anti-人 PRAME 抗体 for Enzyme Immunoassay

Recommended PRAME Antibody (supplied by: Log in to see )

抗原
Preferentially Expressed Antigen in Melanoma (PRAME) 抗体
  • PRAME
  • CT130
  • MAPE
  • OIP-4
  • OIP4
  • 4930534P07Rik
  • preferentially expressed antigen in melanoma
  • PRAME
  • Prame
抗原表位
C-Term
15
10
9
8
6
3
3
3
2
2
2
2
1
1
适用

75
6
4
4
4
3
2
2
1
宿主

72
4
克隆类型
多克隆
标记
This PRAME antibody is un-conjugated
6
5
3
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Enzyme Immunoassay (EIA), Western Blotting (WB)
53
13
10
10
9
8
5
5
3
2
2
2
Supplier
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产品编号 ABIN453177
$ 390.50
加上运输费 $45.00

General

抗原 Preferentially Expressed Antigen in Melanoma (PRAME) 抗体
抗原表位 C-Term
(15), (10), (9), (8), (6), (3), (3), (3), (2), (2), (2), (2), (1), (1)
适用
(75), (6), (4), (4), (4), (3), (2), (2), (1)
宿主
(72), (4)
克隆类型
多克隆
标记 This PRAME antibody is un-conjugated
(6), (5), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Enzyme Immunoassay (EIA), Western Blotting (WB)
(53), (13), (10), (10), (9), (8), (5), (5), (3), (2), (2), (2)
Pubmed 3个引用可参考
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PRAME抗体详情

PRAME目标详情 使用细节 贮存及处理 References for anti-PRAME antibody (ABIN453177) 图像
特异性 This antibody reacts to MAPE.
纯化方法 Saturated Ammonium Sulfate (SAS) precipitation
免疫原 KLH conjugated synthetic peptide selected from the C-terminal region of human MAPE
Plasmids, Primers & others

PRAME目标详情

PRAME抗体详情 使用细节 贮存及处理 References for anti-PRAME antibody (ABIN453177) 图像 回页首
抗原
别名 PRAME / MAPE (PRAME Antibody 摘要)
背景 MAPE is an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, its gene is also expressed in acute leukemias.Synonyms: Melanoma antigen preferentially expressed in tumors, OIP-4, OIP4, OPA-interacting protein 4, Preferentially expressed antigen of melanoma
基因ID 23532
NCBI登录号 NP_006106
UniProt P78395
途径 Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding

使用细节

PRAME抗体详情 PRAME目标详情 贮存及处理 References for anti-PRAME antibody (ABIN453177) 图像 回页首
应用备注 ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
限制 仅限研究用

贮存及处理

PRAME抗体详情 PRAME目标详情 使用细节 References for anti-PRAME antibody (ABIN453177) 图像 回页首
状态 Liquid
浓度 0.25 mg/mL
缓冲液 PBS with 0.09 % (W/V) sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-PRAME antibody (ABIN453177)

PRAME抗体详情 PRAME目标详情 使用细节 贮存及处理 图像 回页首
背景

Rezvani, Yong, Tawab, Jafarpour, Eniafe, Mielke, Savani, Keyvanfar, Li, Kurlander, Barrett: "Ex vivo characterization of polyclonal memory CD8+ T-cell responses to PRAME-specific peptides in patients with acute lymphoblastic leukemia and acute and chronic myeloid leukemia." in: Blood, Vol. 113, Issue 10, pp. 2245-55, 2009

Qin, Zhu, Jiang, Li, Lu, Li, Ruan, Liu, Chen, Huang: "Expression patterns of WT1 and PRAME in acute myeloid leukemia patients and their usefulness for monitoring minimal residual disease." in: Leukemia research, Vol. 33, Issue 3, pp. 384-90, 2009

Neumann, Engelsberg, Decker, Störkel, Jaeger, Huber, Seliger: "Heterogeneous expression of the tumor-associated antigens RAGE-1, PRAME, and glycoprotein 75 in human renal cell carcinoma: candidates for T-cell-based immunotherapies?" in: Cancer research, Vol. 58, Issue 18, pp. 4090-5, 1998

图像

PRAME抗体详情 PRAME目标详情 使用细节 贮存及处理 References for anti-PRAME antibody (ABIN453177) 回页首
厂商提供的图像
Western Blotting (WB) image for anti-Preferentially Expressed Antigen in Melanoma (PRAME) (C-Term) antibody (ABIN453177) Western blot analysis of MAPE antibody (C-term) in K562 cell line lysates (35ug/lane)...