anti-人 PRAME 抗体 for Immunocytochemistry

Recommended PRAME Antibody (supplied by: Log in to see )

抗原
Preferentially Expressed Antigen in Melanoma (PRAME) 抗体
  • PRAME
  • CT130
  • MAPE
  • OIP-4
  • OIP4
  • 4930534P07Rik
  • preferentially expressed antigen in melanoma
  • PRAME
  • Prame
抗原表位
AA 416-502
15
13
9
7
5
4
2
2
2
2
1
1
适用

74
5
4
4
4
3
2
2
1
宿主

71
4
克隆类型
多克隆
标记
This PRAME antibody is un-conjugated
5
5
3
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
53
13
12
10
10
7
5
3
2
2
2
1
1
选项
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产品编号 ABIN1860297
$ 350.00
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10 ABIN1860296 ICC IHC WB Rabbit IgG AA 416-502 Log in to see Polyclonal 0

General

抗原 Preferentially Expressed Antigen in Melanoma (PRAME) 抗体
抗原表位 AA 416-502
(15), (13), (9), (7), (5), (4), (2), (2), (2), (2), (1), (1)
适用
(74), (5), (4), (4), (4), (3), (2), (2), (1)
宿主
(71), (4)
克隆类型
多克隆
标记 This PRAME antibody is un-conjugated
(5), (5), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
(53), (13), (12), (10), (10), (7), (5), (3), (2), (2), (2), (1), (1)
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PRAME抗体详情

PRAME目标详情 使用细节 贮存及处理 图像
特异性 The antibody is a rabbit polyclonal antibody raised against PRAME. It has been selected for its ability to recognize PRAME in immunohistochemical staining and western blotting.
纯化方法 Antigen-specific affinity chromatography
免疫原 PRAME (Asn416-Leu502)
亚型 IgG
Plasmids, Primers & others

PRAME目标详情

PRAME抗体详情 使用细节 贮存及处理 图像 回页首
抗原
背景 Alternative Names: MAPE, OIP4, CT130, Cancer/Testis Antigen 130, Opa-interacting protein 4, Melanoma antigen preferentially expressed in tumors
途径 Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding

使用细节

PRAME抗体详情 PRAME目标详情 贮存及处理 图像 回页首
应用备注
  • Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.
说明

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

限制 仅限研究用

贮存及处理

PRAME抗体详情 PRAME目标详情 使用细节 图像 回页首
状态 Liquid
浓度 200 μg/mL
缓冲液 PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 4 °C
储存方法 Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
有效期 12 months

图像

PRAME抗体详情 PRAME目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Western Blotting (WB) image for anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 416-502) antibody (ABIN1860297) anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 416-502) antibody
Western Blotting (WB) image for anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 416-502) antibody (ABIN1860297) Western Blot; Sample: Human A549 cell lysate; Primary Ab: 2µg/ml Rabbit Anti-Human PR...
 image for anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 416-502) antibody (ABIN1860297) Figure.DAB staining on IHC-P. Samples: Human Tissue