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SARS-CoV-2 Spike S1 Protein (RBD) (His tag)

Name: SARS-CoV-2 Spike S1 Protein (RBD) (His tag)
SKU: ABIN6952628
Availability: OutOfStock

宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE.
> 90 % as determined by SEC-MALS. SDS, ELISA

Immobilized SARS-CoV-2 S protein RBD, His Tag (ABIN6952628) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.

ELISA

SARS-CoV-2 S protein RBD, His Tag on SDS-PAGE under reducing (R) condition.

SDS

Immobilized Human ACE2, Fc Tag (ABIN6952465) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD, His Tag (ABIN6952628) with a linear range of 2-39 ng/mL (QC tested).

ELISA

3 images

(5 references)

产品编号 ABIN6952628

发货至: 中国

Datasheet as PDF

抗原 See all SARS-CoV-2 Spike S1 蛋白

SARS-CoV-2 Spike S1

蛋白类型

Recombinant

产品特性

RBD
宿主
95

9

5

4

2

2

2

1
SARS Coronavirus-2 (SARS-CoV-2)
资源
87

6

3

3

1

1

1

1

1
HEK-293 Cells

标记

This SARS-CoV-2 Spike S1 protein is labelled with His tag.

应用范围

SDS-PAGE (SDS), ELISA

原理

SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified)

序列

AA 319-537

产品特性

SARS-CoV-2 S protein RBD, His Tag is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1). Predicted N-terminus: Arg 319
This protein carries a polyhistidine tag at the C-terminus.

纯度

> 95 % as determined by SDS-PAGE.
> 90 % as determined by SEC-MALS.

过滤

0.22 μm filtered

内毒素水平

Less than 1.0 EU per μg by the LAL method.

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SARS-CoV-2 Spike S1 (RBD) protein (His tag,AVI tag,Biotin)
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SARS-CoV-2 Spike S1 (BA.4 - Omicron), (BA.5 - Omicron) protein (His tag)
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SARS-CoV-2 Spike S1 (BA.2.12.1 - Omicron) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

SARS-CoV-2 Spike S1 protein (Fc-Avi Tag)
宿主: SARS Coronavirus (SARS-CoV) 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

SARS-CoV-2 Spike S1 (N501Y) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

SARS-CoV-2 Spike S1 (E484K), (K417N), (N501Y) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
状态

Lyophilized

缓冲液

PBS, pH 7.4

注意事项

Please avoid repeated freeze-thaw cycles.

储存条件

4 °C,-20 °C,-80 °C

储存方法

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.
Sasisekharan, Pentakota, Jayaraman, Tharakaraman, Wogan, Narayanasami: "Orthogonal immunoassays for IgG antibodies to SARS-CoV-2 antigens reveal that immune response lasts beyond 4 mo post illness onset." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 118, Issue 5, (2021) (PubMed).

Narang, James, Balmer, Wilson: "Protein Footprinting, Conformational Dynamics, and Core Interface-Adjacent Neutralization "Hotspots" in the SARS-CoV-2 Spike Protein Receptor Binding Domain/Human ACE2 Interaction." in: Journal of the American Society for Mass Spectrometry, (2021) (PubMed).

Guo, Huang, Zhang, Yao, Zhou, Shen, Shen, Li, Li, Zhang, Chen, Chen, Wu, Fu, Zeng, Feng, Pi, Wang, Zhou, Lu, Li, Fang, Lu, Hu, Wang, Zhang, Gao, Adrian, Wang, Yu, Peng, Gabibov, Min, Wang, Huang et al.: "A SARS-CoV-2 neutralizing antibody with extensive Spike binding coverage and modified for optimal therapeutic outcomes. ..." in: Nature communications, Vol. 12, Issue 1, pp. 2623, (2021) (PubMed).

Longworth, Dittmar: "An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).

Wei, Wan, Yan, Wang, Zhang, Yang, Zhang, Fan, Li, Deng, Sun, Gong, Yang, Wang, Wang, Li, Yang, Li, Zhang, Wang, Du, Zong, Yin, Zhang, Wang, Peng, Lin, Feng, Qin, Chen, Gao, Zhang, Cao, Zhong: "HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry." in: Nature metabolism, Vol. 2, Issue 12, pp. 1391-1400, (2020) (PubMed).
抗原

SARS-CoV-2 Spike S1

Abstract

SARS-CoV-2 Spike S1 产品

别名

E2 Protein, Surface Glycoprotein Protein, S Protein

物质类

Viral Protein

背景

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

分子量

26.5 kDa

基因ID

43740568

UniProt

P0DTC2