF 抗体 for Immunofluorescence (Paraffin-embedded Sections)

Recommended GDNF Antibody (supplied by: Log in to see )

抗原
Glial Cell Line Derived Neurotrophic Factor (GDNF) 抗体
  • gdnf
  • GDNF
  • AI385739
  • gdnfa
  • hfb1-gdnf
  • ATF1
  • ATF2
  • HFB1-GDNF
  • HSCR3
  • glial cell derived neurotrophic factor
  • glial cell derived neurotrophic factor a
  • glial cell line derived neurotrophic factor
  • glial cell derived neurotrophic factor L homeolog
  • Gdnf
  • gdnfa
  • GDNF
  • gdnf.L
抗原表位
AA 166-211
16
14
10
10
6
6
4
3
2
1
1
1
1
1
1
1
1
适用
人, 小鼠, 大鼠
168
77
57
1
宿主

152
22
10
4
2
克隆类型
多克隆
标记
This GDNF antibody is un-conjugated
19
11
7
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
175
57
47
28
27
21
13
12
12
6
6
5
4
2
2
1
1
选项
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产品编号 ABIN736536
$ 329.45
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
16.446873 ABIN736543 IF (p) FITC Rabbit IgG AA 166-211 Log in to see Polyclonal 0
1 ABIN898609 IF (p) Alexa Fluor 488 Rabbit IgG AA 166-211 Log in to see Polyclonal 0
1 ABIN898607 IF (p) Alexa Fluor 647 Rabbit IgG AA 166-211 Log in to see Polyclonal 0
1 ABIN898610 IF (p) Alexa Fluor 555 Rabbit IgG AA 166-211 Log in to see Polyclonal 0
1 ABIN898608 IF (p) Alexa Fluor 350 Rabbit IgG AA 166-211 Log in to see Polyclonal 0
1 ABIN736539 IF (p) Cy3 Rabbit IgG AA 166-211 Log in to see Polyclonal 0
1 ABIN736540 IF (p) Cy5 Rabbit IgG AA 166-211 Log in to see Polyclonal 0
1 ABIN736542 IF (p) Cy7 Rabbit IgG AA 166-211 Log in to see Polyclonal 0
1 ABIN736541 IF (p) Cy5.5 Rabbit IgG AA 166-211 Log in to see Polyclonal 0
1 ABIN2813671 IF (p) Alexa Fluor 594 Rabbit IgG AA 166-211 Log in to see Polyclonal 0
1 ABIN5003350 IF (p) Alexa Fluor 750 Rabbit IgG AA 166-211 Log in to see Polyclonal 0
1 ABIN5003349 IF (p) Alexa Fluor 680 Rabbit IgG AA 166-211 Log in to see Polyclonal 0

General

抗原 Glial Cell Line Derived Neurotrophic Factor (GDNF) 抗体
抗原表位 AA 166-211
(16), (14), (10), (10), (6), (6), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
适用 人, 小鼠, 大鼠
(168), (77), (57), (1)
宿主
(152), (22), (10), (4), (2)
克隆类型
多克隆
标记 This GDNF antibody is un-conjugated
(19), (11), (7), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(175), (57), (47), (28), (27), (21), (13), (12), (12), (6), (6), (5), (4), (2), (2), (1), (1)
Pubmed 找到1个引用
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GDNF抗体详情

GDNF目标详情 使用细节 贮存及处理 References for anti-GDNF antibody (ABIN736536) 图像
纯化方法 Purified by Protein A.
免疫原 KLH conjugated synthetic peptide derived from human GDNF
亚型 IgG
Plasmids, Primers & others

GDNF目标详情

GDNF抗体详情 使用细节 贮存及处理 References for anti-GDNF antibody (ABIN736536) 图像 回页首
抗原
别名 GDNF (GDNF Antibody 摘要)
背景

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Subcellular location: Secreted

Synonyms: ATF1, ATF2, HSCR3, HFB1-GDNF, Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF

基因ID 2668
UniProt P39905
途径 RTK signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration

