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Phenotypic effects of antisense expression were identical for atp5 (显示 ATP5J ELISA试剂盒) and atp3. Reduced expression of either gene resulted in stunting of etiolated seedlings, downward curling or wavy-edged leaf margins and ball-shaped unexpanded flowers.
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and the central stalk which is part of the complex rotary element. The gamma subunit protrudes into the catalytic domain formed of alpha(3)beta(3). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.
ATP synthase gamma chain
, ATP synthase subunit gamma
, ATPsyn-gamma chain
, F0/F1 ATP synthase gamma
, F1ATP synthase
, catalytic core of F1 ATP synthase of complex V