anti-兔 Insulin 抗体 for Immunohistochemistry

Recommended Insulin Antibody (supplied by: Log in to see )

抗原
Insulin (INS) 抗体
  • ins1
  • xins
  • ins1-a
  • Insulin
  • IDDM2
  • ILPR
  • IRDN
  • MODY10
  • AA986540
  • Ins-2
  • InsII
  • Mody
  • Mody4
  • proinsulin
  • zgc:109842
  • insulin
  • insulin precursor
  • insulin II
  • preproinsulin
  • ins
  • PIN
  • INS
  • Ins
  • INS-IGF2
  • Ins2
抗原表位
AA 1-84
27
19
15
12
10
7
6
6
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
适用
牛, 人, 猪, 兔, 大鼠
876
391
263
257
206
119
49
6
6
6
5
4
4
2
2
1
1
1
1
1
1
1
宿主
小鼠
708
179
53
16
3
1
克隆类型
单克隆
标记
This Insulin antibody is un-conjugated
47
38
37
23
22
22
22
17
17
14
14
14
14
14
14
14
14
10
9
8
8
8
5
5
5
5
5
5
2
1
应用范围
Immunofluorescence (IF), Immunohistochemistry (IHC)
507
353
229
204
190
181
146
124
66
64
33
26
15
11
8
6
5
4
4
3
3
2
2
1
1
1
1
1
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产品编号 ABIN3026326
$ 361.90
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.067091 ABIN3026546 IF IHC Mouse IgG1, kappa AA 1-84 Log in to see E2-E3 or INS04 0
9.067091 ABIN3025694 IF IHC Mouse IgG1, kappa AA 1-84 Log in to see SPM531 0
1 ABIN269653 DB ICC IF IHC (p) IHC RIA Mouse IgG1 Log in to see K36aC10 0
1 ABIN3026547 IHC Mouse IgG1, kappa AA 1-84 Log in to see E2-E3 or INS04 0
1 ABIN3026327 IHC Mouse IgG1, kappa AA 1-84 Log in to see E2-E3-2D11-H5 or INS04-INS05 0
1 ABIN3026328 IF IHC Mouse IgG1, kappa AA 1-84 Log in to see E2-E3-2D11-H5 or INS04-INS05 0
1 ABIN3026548 IF IHC Mouse IgG1, kappa AA 1-84 Log in to see E2-E3 or INS04 0
1 ABIN3025690 IHC Mouse IgG1, kappa Log in to see IRDN-805 0
1 ABIN3025695 IF IHC Mouse IgG1, kappa AA 1-84 Log in to see SPM531 0
1 ABIN2934881 ELISA IF/ICC IHC WB Guinea Pig AA 25-110 Log in to see Polyclonal 0

General

抗原 Insulin (INS) 抗体
抗原表位 AA 1-84
(27), (19), (15), (12), (10), (7), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用 牛, 人, 猪, 兔, 大鼠
(876), (391), (263), (257), (206), (119), (49), (6), (6), (6), (5), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1)
宿主 小鼠
(708), (179), (53), (16), (3), (1)
克隆类型 (克隆位点)
单克隆   ( )
标记 This Insulin antibody is un-conjugated
(47), (38), (37), (23), (22), (22), (22), (17), (17), (14), (14), (14), (14), (14), (14), (14), (14), (10), (9), (8), (8), (8), (5), (5), (5), (5), (5), (5), (2), (1)
应用范围 Immunofluorescence (IF), Immunohistochemistry (IHC)
(507), (353), (229), (204), (190), (181), (146), (124), (66), (64), (33), (26), (15), (11), (8), (6), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
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Insulin抗体详情

Insulin目标详情 使用细节 贮存及处理 图像
产品特性 This antibody cocktail recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. It enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Specific antibodies can be used as beta-cell and insulinoma markers.
纯化方法 Protein G affinity chromatography
免疫原 Full length purified pig Insulin (aa 1-84), clones E2-E3 and 2D11-H5, was used as the immunogen for this antibody cocktail.
克隆位点 E2-E3-2D11-H5 or INS04-INS05
亚型 IgG1, kappa

Insulin目标详情

Insulin抗体详情 使用细节 贮存及处理 图像 回页首
抗原
别名 Insulin (INS Antibody 摘要)
背景 This antibody cocktail recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. It enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Specific antibodies can be used as beta-cell and insulinoma markers.
基因ID 3630
途径 NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation

使用细节

Insulin抗体详情 Insulin目标详情 贮存及处理 图像 回页首
应用备注 The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for staining of formalin/paraffin tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

限制 仅限研究用

贮存及处理

Insulin抗体详情 Insulin目标详情 使用细节 图像 回页首
浓度 0.2 mg/mL
缓冲液 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C,-20 °C
储存方法 Store the Insulin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

图像

Insulin抗体详情 Insulin目标详情 使用细节 贮存及处理 回页首
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Insulin (INS) (AA 1-84) antibody (ABIN3026326) IHC testing of human pancreas stained with Insulin antibody cocktail (E2-E3 + 2D11-H5).