使用细节

GDNF抗体详情 GDNF目标详情 贮存及处理 References for anti-GDNF antibody (ABIN736536) 图像 回页首
应用备注 WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
限制 仅限研究用

贮存及处理

GDNF抗体详情 GDNF目标详情 使用细节 References for anti-GDNF antibody (ABIN736536) 图像 回页首
状态 Liquid
浓度 1 μg/μL
缓冲液 Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
储存条件 -20 °C
储存方法 Store at -20°C
有效期 12 months

References for anti-GDNF antibody (ABIN736536)

GDNF抗体详情 GDNF目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Zhang, Cai, Song, Dong, Hou, Lv: "Normalization of ventral tegmental area structure following acupuncture in a rat model of heroin relapse." in: Neural regeneration research, Vol. 9, Issue 3, pp. 301-7, 2014 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Rat (Rattus)). Further details: Sample: Brain

图像

GDNF抗体详情 GDNF目标详情 使用细节 贮存及处理 References for anti-GDNF antibody (ABIN736536) 回页首
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Anti...
Immunohistochemistry (IHC) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Formalin-fixed and paraffin embedded human glioma tissue labeled with Anti-GDNF Polyc...
SDS-PAGE (SDS) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti GDNF Polyclonal ...
Western Blotting (WB) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Lane 1:U251 lysates and Lane 2: Mouse brain lysates probed with Rabbit Anti-GDNF Poly...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) Formalin-fixed and paraffin embedded rat brain labeled with Anti-GDNF Polyclonal Anti...
'独立验证'标志
抗原 GDNF
Lot Number 9F01M8
Method validated Immunofluorescence
Positive Control mouse hippocampus and putamen
Negative Control no primary control
Notes

ABIN736536 works in IF albeit with some background staining that we have been unable to eliminate although using different dilutions to increase the signal-to-noise ratio.

Primary Antibody ABIN736536
Secondary Antibody anti-rabbit AF488 conjugated antibody (Life Technologies)
实验流程
  • Perfuse mouse with 4% paraformaldehyde in 0.1M phosphate buffer (PB) pH7.4.
  • Post-fix spinal cord and brain blocks in the same fixative for additional 2h at RT.
  • Wash spinal cord and brain blocks several times with PBS.
  • Cut blocks with a vibratome (Leica, VT1000 S) into 70µm thick transverse sections.
  • Cut dorsal root ganglions (DRGs) with a cryostat into 17µm thick sections after cryoprotection and glass mounting.
  • Block free floating vibratome and glass mounted cryostat sections with blocking solution (0.01M PBS5% Normal Goat Serum (NGS; Sigma, G9023, lot SLBV1396), 0.1% Triton X-100 (BioRad, 161-0407, lot 00583) for 1h at RT.
  • Incubate sections with primary rabbit anti-GDNF antibody (antibodies-online, ABIN736536, lot 9F01M8) diluted 1:100 in blocking solution ON at RT.
  • Wash sections 4x for 5min with 0.01M PBS.
  • Incubate sections with secondary goat anti-rabbit AF488 conjugated antibody (Life Technologies) diluted 1:500 in PBS for 1h at RT.
  • Wash sections 4x for 5min with 0.01M PBS.
  • Mount sections in Fluoroshield (Sigma, F6182, lot MKCB0153V).
Experimental Notes
  • Different dilutions were also tested (1:200, 1:500, 1:2000) with or without Triton-X but the 1:100 dilution and the use of Triton-X in the blocking solution gave the best results.

  • Staining is mainly present in the cytoplasm of small-size neurons in the DRG, consistent with what has been previously described. ABIN736536 stains fibers in superficial laminae of the dorsal horn of the spinal cord. In the hippocampus staining is present in the CA1 both in the pyramidal neurons (cell body and dendrites) and in individual interneurons. In the putamen ABIN736536 stains neuron cell bodies and some dendrites.

Validation Images
Immunofluorescence validation image for anti-Glial Cell Line Derived Neurotrophic Factor (GDNF) (AA 166-211) antibody (ABIN736536) IF staining with ABIN736536 of mouse dorsal root ganglion (DRG, A), the dorsal horn o...