Claudin 6 Protein-VLP (CLDN6) (AA 1-220)
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- 抗原 See all Claudin 6 (CLDN6) 蛋白
- Claudin 6 (CLDN6)
- 蛋白类型
- VLP
- 生物活性
- Active
- 产品特性
- AA 1-220
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宿主
- 人
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资源
- HEK-293 Cells
- 应用范围
- ELISA, Immunogen (Imm), Functional Studies (Func), Surface Plasmon Resonance (SPR)
- 原理
- Human Claudin 6 Protein-VLP
- 序列
- Met1-Val220
- 产品特性
- Recombinant Full length Human Claudin 6 Protein-VLP is expressed from HEK293.It contains Met1-Val220.
- 纯度
- > 95 % as determined by HPLC.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Claudin 6 VLP at 5μg/ml (100μl/Well). Dose response curve for Anti-Claudin 6 Antibody, mFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.36 nM as determined in SPR assay (Biacore T200).
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Claudin 6 (CLDN6) (AA 1-220) (Active) protein-VLP
CLDN6 宿主: Cynomolgus 宿主: HEK-293 Cells VLP > 95 % as determined by HPLC ELISA, Imm, Func, SPR Active
Claudin 6 (CLDN6) (AA 1-219) (Active) protein-VLPCLDN6 宿主: 小鼠 宿主: HEK-293 Cells VLP > 95 % as determined by HPLC ELISA, Imm, Func, SPR Active
Claudin 6 (CLDN6) (AA 1-220) (Active) protein-VLP (Biotin)CLDN6 宿主: 人 宿主: HEK-293 Cells VLP > 95 % as determined by HPLC ELISA, Imm, Func, SPR Active
Claudin 6 (CLDN6) protein (mFc Tag)CLDN6 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Claudin 6 (CLDN6) (AA 1-220) protein (GST tag)CLDN6 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Claudin 6 (CLDN6) (AA 1-219) protein (Strep Tag)Crystallography grade CLDN6 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Claudin 6 (CLDN6) (AA 1-220) protein (Strep Tag)Crystallography grade CLDN6 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- 应用备注
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- Antibody Discovery: Immunization, Screening, Functional Characterization
- Affinity determination: ELISA, SPR
- In vivo pharmacokinetic analysis
- CMC method development
- CAR-T Positive Rate Detection
- Blood sample determination: ELISA
- 说明
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Virus-like particles (VLPs) are formed from the outer capsid protein of a virus and are tiny nanoparticles formed by the automatic assembly of one or more capsid proteins. VLPs do not contain viral infectious genomes, so they are relatively safe during production operations. The SAMS™ protein engineering platform has been used to express a series of biotinylated, non-biotinylated, and fluorescently-labeled VLP-displayed antigens. They are suitable for SPR, ELISA, CAR-T positive rate detection, and other experimental scenarios.
Virus-Like Particles (VLPs) are highly immunogenic, meaning that they can elicit a strong immune response in the host. VLPs are recognized by the immune system and are taken up by antigen-presenting cells (APCs) such as dendritic cells. Once taken up by APCs, VLPs are processed and presented to T cells, which can trigger the activation of B cells to produce antibodies against the displayed antigen. Because VLPs resemble the structure and composition of native viruses, they are highly effective at inducing both humoral and cellular immune responses.
Generally, VLPs range in size from approximately 20 to 200 nanometers (nm). Compared to a cell-based immunization approach, their smaller size can optimize the immune response to target the specific antigen displayed on the surface of the engineered VLPs. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Supplied as 0.22μm filtered solution in PBS ( pH 7.4).
Notice: If you need it for immunization, Do Not use any adjuvant. - 储存条件
- -80 °C
- 储存方法
- Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Claudin 6 (CLDN6)
- 别名
- Claudin 6 (CLDN6 产品)
- 别名
- cldn4l2 Protein, cldn6 Protein, CLDN6 Protein, AL024037 Protein, claudin 6, gene 1 L homeolog Protein, claudin 6 Protein, cldn6.1.L Protein, CLDN6 Protein, Cldn6 Protein
- 背景
- Claudin-6 is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. Human Claudin-6 shares 88 % and 86 % amino acid sequence identity with mouse and rat Claudin-6, respectively.
- 分子量
- 24.5 kDa.
- UniProt
- P56747
- 途径
- Hepatitis C
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