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SARS-CoV-2 Spike Protein (B.1.1.7 - alpha) (His tag)

宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
产品编号 ABIN7013275
发货至: 中国
  • 抗原 See all SARS-CoV-2 Spike 蛋白
    SARS-CoV-2 Spike
    蛋白类型
    Recombinant
    生物活性
    Active
    产品特性
    B.1.1.7 - alpha
    宿主
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    SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha
    资源
    • 198
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    HEK-293 Cells
    标记
    This SARS-CoV-2 Spike protein is labelled with His tag.
    序列
    AA 16-1213
    特异性
    SARS-CoV-2 S protein (HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H), His Tag (MALS verified)
    产品特性
    SARS-CoV-2 S protein, His Tag is the ectodomain of SARS-CoV-2 S protein that contains AA Val 16 - Pro 1213 (Accession # QHD43416.1) and HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H mutations, which have become increasingly common in SARS-CoV-2 viruses from around the world. The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. HV69-70del / Y144del / N501Y / A570D / D614G / P681H / T716I / S982A / D1118H mutations were identified in the SARS-CoV-2 variant (known as 20B/501Y.V1, VOC 202012/01, or B.1.1.7 lineage) which emerged in the United Kindom.
    纯度
    >90 % as determined by SDS-PAGE.
    内毒素水平
    Less than 1.0 EU per μg by the LAL method.
    Top Product
    Discover our top product SARS-CoV-2 Spike 蛋白
  • 应用备注
    MALS verified
    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    PBS
    储存条件
    -20 °C
  • Longworth, Dittmar: "An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).

  • 抗原
    SARS-CoV-2 Spike
    别名
    SARS-CoV-2 S protein (SARS-CoV-2 Spike 产品)
    别名
    E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
    物质类
    Viral Protein
    背景
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
    分子量
    137.8 kDa
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