CTLA4
(Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
蛋白类型
Recombinant
产品特性
AA 36-161
宿主
人
资源
HEK-293 Cells
标记
This CTLA4 protein is labelled with His tag.
原理
Human CTLA-4/CD152 Protein
序列
Lys36-Asp161
产品特性
Recombinant Human CTLA-4/CD152 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys36-Asp161.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CTLA-4, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 20.1ng/ml determined by ELISA. See testing image for detail.
CTLA4
宿主: 人
宿主: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
ELISA, FACS
CTLA4
宿主: 豚鼠
宿主: Yeast (Pichia pastoris)
Recombinant
> 90 % as determined by reducing SDS-PAGE.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
CTLA4
(Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
CTLA-4 Protein, CTLA4 Protein, CD152 Protein, Cd152 Protein, Ctla-4 Protein, Ly-56 Protein, CD Protein, CELIAC3 Protein, GRD4 Protein, GSE Protein, IDDM12 Protein, sCTLA4 Protein, cytotoxic T-lymphocyte associated protein 4 Protein, cytotoxic T-lymphocyte-associated protein 4 Protein, CTLA4 Protein, Ctla4 Protein
背景
CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation - a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.
分子量
14.3 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result.