anti-猴 Arginine Vasopressin Receptor 1B 抗体 for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Arginine Vasopressin Receptor 1B Antibody (supplied by: Log in to see )

抗原
Arginine Vasopressin Receptor 1B (AVPR1B) 抗体
  • LOC443050
  • AVPR1B
  • si:ch211-125a15.3
  • AVPR3
  • V3/V1b
  • VIBR
  • VPR3
  • arginine vasopressin receptor 1B
  • arginine vasopressin receptor 1Ab
  • AVPR1B
  • avpr1ab
  • Avpr1b
抗原表位
Cytoplasmic Domain
47
17
10
10
10
7
5
4
4
2
2
2
1
1
1
1
1
1
1
适用
人, 猴
100
56
51
6
4
4
3
2
2
1
1
1
宿主

114
40
克隆类型
多克隆
标记
非结合性
10
10
10
7
6
6
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
114
86
36
30
13
7
4
2
2
1
1
1
1
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产品编号 ABIN1048334
$ 484.00
加上运输费 $45.00

General

抗原 Arginine Vasopressin Receptor 1B (AVPR1B) 抗体
抗原表位 Cytoplasmic Domain
(47), (17), (10), (10), (10), (7), (5), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
适用 人, 猴
(100), (56), (51), (6), (4), (4), (3), (2), (2), (1), (1), (1)
宿主
(114), (40)
克隆类型
多克隆
标记 非结合性
(10), (10), (10), (7), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(114), (86), (36), (30), (13), (7), (4), (2), (2), (1), (1), (1), (1)
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产品细节

目标详细情况 使用细节 贮存及处理 图像
品牌 IHC-plus™
特异性 Human AVPR1B. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
预测反应 Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Marmoset, Elephant, Bat, Pig (88%).
纯化方法 Immunoaffinity purified
免疫原 Synthetic 16 amino acid peptide from 3rd cytoplasmic domain of human AVPR1B. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Marmoset, Elephant, Bat, Pig (88%).

Type of Immunogen: Synthetic peptide

目标详细情况

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抗原
别名 AVPR1B (AVPR1B Antibody 摘要)
背景 Name/Gene ID: AVPR1B
Subfamily: Vasopressin/oxytocin
Family: GPCR

Synonyms: AVPR1B, AVPR3, V1bR, AVPR V1b, AVPR V3, V1b receptor, V1b vasopressin receptor, Vasopressin V1b receptor, Vasopressin V3 receptor, VPR3
基因ID 553
途径 Regulation of Systemic Arterial Blood Pressure by Hormones

使用细节

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应用备注 Approved: IHC, IHC-P (2 - 10 μg/mL)
说明

Target Species of Antibody: Human

实验流程 The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
限制 仅限研究用

贮存及处理

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状态 Liquid
浓度 Lot specific
缓冲液 PBS, less than 0.1 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C,-20 °C
储存方法 Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
有效期 12 months

图像

产品细节 目标详细情况 使用细节 贮存及处理 回页首
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Arginine Vasopressin Receptor 1B (AVPR1B) (Cytoplasmic Domain) antibody (ABIN1048334) Anti-AVPR1B antibody ABIN1048334 IHC staining of human pituitary, anterior. Immunohi...
Immunohistochemistry (IHC) image for anti-Arginine Vasopressin Receptor 1B (AVPR1B) (Cytoplasmic Domain) antibody (ABIN1048334) Anti-AVPR1B antibody IHC of human pituitary, anterior. Immunohistochemistry of formal...