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TNF alpha Protein (full length)

TNF alpha 宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant > 98 % pure Active
产品编号 ABIN935842
发货至: 中国
  • 抗原 See all TNF alpha 蛋白
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    full length
    宿主
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    资源
    • 58
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    大肠杆菌(E. Coli)
    产品特性
    Purified recombinant Human TNF alpha protein
    Expression System: E.coli
    Bioactivity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is
    纯化方法
    Proprietary chromatographic technique
    纯度
    > 98 % pure
    Top Product
    Discover our top product TNF alpha 蛋白
  • 应用备注
    Each Investigator should determine their own optimal working dilution for specific applications.
    实验流程

    Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 1.234 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
    2. Analysis by RP-HPLC, using a calibrated solution of TNF-a as a Reference Standard.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute in water to a concentration of 0.1 - 1.0 g/mL.
    缓冲液
    Lyophilized from 20 mM PBS, pH 7.2, with 10 mM NaCl.
    注意事项
    Avoid repeated freeze/thaw cycles.
    储存条件
    4 °C/-20 °C
    储存方法
    Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
  • Spatz, Eibl, Hink, Wolf, Fischer, Mayr, Schernthaner, Eibl: "Impaired primary immune response in type-1 diabetes. Functional impairment at the level of APCs and T-cells." in: Cellular immunology, Vol. 221, Issue 1, pp. 15-26, (2003) (PubMed).

  • 抗原
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    别名
    TNF alpha (TNF alpha 产品)
    别名
    DIF Protein, TNF-alpha Protein, TNFA Protein, TNFSF2 Protein, RATTNF Protein, Tnfa Protein, tnf Protein, TNF-a Protein, TNFalpha Protein, Tnfsf1a Protein, TNFa Protein, cTNF Protein, Tnf-alpha Protein, tnfa-like Protein, TNF-ALPHA Protein, dif Protein, tnfa Protein, xtnf Protein, tnfsf2 Protein, tnf-alpha Protein, Cachectin Protein, tumor necrosis factor Protein, tumor necrosis factor b (TNF superfamily, member 2) Protein, tumor necrosis factor alpha Protein, tumor necrosis factor a (TNF superfamily, member 2) Protein, TNF Protein, Tnf Protein, tnf Protein, tnfb Protein, tnf-alpha Protein, LOC103694380 Protein, tnfa Protein
    背景
    Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNF's primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
    Alternative Names: Tumor Necrosis Factor protein, TNFa protein, Cytotoxin protein, DIF protein, TNFSF2 protein, TNF-alpha protein, TNF-a protein, Differentiation-inducing factor protein, Necrosin protein, Cachectin protein
    分子量
    17,483 Da
    途径
    NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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