anti-人 PRAME 抗体 for Immunocytochemistry

Recommended PRAME Antibody (supplied by: Log in to see )

抗原
Preferentially Expressed Antigen in Melanoma (PRAME) 抗体
  • PRAME
  • CT130
  • MAPE
  • OIP-4
  • OIP4
  • 4930534P07Rik
  • preferentially expressed antigen in melanoma
  • PRAME
  • Prame
抗原表位
N-Term, AA 1-100
15
13
10
7
5
4
3
2
2
2
2
1
1
适用
人, 小鼠
74
5
4
4
4
3
2
2
1
宿主

71
4
克隆类型
多克隆
标记
This PRAME antibody is un-conjugated
5
5
3
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
53
13
12
11
10
6
5
3
2
2
2
2
1
Supplier
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Available images

产品编号 ABIN315885
产品在该地区无法提供。
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1860297 ICC IHC WB Rabbit IgG AA 416-502 Log in to see Polyclonal 0
1 ABIN1860296 ICC IHC WB Rabbit IgG AA 416-502 Log in to see Polyclonal 0

General

抗原 Preferentially Expressed Antigen in Melanoma (PRAME) 抗体
抗原表位 N-Term, AA 1-100
(15), (13), (10), (7), (5), (4), (3), (2), (2), (2), (2), (1), (1)
适用 人, 小鼠
(74), (5), (4), (4), (4), (3), (2), (2), (1)
宿主
(71), (4)
克隆类型
多克隆
标记 This PRAME antibody is un-conjugated
(5), (5), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(53), (13), (12), (11), (10), (6), (5), (3), (2), (2), (2), (2), (1)
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PRAME抗体详情

PRAME目标详情 使用细节 贮存及处理 图像
纯化方法 Immunogen affinity purified
免疫原 Synthetic peptide made to an N-terminal portion of human PRAME (within residues 1-100). [Swiss-Prot# P78395]
Plasmids, Primers & others

PRAME目标详情

PRAME抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 PRAME (PRAME Antibody 摘要)
背景 Gene Symbol: PRAME
分子量 Theoretical MW: 57 kDa
基因ID 23532
UniProt P78395
途径 Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding

使用细节

PRAME抗体详情 PRAME目标详情 贮存及处理 图像 回页首
应用备注 Western Blot 2 μg/mL, Immunocytochemistry/Immunofluorescence 1:100This PRAME antibody is useful for Western blot, where a band is seen at ~57 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
说明

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

实验流程 Protocol specific for PRAME Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 30 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-PRAME primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
限制 仅限研究用

贮存及处理

PRAME抗体详情 PRAME目标详情 使用细节 图像 回页首
状态 Liquid
浓度 1 mg/mL
缓冲液 Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.1 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Do not freeze.
储存条件 4 °C
储存方法 Store at 4°C. Do not freeze.

图像

PRAME抗体详情 PRAME目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Immunofluorescence (IF) image for anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-100), (N-Term) antibody (ABIN315885) Immunocytochemistry/Immunofluorescence: PRAME Antibody [NBP1-03359] - PRAME antibody ...
Western Blotting (WB) image for anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-100), (N-Term) antibody (ABIN315885) Western Blot: PRAME Antibody [NBP1-03359] - Detection of PRAME in MCF7 cell lysates u...