Adenosine A2a Receptor Protein-VLP (ADORA2A) (AA 1-412)
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- 抗原 See all Adenosine A2a Receptor (ADORA2A) 蛋白
- Adenosine A2a Receptor (ADORA2A)
- 蛋白类型
- VLP
- 生物活性
- Active
- 产品特性
- AA 1-412
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宿主
- 人
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资源
- HEK-293 Cells
- 应用范围
- ELISA, Immunogen (Imm), Functional Studies (Func), Surface Plasmon Resonance (SPR)
- 原理
- Human A2AR Protein-VLP
- 序列
- Met1-Ser412
- 产品特性
- Recombinant Human A2AR Protein-VLP is expressed from HEK293.It contains Met1-Ser412.
- 纯度
- > 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human A2AR VLP at 10μg/ml (100μl/Well) on the plate. Dose response curve for Anti-A2AR Antibody, hFc Tag with the EC50 of 0.87μg/ml determined by ELISA.
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Adenosine A2a Receptor (ADORA2A) (AA 1-412) protein (GST tag)
ADORA2A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Adenosine A2a Receptor (ADORA2A) (AA 147-267) protein (GST tag)ADORA2A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Adenosine A2a Receptor (ADORA2A) (AA 1-412) protein (Strep Tag)Crystallography grade ADORA2A 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Adenosine A2a Receptor (ADORA2A) (AA 286-410) protein (His tag)Crystallography grade ADORA2A 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Adenosine A2a Receptor (ADORA2A) (AA 291-412) protein (His tag)Crystallography grade ADORA2A 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Adenosine A2a Receptor (ADORA2A) (AA 1-410) protein (Strep Tag)Crystallography grade ADORA2A 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
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- 应用备注
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- Antibody Discovery: Immunization, Screening, Functional Characterization
- Affinity determination: ELISA, SPR
- In vivo pharmacokinetic analysis
- CMC method development
- CAR-T Positive Rate Detection
- Blood sample determination: ELISA
- 说明
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Virus-like particles (VLPs) are formed from the outer capsid protein of a virus and are tiny nanoparticles formed by the automatic assembly of one or more capsid proteins. VLPs do not contain viral infectious genomes, so they are relatively safe during production operations. The SAMS™ protein engineering platform has been used to express a series of biotinylated, non-biotinylated, and fluorescently-labeled VLP-displayed antigens. They are suitable for SPR, ELISA, CAR-T positive rate detection, and other experimental scenarios.
Virus-Like Particles (VLPs) are highly immunogenic, meaning that they can elicit a strong immune response in the host. VLPs are recognized by the immune system and are taken up by antigen-presenting cells (APCs) such as dendritic cells. Once taken up by APCs, VLPs are processed and presented to T cells, which can trigger the activation of B cells to produce antibodies against the displayed antigen. Because VLPs resemble the structure and composition of native viruses, they are highly effective at inducing both humoral and cellular immune responses.
Generally, VLPs range in size from approximately 20 to 200 nanometers (nm). Compared to a cell-based immunization approach, their smaller size can optimize the immune response to target the specific antigen displayed on the surface of the engineered VLPs. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Supplied as 0.22μm filtered solution in PBS ( pH 7.4).
Notice: If you need it for immunization, Do Not use any adjuvant. - 储存条件
- -80 °C
- 储存方法
- Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- Adenosine A2a Receptor (ADORA2A)
- 别名
- A2AR (ADORA2A 产品)
- 别名
- ADORA2A Protein, adora2a.1 Protein, zgc:194773 Protein, A2aR Protein, ADORA2 Protein, RDC8 Protein, A2AAR Protein, AA2AR Protein, A2ar Protein, ADENO Protein, Adora2l1 Protein, adora2a.2 Protein, zgc:193802 Protein, zgc:193822 Protein, adenosine A2a receptor Protein, adenosine A2a receptor a Protein, adenosine A2a receptor L homeolog Protein, adenosine A2a receptor b Protein, ADORA2A Protein, Adora2a Protein, adora2aa Protein, adora2a.L Protein, adora2a Protein, adora2ab Protein
- 背景
- Adenosine is a neuromodulator in the adult central nervous system. Membrane-bound receptors for adenosine have been identified and cDNAs for A1, A2a, A2b, and A3 adenosine receptor subtypes have been cloned recently.Expression of A2a adenosine receptor mRNA in cranial ganglia, carotid body, and intermediate lobe of the pituitary gland similarly suggests novel sites of adenosine action during development and in the adult.
- 分子量
- 45.5 kDa.
- UniProt
- P29274
- 途径
- Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints
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